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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abdidas Jurnal Riset Pendidikan MIPA Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi
Ashari, Abdul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Bioscientist : Jurnal Ilmiah Biologi

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Hubungan Pola Makan dan Aktivitas Fisik dengan Status Gizi Siswa Kelas VIII SMP Negeri 19 Palu Asifah, Nur; Laenggeng, Abd. Hakim; Lilies, Lilies; Kundera, I Nengah; Masrianih, Masrianih; Ashari, Abdul
Bioscientist : Jurnal Ilmiah Biologi Vol 13, No 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.16623

Abstract

This study aims to analyze the relationship between diet and physical activity with the nutritional status of students of SMP Negeri 19 Palu. This study used a cross sectional design with a quantitative approach and analytic observational type. The subjects were 77 students of class VIII (aged 13-15 years) who were selected using simple random sampling technique. Data were collected using FFQ (Food frequency Questionnaire) to assess diet and APARQ (Adolescent physical Activity Recall Questionnaire) for physical activity, both validated instruments from previous studies. As well as anthropometric measurements (height and weight) using the Body Mass Index (BMI) to determine nutritional status. The results of the analysis using the chi-square test showed that there was a significant relationship between diet and nutritional status (χ²(3) = 8.772; p = 0.032), and between physical activity and nutritional status (χ²(4) = 9.919; p = 0.042) Respondents with a good diet and moderate to vigorous physical activity tended to have normal nutritional status, while poor diet and light physical activity were more common among students with abnormal nutritional status. These findings suggest that diet and physical activity play an important role in determining the nutritional status of adolescents. The implications of this study emphasize the importance of educational intervention programs in the school environment to support healthy lifestyles and prevent nutritional problems among students.
Identifikasi Jamur dan Bakteri Pada Plup Proses Fermentasi Biji Kakao Tandi, Jenifa Arisa; Kundera, I Nengah; Windarsih, Yulia; Alibasyah, Lestari M.P; Ashari, Abdul; Budiarsa, I Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17537

Abstract

This study aims to identify the types of fungi and bacteria that play a role in the fermentation process of cocoa beans. This type of research uses an exploratory laboratory method. The samples in this study were fungi and bacteria during the fermentation process of cocoa beans that had been fermented for approximately 3-5 days. The tools used in this study were digital scales, clear plastic boxes, autoclaves, scissors, micropipettes, spoons, label paper, Petri dishes, incubators, glass covers, Bunsen burners, Erlenmeyer flasks, inoculation needles, stirring rods, microscopes, beakers, measuring cups, scales, hot plates, test tubes, spirit lamp, colony counter, aluminum foil, camera, and writing instruments. The materials used in this study were fermented cocoa bean waste samples colonized by fungi and bacteria, Nutrient Agar (NA) medium, Triple Sugar Iron Agar (TSIA) medium, Bacillus Agar Base, distilled water, crystal violet, eosin dye, iodine, alcohol, and safranin. There are five stages in this research procedure, namely sample collection and preparation, media preparation and sample dilution, sample cultivation on general and selective media, Gram staining, and biochemical testing. The results of this study show that the fungi Saccharomyces cerevisiae and Mucor Sp, as well as the bacteria Bacilllus Sp, are involved in the fermentation process of cacao beans.
Analisis Filogenetik Cryptic species Apis cerana Fabricius, 1793 Berdasarkan Gen 16S rRNA Puspitasari, Amira; Budiarsa, I Made; Ashari, Abdul; Dhafir, Fatmah; Windarsih, Yulia; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14967

Abstract

Apis cerana is a member of the Insecta class grouped in the order Hymenoptera. Phylogenetic analysis is used to describe the kinship of living things through phylogeny trees. This study aims to describe the phylogenetic relationship of cryptic species in Apis cerana based on the 16S rRNA gene. Sampling use the cruising method in various regions, the DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The result showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationship. A. cerana from Central Sulawesi is more closely related to other A. cerana species in the same clade, while other species are separated. The result of this study show a clear pattern of kinship between A. cerana populations.
Analisis Filogenetik Lebah Madu Endemik Sulawesi Apis nigrocincta Smith, 1860 Berdasarkan Gen 16S rRNA Hidyanisa, Dhea; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Nurdin, Musdalifah; Windarsih, Yulia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14968

