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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia International Journal of Renewable Energy Development Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Pangan dan Agroindustri Biota Planta Tropika EDUFORTECH Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Teknologi & Industri Hasil Pertanian Menara Perkebunan International Journal of Engineering, Science and Information Technology Agroradix : Jurnal Ilmu Pertanian International Journal of Renewable Energy Development
Mulyorini Rahayuningsih
Department Of Agroindustrial Technology, Faculty Of Agricultural Techonology, Bogor Agricultural University, Darmaga Campus, Bogor, 16680
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Transportation

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
Penggandaan Skala Produksi Bioinsektisida Bacillus thuringiensis var. israelensis untuk Membasmi Jentik Nyamuk Aedes aegypti Mulyorini Rahayuningsih; Khaswar Syamsu; Abdul Aziz Darwis; Rini Purnawati
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.727 KB)

Abstract

The objective of this research is to study the scaling up of bionsecticide production from Bacillus thuringiensisvar. israelensisusing onggok (a cassava by-product) as a carbon source. The insecticide produced was used to eradicate Aedes aegypti larvae. The product was a crystal protein produced during bacterial sporulation. Scaling up from laboratory to pilot plant scale was done using two methods, i.e. constant agitation power per unit volume (Pg/V) and constant oxygen transfer coefficient (kLa). The results showed that yield of product per substrate (Yp/s) of Pg/V based product with the value of 3.52 ± 0.02 spora per gram substrates was higher than Yp/s of  kLa based product with the value of 2.96 spora per gram substrate.  Logarithmic value of viable spore count (log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as compared to 7.17 ± 0.20 for kLa based product. Substrate efficiency was also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of Pg/V based product was lower (0.49 μg/ml) meaning that it was more toxic than kLa based product (0.62 μg/ml). Amino acid content of Pg/V based product was also higher than kLa based product. Constant Pg/V method was suggested as a based on the scaling up of bioinsecticide production of B. thuringiensis israe/ensison industrial scale. Keywords: bioinsecticide, Bacillus thuringiensisvar. israelensis, kLa, Pg/V, LC50, viable spore count
Penapisan dan Karakterisasi Amilase dari Bakteri Asal Ekoenzim Ninda Ningtyas; Nisa Rachmania Mubarik; Mulyorini Rahayuningsih
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 3 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.3.441

Abstract

Eco-enzymes are one of the potential sources for obtaining amylolytic bacterial isolates. The study aims to screen amylolytic bacteria from eco-enzymes, characterize semi-purification amylase, and identify amylolytic bacteria molecularly using the 16S rRNA gene. Screening for amylolytic bacteria was carried out by measuring the amylolytic index on a Nutrient agar medium containing 1% tapioca starch. The amylolytic isolate which had the highest index and was non-pathogenic was selected for the amylase characterization process. Testing of amylase activity was carried out using the Bernfeld method while the protein enzymes were measured using the Bradford method. The extracellular extract was concentrated using ammonium sulfate precipitation. PKL2 is gram-positive bacteria that was derived from eco-enzymes with the highest amylolytic indexes of 1.77, which were not pathogenic on the blood agar test. Optimum amylase was produced by PKL2 at the stationary phase at 21 h. The optimum pH and temperature of the amylase activity were observed to be 7.0 and 50°C, respectively. The amylase enzyme from PKL2 increased its purity 1.82-fold upon precipitation of ammonium sulfate at a concentration of 60%. Identification of bacteria based on molecular identification showed that PKL2 obtained was putatively identified as Bacillus amyloliquefaciens. Keywords: Amylase activity, Bacillus amyloliquefaciens, eco-enzyme, optimum pH
Bioprospecting of Pectinase-Producing Bacteria from Marine Actinomycetes Hasanah, Neneng; Nadhifah, Hana; Rahayuningsih, Mulyorini; Atikana, Akhirta; Ratnakomala, Shanti; Lisdiyanti, Puspita; Rahmani, Nanik
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.689

Abstract

Pectinase is used in a variety of industries, including fruit juice production, textile processing, papermaking, biodegumming, coffee and tea manufacturing, medicines, feed, and nutraceuticals. The demand for pectinase enzymes grows year after year. Industrial applications require high-activity enzymes that can increase considerable product yields while also improving based on needs and byproduct use. Indonesia's industry continues to rely on imported pectinase enzymes. Indonesia, on the other hand, possesses megabiodiversity, particularly marine microorganisms, which have the potential to provide new enzymes with high activity for industrial applications. This work aims to undertake bioprospecting of marine actinomycetes producing pectinase enzymes that have the capacity to hydrolyze pectin polymer, both from commercial and biomass sources. A total of 20 marine actinomycetes isolates from sediment, seawater, and sponges were bioprospected, and one isolate was selected with high pectinase activity (BLH 1.20), which was then used to characterize pH, temperature, hydrolysis analysis on pectin polymers, and isolate identification using 16s rRNA analysis. The selected isolate (BLH 1.20) performed best in a sodium phosphate buffer with a pH of 6.0 and a temperature of 30°C, achieving an activity of 5.4 U/mL. The 16S rRNA analysis revealed that the isolate is from the genus Streptomyces and the species Streptomyces sampsonii. Keywords: bioprospecting, marine Actinomycetes, pectinase, Streptomyces sampsonii
Co-Authors Abdul Aziz Darwis Alan, Jesslyn Alvina Amalia, Kiki Puspita Ati Atul Quddus Atikana, Akhirta Devi Ambarwaty Oktavia Djumali Mangunwidjaja Dwi Setyaningsih Elisa Anggraeni, Elisa Erliza Hambali Erliza Noor Fadhlurrakhman, Ariq Rizky Faidzin, Muhammad Nur Febilian Adiwinata Febilian Adiwinata Fidriyanto, Rusli Fitri, Ainissya Iftachul Farida Ika Agustin Rusdiana Ika Agustin Rusdiana Ika Amalia Kartika Jaini Fakhrudin Kartika Kartika Khaswar Syamsu Kirana Sanggrami Sasmitaloka Michael Silaen Mohamad Yani Nadhifah, Hana NANIK RAHMANI Neneng Hasanah Ninda Ningtyas NISA RACHMANIA MUBARIK Nugraha, Bambang Arif PUSPITA LISDIYANTI Rini Purnawati RONI RIDWAN Rosa, Mutya Rozalia Rozalia Rusdiana, Ika Agustin SHANTI RATNAKOMALA Singgih Wibowo Slamet Budijanto Suprihatin Suprihatin Suprihatin Suprihatin Talapessy, Cornelia Titi Candra Sunarti