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All Journal JMM (Jurnal Masyarakat Mandiri) JURNAL FARMASIMED (JFM) EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Krisdianilo, Visensius
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Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Jurnal Pengmas Kestra (JPK)

 1 
SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT: SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT Rizky, Vincentia Ade; Siregar, Saadah; Rahayu, Asvia; Krisdianilo, Visensius
JURNAL PENGMAS KESTRA (JPK) Vol. 2 No. 2 (2022): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.259 KB) | DOI: 10.35451/jpk.v2i2.1432

Abstract

Broiler chicken meat is a food source of animal origin that is widely consumed in Indonesia. The affordable price makes the demand for broiler chicken meat increasing. Chicken meat is easily contaminated by various microorganisms from the surrounding environment. Several types of microbes that can contaminate meat include E. Coli bacteria, Clostridium botulinum bacteria, Clostridium Perfringens, Salmonella bacteria, Staphylococcus aureus, and Anthrax. Microbial contamination in the form of Salmonella bacteria is generally found in broiler chicken meat. To overcome microbial contamination, the researchers conducted research on the effect of ginger on reducing the number of bacteria in chicken meat. This socialization aims to provide information to the public to be able to use ginger as an inhibitor and reduce the number of pathogenic bacteria and food destroyers in chicken meat. The results of this socialization were obtained from previous research which concluded that a decrease in the number of bacteria was due to the administration of ginger. This is because ginger contains secondary compounds such as phenols, treponoids and benzaldehyde which are bacteriostatic so that they function to inhibit the growth of microorganisms. The results of the socialization carried out in the community around Lubuk Pakam stated that they understood the processing and storage techniques (100%).
Workshop Diagnosis of Escherichia coli on Well Water Samples Krisdianilo, Visensius; Vincentia Ade Rizky; Saadah Siregar; Rahayu, Asvia
JURNAL PENGMAS KESTRA (JPK) Vol. 3 No. 1 (2023): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v3i1.1725

Abstract

Diarrhea is an endemic disease in Indonesia and is also a disease that can potentially cause extraordinary events that are often accompanied by death. Escherichia coli is a commensal bacterium, intestinal pathogen and extraintestinal pathogen that can cause urinary tract infections, meningitis, and septicemia. Most of the E. coli bacteria are in the digestive tract of animals as well as humans and are normal flora, but some are pathogenic that can cause diarrhea in humans (Bettelheim, 2000). Water is very potential in the spread of various diseases, for example water contaminated by pathogens and drunk by humans can cause disease (water born disease). Food and water contamination has been identified as a potential source of the spread of Escherichia coli pathogenicity in humans. The method has the disadvantage of taking a long time, a large number of samples, and an incorrect reading method of results. PCR technique is one of the molecular techniques used to identify infectious diseases caused by Escherchia coli. This method has many advantages that can produce accurate, fast, specific product amplification, requiring a small number of samples. and this method can be used to overcome the weaknesses of conventional diagnostics (cultures). The purpose of this study was to socialize the detection of Escherichia coli bacteria in well water samples by culture and PCR methods. From the examination that has been done obtained the results of cultural examination obtained colonies that grow on the medium Mac Conkey To appear round, flat edges, smooth surface with the color of the colony, then the results of pcr examination obtained 239 bp, which means bacteria found from the isolation of well water samples in the bottom of the identic pakam with Escherichia coli.As well as workshop participants can follow and understand the materials submitted and also the examination procedures carried out.
Socialization of Characterization and Molecular Identification of Protease Enzyme-Producing Bacteria from Tempeh Traded in Lubuk Pakam Market Krisdianilo, Visensius; Bukit, Sari Theresia
JURNAL PENGMAS KESTRA (JPK) Vol. 3 No. 2 (2023): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v3i2.1991

Abstract

Enzymes are an important ingredient in industry and health.  There are several types of enzymes that are widely used by humans such as cellulase enzymes, lipase enzymes, amylase enzymes and protease enzymes. Protease enzymes are enzymes that can accelerate the breaking of protein peptide chains. This enzyme is one of the most widely used enzymes in the industry with sales value reaching 60% of world enzyme sales. Very many industrial products use enzymes with enzyme demand that continues to increase. Total sales of enzymes in the industry in 2010 reached 3.3 billion US dollars and will continue to increase in the coming years.Protease enzymes can be isolated from plants, animals and microorganisms. The use of microorganisms in producing protease enzymes is very potential due to the very fast growth of microorganisms and can be grown on fairly cheap media. The purpose of this socialization is to provide information related to the examination of bacterial molecules producing protease enzymes in tempeh. From the activities carried out, it can be concluded that the presence of bacteria in tempeh sample S-2 which has the largest diameter is identical to the bacterium Pseudomonas stutzeri, while from the results of the participants' understanding, the results of the pre-test dominant understanding are sufficient (60%) and the results of the post-test results are dominantly good understanding (67%) which means that seminar participants are able to understand the material presented.
Workshop on The Use of Miana Leaf Juice (Coleus Scutellarioides (L) Benth) as An Alternative to Safranin Dye in Bacterial Gram Staining Krisdianilo, Visensius; Siregar, Sa'adah; Rahayu, Asvia; Rizky, Vincentia Ade
JURNAL PENGMAS KESTRA (JPK) Vol. 4 No. 1 (2024): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v4i1.2198

Abstract

One of the commonly used stains in bacterial identification is gram staining, this staining is used to distinguish the physical properties of the cell wall and the chemical properties of bacteria, this dye will distinguish bacteria into gram-positive bacteria with the result of the bacteria will be purple and gram negative with the result of red bacteria. The use of safranin is effective in working on bacteria because it is alkaline, this can cause binding between the chromophore component in the dye and the cytoplasm of bacteria that are basophilic. However, the use of safranin also has a negative impact on health and the environment. Therefore, the need to use materials that are safer for health and the environment, miana leaf juice (Coleus Scutellarioides (L) Benth) can be used as a natural dye to replace safranin. Minana leaves are very easy to find in gardens and are usually ornamental plants, the anthocyanin content in this plant can be used as a color pigment to replace safranin, anthocyanins will produce red to dark red pigments in an acidic atmosphere. The purpose of this community service activity, the community service organized a workshop for students of the Medical Laboratory Technology Study Program, Lubuk Pakam Medical Health Institute, aiming to provide information on the use of miana leaf juice (Coleus scutellarioides (L) Benth) as an alternative to safranin dye in bacterial gram staining. From the results of the service, it can be concluded that the participants can understand the material and the demonstration, this can be seen from the increase in the percentage of understanding both from before 61% to 87% after being given the demonstration and material.
Co-Authors Anisa Khairiyah Anna Teresia Marbun, Romauli Bambang Sumantri Bukit, Sari Theresia Ch, Lidwina Septie Febriady, Andy Haiti, Margareta HASTARI NINGRUM, DIAN Herlina Herlina Hutabarat, Mustika Sari H Hutabarat, Sintya Jayanti, Neti Eka Kartini . Lubis, Agnes Felicia Nopita Sari Manalu, Dian Rahayu, Asvia Riska Wahyuni Saadah Siregar Saadah Siregar, Saadah Sidabutar, Rimayani Siregar, S'aadah Siregar, Sa'adah Siregar, Sa’adah Siregar, Sa’adah Sitanggang, Morischa Deyla Syafrina Ginting, Suventi Vincentia Ade Rizky Vincentia Ade Rizky Yusnita, Desi