Abstract

Apis nigrocincta is an endemic honey bee that is only found in two regions in Indonesia, namely the Sangihe Islands and Sulawesi Islands. Phylogenetic analysis is used to describe the phylogenetic relationship of Sulawesi endemic honey bee A. nigrocincta based on the 16S rRNA gene. Sampling using the cruising method in various regions, then DNA isolation, DNA amplification, sequencing, and continued bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationships. A. nigrocincta from Central Sulawesi is more closely related to other A. nigrocincta species in the same clade, while other species are separated. The results of this study show a clear pattern of kinship between A. nigrocincta populations. 
Analisis Filogenetik Lebah Tukang Kayu Xylocopa appendiculata Smith, 1852 Berdasarkan Gen Cytochrome Oxidase 1 (CO1) Rahayu, Irma Septi; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Masrianih, Masrianih; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14969

Abstract

Xylocopa appendiculata  is an insect from the order Hymenotera and has bright yellow morphological characteristics on the thorax, while the rest of the body is black. Phylogenetic analysis is used to describe the kinship of living things throughh phylogeny trees. This study aims to describe the phylogenetic relationship of carpenter bees Xylocopa appendiculata based on the cytochrome oxidase 1 (CO1). DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis using Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates a clear evalutionary relationship X. appendiculata from Central Sulawesi is more closely related to other X. appendiculata species in the same clade. The results of this study show a clear kinship pattern between populations of X. Appendiculata.
Identifikasi Pakan Lebah Madu Endemik Sulawesi Apis nigrocincta (Smith, 1861) Hamsa, Hamsa; Masrianih, Masrianih; Trianto, Manap; Ashari, Abdul; Windarsih, Yulia; Nurdin, Musdalifah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15190

Abstract

The study aims to identify the main feed source for Apis nigrocincta through the analysis of polent morphology. The study uses explorative descriptive methods with qualitative and quantitative approaches. The focus of this study is the identification of feed source for endemic honeybees of Sulawesi, Apis nigrocincta through the analysis of polent morphology. Through the approach of polent morphology analysis, successfully identified 12 plant species from 9 families that could potentially be feed sources such as, Cucurbita maxima, Clematis viticella, Clematis montana, Pseuderanthemum tropurpureum, Cersicaria odorata, Solamun torvum, Solamun nigrum, Pine mercussii, Piper betle, Piper nigrum, and Galium aparine identification showed the presence of polyn diversity that supports the nutritional needs of bees, and emphasized the importance of local vegetation conservation to maintain the sustainability of the A. nigrocincta population. The results of the study showed that the polen has characteristics such as the polish unit, size, shape, shape on the P and E side, aperture type, as well as exin ornamentation. The polents collected by bees A. nigrocincta come from various types of plants, namely fruits, ornamental plants, vegetable plants, herb plants, and trees. This diversity reflects the flexibility of bees in choosing food sources based on availability in the surrounding environment. The results of the identification of the polymer samples showed that 12 species of plants that are the main feed source for A. nigrocincta bees are sourced from the polen. Each species has different flower characteristics, both in terms of shape and color. Polen diversity indicates that the environment around Jononunu Village has a rich ecosystem with different types of flowering plants.
Hubungan Kekerabatan Kupu- Kupu (Lepidoptera) Berdasarkan Karakter Morfologi di Desa Pombewe, Sulawesi Tengah Artha, Fandy; Dhafir, Fatmah; Ashari, Abdul; Trianto, Manap; Bustamin, Bustamin; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15309

Abstract

This study aims to analyze the kinship of butterflies (Lepidoptera) based on morphological characters in Pombewe Village, Central Sulawesi. The research method used was the roaming method with free collection technique. Morphological characters observed included antenna shape, wing color and pattern, and body size. Kinship analysis was conducted using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) with the help of Paleontological Statistics software (PAST 4.03). The results identified 13 butterfly species from the Nymphalidae family, namely Euploea redtenbacheri, Euploea algea, Euploea hewitsonii, Danaus genutia leucoglene, Cyrestis strigata, Danaus genutia genutia, Euploea eunice, Vindula erota, Doleschallia polibete, Acraea terpsicore, Ideopsis juventa, Parantica cleona, and Cyrestis paulinus. The relationship dendrogram showed two main clusters with four relationship categories, namely very close relationship (Cyrestis strigata and Cyrestis paulinus, IS = 100%), close relationship (Euploea hewitsonii and Danaus genutia leucoglene, IS = 74.07%), not close relationship (Cyrestis strigata and Euploea eunice, IS = 32.25%).
Identifikasi Bakteri Escherichia coli dan Salmonella sp. pada Produk Ayam Geprek di Kantin Universitas Tadulako Rofiqoh, Rofiqoh; Kundera, I Nengah; Windarsih, Yulia; Buntu, Amalia; Budiarsa, I Made; Ashari, Abdul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15735

