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Journal : Journal of Fisheries

Characteristics of Water-Soluble Collagen Extracted from Catfish (Pangasius sp.) Skin Using Different Acetic Acid (CH3COOH) Concentrations S.Pi., M. Si, Patmawati; Nuzil Romadhoni, Puput; Puspitaningsih , Devi; Sulmartiwi, Laksmi; Nirmala, Dwitha; Endi Rahmantyo, Lastiko; Moechthar, Oemar; Raseetha, Siva; Akmal Alwi Husein, Mohamad; Khadijah Zai
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.72086

Abstract

Graphical Abstract Highlight Research Optimized extraction enhances fish skin-based collagen as an alternative to bovine and porcine sources. Hydro-Extraction Advantage offer a cost-effective approach for producing high-purity, water-soluble collagen. Varying CH₃COOH concentrations influence collagen yield and solubility, impacting its potential applications in food, pharmaceutical, and cosmetic industries. Hydro-extracted collagen is biodegradable and free of harmful residues.     Abstract Collagen extraction from fish skin offers a sustainable approach to valorize fish processing by-products, and fish skin catfish (Pangasius sp.) is recognized as a promising collagen source. However, previous studies reported that in low water solubility, limiting its functional application in food, pharmaceutical, and cosmetic industries. To address this challenge, this study investigates the use of varying acetic acid concentrations (0.4, 0.6, and 0.8 M) during the hydrolysis stage prior to hydro-extraction, aiming to enhance the solubility and quality of the extracted collagen. Parameters measured included yield, solubility, proximate composition (protein, fat, air content, ash), organoleptic quality, molecular weight, amino acid profile and functional groups analysis. The best results were achieved with 0.6 M acetic acid, resulting in type I collagen coupled by the amide groups A, B, I, II, III, and molecular weights (65, 95, 130 and 270 kDa). The dominant amino acids identified was glycine. This treatment yielded a collagen extraction rate of 9.04% and solubility of 79.71%. The proximate composition included 67.34% protein, 14.87% fat, 8.48% moisture, and 10.69% ash. Organoleptic scores for appearance, odour, and texture were 7.80, 7.93, and 6.80, respectively. The collagen met the SNI 8076:2020 standard for protein content, moisture, and organoleptic attributes; however, fat and ash contents exceeded the specified limits. In conclusion, the acetic acid concentration significantly affects the physicochemical and sensory properties of collagen. Catfish skin shows strong potential as a raw material for collagen production, which supports its use in the food, pharmaceutical, and cosmetic sectors.
Effect of Ultrasonic Assisted Extraction with Ethanol for Removing Lipid on Catfish (Pangasius sp.) Skin as a Collagen Source and Its Characteristics Agustina, Maulida; Patmawati, Patmawati; Mubarok, Shofy; Sulmartiwi, Laksmi; Wulandari, Diah Anggraini; Zai, Khadijah; Siva, Raseetha; Pujiastuti, Dwi Yuli; Nirmala, Dwitha; Carattri Kusuma Werdani, Money; Moechthar, Oemar; Alwi Husein, Mohamad Akmal
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.46061

Abstract

Abstract Fish skin is rich in collagen (80%-90%). However, catfish (Pangasius sp.) skin has a high lipid content, which can reduce the quality of collagen. Therefore, treatment is needed to remove the lipid using ultrasonic assisted extraction (UAE) with ethanol. Hence, the aim of the present study was to remove lipid content from catfish skin as a raw material for collagen by using UAE with different concentrations of ethanol (25, 50, and 75%). The research was conducted in three stages: removing impurities, collagen extraction, and characterization of collagen. The result exhibited that UAE-ethanol treatment was capable of removing up to 85.6% of lipid content, and the produced collagen had the potential to be utilized as an alternative source of collagen based on its properties. Highlight Research Ethanol could be used as extractor of lipid from catfish skin of Pangasius Ultrasonic assisted extraction could increase efficiency of lipid extraction from catfish skin. Fish skin of Pangasius sp. has a potential source for collagen. Collagen from skin of Pangasius sp. has a good characteristic with high protein content.