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All Journal Jurnal Veteriner Media Kedokteran Hewan Aceh Journal of Animal Science Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology LOSARI: Jurnal Pengabdian Kepada Masyarakat Bima Abdi: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner
Rahmahani, Jola
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60115
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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Journal : Jurnal Kedokteran Hewan

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Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Asri Rizky; Mustofa Helmi Effendi; Jola Rahmahani
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.571 KB) | DOI: 10.21157/j.ked.hewan.v15i2.4817

Abstract

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.
Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Rizky, Asri; Effendi, Mustofa Helmi; Rahmahani, Jola
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.4817

Abstract

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cows milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cows milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.
Co-Authors A'la, Rofiqul A. T. Soelih Estoepangestie Adikara, Tatang Santanu Agustono, Bodhi Akbar, Daniswara Irwanza Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Ali Agus Amal, Mohammad Noor Azmai Amelia, Ezanti Nur Aprinda Ratna Lovela Ardhanella, Sellianova Arif Nur Muhammad Ansori Asri Rizky Asri Rizky Astria Novitasari Nidom Astuti, Elita Sriwidya Aswin, Ahmad Azahro, Aisyah Aziz, Githa Nurma Balqis Afifah Bambang Sektiari Lukiswanto Baroroh, Diana Nova Boedi Setiawan BUDI UTOMO Christian Marco Hadi Nugroho Chusniati, Sri Dhani Prakoso Didik Handijatno Didik Handijatno Diyantoro Diyantoro, Diyantoro Elisa Herina Dimariwu Erma Safitri Ernawati, Rahaju Ernawati, Rahayu Fachriza Tri Aprilia Fachrur Rozi Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Fidyah Fitrawati, Fidyah Haeriah Hani Plumeriastuti Harijani, Nenny Helen Susilowati, Helen Herry Agoes Hermadi Hutahaean, Ryan David Pandapotan IMAM MUSTOFA Imam Mustofa Indah Rahmawati Ine Karni Ira Sari Yudaniayanti Irfani, Dyah Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Karo-karo, Desniwaty Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Maha Kuncoro Puguh Santoso Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasari, Pitaloka Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lucia Tri Suwanti, Lucia Tri Meles, Dewa Ketut Mohammad Sofi’ul Anam Muhammad Arief Muhammad Khaliim Jati Kusala Muhammad, Abdul Latif Mustofa Helmi Effendi Nidom, Chairul Anwar Nor Laela Ramadhaniyah Nurwartanti, Maya Nusdianto Triakoso Pasang, Gabriel Sampe Pinayungan, Probo Probo Warih Tatag Pradhita, Dewanggi Kristi Sasmi Praja, Ratih Novita Prestalia Dwi Rachmawati Putih, Nurvita Putri, Diana Novita Rahaju Ernawati Rahaju Ernawati Rahmawati, Anlisia Rahmawati, Kadek Rahmi Sugihartuti Ramandinianto, Sancaka Chasyer Ratna Damayanti Reviany Vibrianita Nidom Rimayanti Rimayanti Rizky, Asri Rosyta, Pegy Rozi Sabdoningrum, Emy Koestanti Salleh, Annas Sari, Putri Desi Wulan Setyarina Indrasari Siti Komariyah Soeharsono Soeharsono Soeharsono Supriyadi Supriyadi Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Tacharina, Martia Rani Tiara Wandira Hariyanto Tita Damayanti Lestari Tyasningsih, Wiwiek Tyasningsih, Wiwiek Venri Novryantoro Vensko Zico Dandi Wahyu Pratama Wibawati, Prima Ayu Widjaja, Nanik Sianita Wijaya, Andi Yasmin Wijaya, Nanik Sianita Wiwiek Tyasningsih Wiwik Misaco Yuniarti Wudhu, R Zaksara Fero Ali Mutaqin Wulandari, Innah Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zayyin Dinana Zulfanisa, Rizky Asrin Zulianti, Brillia