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All Journal Journal of Biotechnology and Natural Science Jurnal Manajemen International Journal of Cell and Biomedical Science Indonesian Journal of Medical and Pharmaceutical Science
Cahyani, Dini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Journal : International Journal of Cell and Biomedical Science

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Temporal Dynamics of TNF-α Expression and Cell Viability in LPS-Stimulated Peripheral Blood Mononuclear Cells Cahyani, Dini; Hidayah, Nurul; Sari, Fikriya Novita
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.49

Abstract

Background: Lipopolysaccharide (LPS), a key component of Gram-negative bacterial membranes, activates innate immune responses through Toll-like receptor 4 (TLR4) signaling in peripheral blood mononuclear cells (PBMCs). This study aimed to evaluate the temporal dynamics of TNF-α expression and cell viability in LPS-stimulated PBMCs to understand the inflammatory and cytotoxic effects of prolonged LPS exposure. Methods: Human PBMCs were treated with increasing concentrations of LPS (10, 30, and 50 ng/mL) for 4, 8, 12, and 24 hours. TNF-α mRNA expression was analyzed using quantitative PCR, while cell viability was assessed via CCK-8 assay and microscopic imaging. Results: LPS stimulation induced a robust, dose-dependent upregulation of TNF-α expression, peaking at 4 hours and gradually declining over time. Concurrently, PBMC viability remained stable up to 12 hours post-stimulation but significantly decreased at 24 hours, particularly at higher LPS concentrations (30–50 ng/mL). Microscopic analysis revealed increased cellular aggregation and morphological changes consistent with immune activation and cytotoxic stress. Conclusion:LPS triggers early TNF-α expression in PBMCs through TLR4-mediated activation of the NF-κB pathway. However, prolonged exposure to LPS results in decreased cell viability, likely due to sustained inflammatory signaling and oxidative stress. These findings provide insight into the dual-phase response of PBMCs to LPS and underscore the importance of tightly regulated inflammation in innate immunity.
hUC-MSCs Therapy with EVs Booster Improves Recovery in Stage 2 Chronic Kidney Disease with Hypercholesterolemia : a case report Jutadi; Ayu, Dian Respati; Cahyani, Dini; Arda, Adzani Gaisani; Anggoro, Naufal Sebastian
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.63

Abstract

Hypercholesterolemia is a common metabolic comorbidity that accelerates the progression of chronic kidney disease (CKD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and their secretome, which consist of extracellular vecicles (EVs) and soluble bioactive molecules, have shown potential in modulating inflammation and metabolism. This case report describes significant improvement in serum lipid profile following hUC-MSC and secretome therapy in a patient with stage 2 CKD and hypercholesterolemia. A male patient with CKD stage 2 received two intravenous cycles of hUC-MSC and secretome therapy administered seven months apart. Serial evaluations demonstrated a progressive decline in total cholesterol from 294 mg/dL at baseline to 286 mg/dL after the first treatment and 225 mg/dL after the second. LDL cholesterol decreased from 188 mg/dL to 140 mg/dL, with a mild rebound to 175 mg/dL. HDL cholesterol, initially elevated at 214 mg/dL, showed a modest increase to 220 mg/dL after the first treatment, followed by normalization to 175 mg/dL. Triglyceride levels remained within the normal range (44–51 mg/dL) throughout the observation period. The marked improvement in lipid parameters suggests that hUC-MSC and secretome therapy may exert systemic metabolic regulation via anti-inflammatory, antioxidative, and hepatoprotective mechanisms. hUC-MSC and secretome administration demonstrated potential benefits in lipid homeostasis in a patient with CKD and hypercholesterolemia. These findings support the role of MSC-derived secretome as a promising adjunctive therapeutic approach. Larger controlled trials are warranted to confirm these outcomes and elucidate underlying mechanisms.
A Novel Regenerative Approach for Acne Vulgaris Using Combined Umbilical Cord Mesenchymal Stem Cell-Derived Secretome and Platelet-Rich Plasma: A Case Report Zamzam, Salsabillah; Kusumaningrum, Novi; Prawitasari, Salindri; Cahyani, Dini; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 4 No 11 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i11.71

Abstract

Background: Acne vulgaris is a chronic inflammatory skin disorder that often leads to scarring and pigmentation. Conventional therapies may provide limited improvement and are frequently associated with adverse effects. Recent advances in regenerative medicine suggest that stem cell–derived secretome and platelet-rich plasma (PRP) may promote skin repair and rejuvenation through anti-inflammatory and regenerative pathways. Case Presentation: A 21-year-old male presented with persistent inflammatory acne and post-acne hyperpigmentation. The patient underwent two sessions of combined SH-MSCs-derived secretome and PRP therapy at two-week intervals. Facial assessments were performed using the Janus Skin Analyzer at baseline, day 14, and day 28. Quantitative analysis revealed significant improvement in multiple parameters, including reduction of pore size, pigmentation index, and sebum levels, accompanied by enhanced skin elasticity. Clinically, the patient exhibited visible improvement in overall skin texture, evenness, and clarity without any adverse reactions. Results: Marked clinical improvement was observed after the second session, with visible reduction in acne lesions, fading of post-acne marks, and overall improvement in skin radiance. Quantitative analysis demonstrated a 30% reduction in pore condition, 58% increase in elasticity, 35% decrease in pigmentation index, and 227% reduction in sebum levels compared to baseline. These findings indicate significant enhancement in skin texture, tone, and elasticity. Conclusion: Combination therapy using SH-MSCs-derived secretome and PRP demonstrated promising regenerative and aesthetic outcomes in this patient with acne vulgaris. Larger-scale clinical studies are warranted to validate efficacy and optimize treatment protocols.
Co-Authors Adityani, Resanti Agung Putra Anggoro, Naufal Sebastian Arda, Adzani Gaisani Ayu, Dian Respati Guntari, Sheirany Ibrahim, Sugeng Jutadi Kresnayanti, Ari Kusumaningrum, Novi Maliza, Rita Noor Rahman, Noor NURUL HIDAYAH Nurwijayanti Prawitasari, Salindri Ricky Firmansyah Rizki, Ivan Muhammad Sari, Fikriya Novita Solihah, Destyani Mariah Yulianty, Neng Novy Zamzam, Salsabillah