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Yurnaliza, Yurnaliza
Universitas Sumatera Utara
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Journal : Bioscientist : Jurnal Ilmiah Biologi

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Skrining Actinomycetes Penghasil L-asparginase dari Pantai Olo, Sumatera Utara dan Uji Aktivitas Enzim Hartini, Martha Yeni Sry; Yurnaliza; Hastuti, Liana Dwi Sri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17133

Abstract

This research aims to isolate actinomycetes that produce the enzyme L-asparaginase from Olo Beach and to determine the activity of the L-asparaginase enzyme produced. Actinomycetes producing the enzyme L-asparaginase were obtained through the following steps: isolation on SCA medium using the spread plate method; selection of L-asparaginase-producing actinomycetes on M-9 liquid medium with phenol red as a color indicator; production of L-asparaginase based on the principle of fermentation in production medium, followed by measurement of L-asparaginase activity and protein content. Protein content was determined using the Lowry method with Bovine Serum Albumin (BSA) as the standard. The data obtained were analyzed using ammonia and BSA standard curves. The results showed that 11 actinomycetes isolates from Olo Beach were capable of producing L-asparaginase. Qualitative selection showed a change in media color from yellow to pink, indicating the presence of L-asparaginase activity. Quantitatively, the highest L-asparaginase enzyme activity was 40.688 U/mL and the highest specific activity was 123,677 U/mg, found in isolate SPO 6. The lowest enzyme activity was 31,651 U/mL and the lowest specific activity was 25,874 U/mg. Specific activity is influenced by enzyme activity and protein content.
Co-Authors Adrian Hartanto Aliyya Intan Misyilla Amelia Putri Aprilia Asri Bella Patricia Chairani Chairani Chairani Chairani Cheesa Priscilla Ronald Debi Setiawan Deddy Rusika Desy Christine Naibaho DWI SURYANTO Erman Munir Fachri Fauzi Fauzi, Fachri Hartini, Martha Yeni Sry Indriani Isnaini Nurwahyuni KIKI NURTJAHJA Langkah Sembiring LANGKAH SEMBIRING Liana Dwi Sri Hastuti Lubis, Nita A. Mahendra Kurniawan Manurung Mayang Sari Yeanny Nantika Indah Sari Nita A. Lubis Retno Widia Sari Ridha Ramana Riska Annisa Putri Rusika, Deddy Saleha Hannum Sebastian Margino Sebastian Margino Seny mahara Shanty Simanjuntak, Wira Risa Lina Simarmata, Wilander Sinaga, Riyanto Sri Dwi Hastuti Waty, Syahdiana Wita E Tarigan Yusman