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Journal : Jurnal Penelitian Hasil Hutan

ANALISIS TOKSISITAS BEBERAPA TUMBUHAN HUTAN DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) Zuraida
Jurnal Penelitian Hasil Hutan Vol. 36 No. 3 (2018): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2018.36.3.239-246

Abstract

Utilization of forest plant seeds of suren (Toona sureni (Blume) Merr.), mimba (Azadirachta indica A.Juss.), mahogany (Swietenia mahagoni (L.) Jacq.), and saga (Adenanthera pavonina L.) for curing certain illness have long been practiced by traditional community. However, there were limited study on observing seeds bioactivity of those four species. This study aimed to analyze the toxicity activity of suren, mahoni, mimba, and saga seeds. The four seeds were initially macerated using 96% ethanol. The ethanol extracts were then tested for their toxicity effects on the Artemia salina larvae by Brine Shrimp Lethality Test (BSLT) method. BSLT results were presented through the Lethal Concentration 50 (LC50). The LC50values of ethanol extract of suren seeds, mahogany, mimba, and saga were 75, 84, 323 and 449 μg/mL, respectively. The four extracts had biological activity with LC50<1000 μg/mL, and suren seed extract was the most likely to have bioactivity because it had the lowest LC50value.
FENOL, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN PADA EKSTRAK KULIT BATANG PULAI (Alstonia scholaris R.Br) Zuraida; Sulistiyani; Dondin Sajuthi; Irma Herawati Suparto
Jurnal Penelitian Hasil Hutan Vol. 35 No. 3 (2017): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2017.35.3.211-219

Abstract

Alstonia scholaris R.Br belongs to family Apocynaceae, is one of medicinal forest plant as traditional medicine to treat fever, malaria, cough with phlegm, diarrhea, diabetes, cholesterol-lowering, intestinal worms, acute rheumatism, ulcers, and hypertension. One of the causes of heart disease, atherosclerosis, and cancer is oxidative stress. The stress can be cured or reduced by taking antioxidant. Flavonoid, a phenol compound class, is one of the secondary plant metabolites that function as antioxidant. This paper determines total levels of phenol, flavonoid, and antioxidant activity of Alstonia *stem bark extract (Alstonia scholaris R. Br). Quantitative determination of total phenolics with the folin-ciocalteu method expressed as mg of gallic acid equivalent (GAE) per gram of extract, total flavonoids content by AlCl₃ method expressed as Quercetin equivalent (QE), and in vitro antioxidant activities with DPPH (2,2-diphenyl-1-picrylhydrazyl) method expressed in terms of IC₅₀ (inhibition concentration). Results show that extraction of three replicates in maceration with 96% ethanol yielded 4.19% filtrate. The total phenolic content was 51.50 mg GAE /g extract, while the total flavonoid content was 0.35 mg QE /g extract. IC₅₀ value of antioxidant activity assay of stem bark extract was 211.54 µg/mL.