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Warsidah, Warsidah
Program Studi Ilmu Kelautan, FMIPA Universitas Tanjungpura
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Journal : Journal of The Indonesian Society of Integrated Chemistry

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Fraksinasi dan Potensi Antioksidan dari Ekstrak Protein Kasar dari Spons Haliclona sp Asal Perairan Spermonde Sulawesi Selatan Warsidah, Warsidah; Sofiana, Mega Sari Juane; Safitri, Ikha; Nurdiansyah, Syarif Irwan
Journal of The Indonesian Society of Integrated Chemistry Vol. 15 No. 2 (2023): Journal of The Indonesian Society of Integrated Chemistry
Publisher : Pendidikan Kimia FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jisic.v15i2.25557

Abstract

Sponge Haliclona Sp is a member of the phylum porifera class Demospongia which is found living in the waters of the spermonde coral reefs of South Sulawesi. Its interaction with various microorganisms that live in the waters causes the formation of various kinds of active compounds and biological activities that are important in the world of health. The purpose of this study was to extract and fractionate protein from the Haliclona Sp sponge from Spermonde waters through confirmation using ammonium sulfate at various antioxidant levels, and to determine the content and activity of the protein in each fraction. The results showed that the protein content and antioxidant activity (IC50) of each fraction were, among others, in fraction 1 (0-30%) of 0.143 mg/L and 78.11 mg/L, fraction 2 (31-50%) 0.045 mg/L and 153.55 mg/L, fraction 3 (51–70%) of 0.020 and 161.12 mg/L, and fraction 4 (71–90%) of 0.112 and 104.71 mg/L, while crude protein of 0.277 mg/L and 115.22 mg/L. Ascorbic acid as a positive antioxidant control in this study resulted in an antioxidant activity of 2.74 mg/L.
Co-Authors . Apriansyah A. Kushadiwijayanto, Arie A.Nurrahman, Yusuf Abdul Hafiz, Iqbal Ade Hidayat, Ade Agustina, Sella Alfadzri, Andry Amelia, Debie Rizky Amran, Arman Andini, Dina Andryani, Semi Aninda, Dea Apriansyah Ari Widiyantoro Arief Nurrahman, Yusuf Aritonang, Anthoni B. Aritonang, Anthoni Batahan Ashari, Asri Mulya Astuti, Mega Sri Aswandi Aswandi, Aswandi Awlia, Isti Ayyash, Muhammad Yahya Bambang Kurniadi, Bambang BAMBANG SUJATMIKO Cahyany, Brigita Sari Kusuma Dahliana Dahliana dedi irawan Deni Deni Diah Wulandari Rousdy Dwi Gusmalawati Dzul Fadly Edwin Eny Enawaty Fadliah Nur, Andi Denisa Farhaby, Arthur M Fatmadiwi, Anggi Gusti Eva Tavita Haq, Izhar Amirul Hardan, Hardan Harianto Harianto, Miko Hasanah, Fadiah Amalia Helena, Shifa Hidayat, Maulana Idawati, Nora Ikha Ikha Safitri Irawan, Suhardi Irwan Irwan Nurdiansyah, Sy. Irwan Nurdiansyah, Syarif Izroil, Uwaiz Maulana Jayatri, Rahmi Jayuska, Afghani Juane, Mega Sari Junaidi, Levy Juniarti Juniarti Kalija, Tedi Ahmad Kasbutin, Hendri Kurniawanalamm Kushadiwijayanto , Arie Antasari Kushadiwijayanto, Arie Antasari Kusuma, Kwirinus Rio Kusumardana, Setra Laili Fitri Yeni Laksmitandari, Gania Ahlul Jannah Listy, Irma Lucky Hartanti, Lucky Lukas, Novan Luis Pernando Mardianto, Tomi Mardini, Delvia Devi Mariana Br. Surbakti Maryani Maryani Maser, Agnes Putri Masriani . Mega Sari Juane Sofiana Meidiantino, Stefans Melia Trianasta Minsas , Sukal Minsas, Sukal Muhardi Muhardi Muliadi Nabil, Ilhan Nail Neva Satyahadewi Nora Idiawati Noyanti, Resti Nur, Widia Nurdiansyah , Syarif Irwan Nurdiansyah, Irwan Syarif Nurdiansyah, Syarif Irwan Nurhamidah, Anggia Nurrahman , Yusuf Arief Nurul Asikin Oktavia Oktavia Oktavia Paulus Paulus Pratiwi, Mutiara Anugerah Prayitno, Dwi Imam Priani , Wina Puji Ardiningsih Putri, Kristina Meissy Andini Rafdinal Rafdinal Rahmat Rasmawan Ramadhan Sailendra, Pingkan Retno, Mayang Sari Rini Muharini Risko, Risko Rita Kurnia Apindiati Riza Linda Rody Putra Sartika Rudiyansyah Rudiyansyah S.J Sofiana, Mega Safitri , Ikha Sakina, Haiwatus Samira Samira Satyahadewi , Neva Selviana, Alya Siahaan, Jni Walbet Sihotang, Nesya Lia Simamora, Chinda Setia Lestari Sofiana, Mega Sj Sudiono, Gatot Sukal Minsas Sukardi Suparnawati, Suparnawati Supriwanti, Supriwanti Surya Darma Sy. Irwan Nurdiansyah Syamswisna , Tahirah Hasan Tengku Riza Zarzani N Tia Nuraya Triono, Riski WAHYUNI Waldi Widian Sari, Margareta Lita Yasnidar, Yasnidar Yuliono, Agus Yunivah, Adinda Yusuf Arief Nurrahman `B Aritonang, Anthoni