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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Sain Veteriner Buletin Peternakan Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Kedokteran Hewan Annales Bogorienses
Kaiin, Ekayanti Mulyawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : Annales Bogorienses

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Development of Mice Embryo (Mus musculus L.) after Closed Pulled Straw Vitrification in CZB Medium Gunawan, Muhammad; Kaiin, Ekayanti Mulyawati; Rusherdiannita, Raden Cindy; Alipin, Kartiawati
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
Publisher : BRIN

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The objective of our present experiment was to investigate the effects of closed pulled straw (CPS) vitrification on the viability and development of mouse embryo. The experiment was arranged according to completely randomized design (CRD) consisting of 4 treatments, namely not vitrification (NV) is control, CPS 1 (mCZB Hepes + 20% Bovine Serum Albumin (BSA) +0.5 M sucrose + 10% EG + 10% DMSO), CPS 2 (mCZB Hepes + 20% BSA + 0.5 M sucrose + 15% EG + 15% DMSO), and CPS 3 (mCZB Hepes + 20% BSA +0.5 M sucrose + 20% EG + 20% DMSO) with 6 replications. The viability of embryos (%) was determined after 24 - 72 h of the culture period, while we also observed the percentage of embryos reaching the blastocyst stage (early blastocyst, expanded, hatching, and hatched). As a result, the treatments did not give a significant difference in the viability of embryos (P<0,05) but showed significant effects on embryo development (P<0,05). Furthermore, this present work conclusively found that CPS vitrification in CZB medium with cryoprotectants ethylene glycol (EG) and dimethyl sulfoxide (DMSO) noticeably influenced the development of mice embryo to reach the blastocyst stage, but showed no remarkable difference in the viability of embryo after culture for 24 – 72 h.
Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

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The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Co-Authors ACHMAD FARAJALLAH Ananda, Ananda Bambang Purwantara Dyah Perwitasari Faisal Amri Satrio Ferdy Saputra Hariono Hariono Hasbi Hermansyah, Alfandy Herry Sonjaya ITA DJUWITA Kartiawati Alipin Kurnia, Asep Kusdiantoro Mohamad Kuswati Kuswati M Agus Setiadi Madihah Madihah, Madihah Maulana, Tulus Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Muhammad Gunawan Mutmainna, Mutmainna Nafisah, Ghina Ni Wayan Kurniani Karja Niken Ulupi Nurkarimah, Dona Astari Paskah Partogi Agung Qhatimah, Husnul Rudi Afnan Rusherdiannita, Raden Cindy Saputra, Wandi Sri Gustina Sudirman Baco, Sudirman Syahruddin Said Tri Eko Susilorini Tuti Suryati TUTY LASWARDI YUSUF Wahono Esthi Prasetyaningtyas