Abstract

This study aims to identify the presence of Escherichia coli and Salmonella sp. in "ayam geprek" products sold at the canteens of Tadulako University. The type of research used is descriptive-exploratory with quantitative examination methods using the Most Probable Number (MPN), Total Plate Count (TPC), and further identification using the Microbact System and Microbact System 2000 Software. Samples were taken from three different faculty canteens using a stratified random sampling technique. The results showed that (1) Test using the Most Probable Number (MPN) method obtained the highest value of 150x102 CFU / ml came from the FMIPA canteen sample, 28x102 CFU / ml samples from the FKIP canteen and the lowest value of 27x102 CFU / ml samples from the FKIP canteen. (2) Test using the Total PlateCount (TPC) method obtained the highest value of 105x103 CFU / ml came from the FMIPA canteen sample, 217x102 CFU / ml FKIP canteen sample, and the lowest value of 120x102 CFU / ml FKIP canteen sample. (3) Validation identification of the percentage of samples showing contamination by Escherichia coli and Salmonella sp., with a validation level above 90%. Thus, all samples in this study were positive for the presence of Escherichia coli and Salmonella sp. bacteria.
Identifikasi Jenis Bakteri Pada Layar Handphone Mahasiswa Pendidikan Biologi dan Pemanfaatannya Sebagai Media Pembelajaran Nabila, Putri; Kundera, I Nengah; Windarsih, Yulia; Sutrisnawati, Sutrisnawati; Budiarsa, I Made; Ashari, Abdul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16094

Abstract

This research aimed to isolate and identify the types of bacteria present on the surface of biology education students' mobile phone screens. This was an exploratory study using a bacterial isolation method.Bacteria found on the mobile phone screens were cultivated using MacConkey agar, a selective medium specifically designed to detect and identify Gram-negative bacteria, particularly Enterobacteriaceae like Escherichia coli. The accuracy of the Escherichia coli data from the phone screen samples, after processing with the Microbact System, was 96.39%.Malt Extract Agar (MEA) was also used as a selective medium to detect and identify Gram-positive fungi, such as Candida albicans.Based on these findings, the developed module was deemed highly feasible. A learning medium is considered highly feasible for use if it achieves a presentation rate between 60-100%. With an average score of 82.39%, the module meets this criterion and can be effectively used in learning.
Hubungan Kekerabatan Kepiting Biola (Uca spp.) di Desa Lalombi Kecamatan Banawa Selatan Kabupaten Donggala Berdasarkan Karakter Morfologi Biung, Yelsan Kanan; Budiarsa, I Made; Trianto, Manap; Kundera, I Nengah; Ashari, Abdul; Jayanti, Zulaikhah Dwi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16380

Abstract

This study aims to analyze the phylogenetic relationships among six species of fiddler crabs (Uca spp.) found in Lalombi Village, Banawa Selatan Subdistrict, Donggala Regency, based on morphological characters. A descriptive-exploratory approach was employed, with purposive sampling to select representative specimens. Eighteen morphometric characters were measured from three individuals of each species: U. triangularis, U. perplexa, U. dussumieri, U. annulipes, U. tetragonon, and U. bellator. Data were analyzed using Principal Component Analysis (PCA) and UPGMA clustering with the aid of PAST software version 4.03. The PCA results revealed that carapace length (CL), anterior carapace width (ACW), and posterior carapace width (PCW) contributed significantly to total morphological variation. Cluster analysis indicated a close phylogenetic relationship between U. tetragonon and U. triangularis, as well as between U. perplexa and U. bellator. U. annulipes appeared intermediate between the two groups, suggesting a moderate level of relatedness. In contrast, U. dussumieri formed a distinct branch, likely due to its markedly larger body size compared to the other species. These findings affirm that morphometric characters are valuable in elucidating phylogenetic relationships within the genus Uca and support taxonomy based on morphological traits.
Co-Authors Abd. Hakim Laenggeng, Abd. Hakim Afiat, Nurul Akram, Akram Amalia Buntu Artha, Fandy Asifah, Nur Astija Bialangi, Mursito S. Biung, Yelsan Kanan Bustamin Bustamin Fatmah Dhafir Febriani, Vita Indri Gamar Binti Non Shamdas Hamsa, Hamsa Hidyanisa, Dhea Hj. Musdalifah Nurdin I Made Budiarsa I Nengah Kundera Jayanti, Zulaikhah Dwi Laksono Trisnantoro Lestari M.P Alibasyah Lilies Lilies Manap Trianto Masriani . Mohammad Jamhari Nabila, Putri Puspitasari, Amira RAFIQA RAFIQA, RAFIQA Rafiqah Rahayu, Irma Septi Rahmadani, Nurlaila Wulan Rizka Fardha rofiqoh rofiqoh Sutrisnawati Mardin Tandi, Jenifa Arisa Windarsih, Yulia