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Journal : Journal of Social Research

Escherichia Coli Contamination Test on Thai Tea Drinks in the Ayahanda Street Area Using the MPN Method Dhea F. Mendrofa, Dorkas; Joshua Hamonangan Tua Rajagukguk, Samuel; Suandy, Suandy
Journal of Social Research Vol. 4 No. 10 (2025): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v4i10.2854

Abstract

Thai tea is one of the drinks that are popular with urban people. However, the less hygienic processing and presentation can be a source of microbiological contamination. This study aims to detect the presence of Escherichia coli in Thai tea drinks sold on Jalan Ayahanda, Medan, using the Most Probable Number (MPN) method. The research design was quantitative descriptive with a laboratory experimental approach, using ten samples from Thai tea sellers. The results showed that all samples were positive for coliform with an MPN value of 15–460 MPN/100 mL, eight samples were positive for the BGLB confirmatory test, and one sample (10%) was positive for Escherichia coli in EMB media. Based on the SNI standard (0/100 mL), the positive sample was declared unfit for consumption. These findings confirm the importance of implementing good hygiene and sanitation in the processing and sale of beverages to ensure food safety. They also contribute to the limited body of knowledge on street-level food safety risks in densely populated urban corridors and highlight the urgent need for strengthened street-food hygiene monitoring and consumer awareness to prevent microbial foodborne illness.
Antimicrobial Activity Test of The Combination of Pineapple Leaf Extract (Ananas Comosus) and Green Betel Leaf Extract (Piper Betle L) Against Candida Albicans Buaya, Adjani; Lubis, Yolanda Eliza Putri; Suandy, Suandy
Journal of Social Research Vol. 4 No. 10 (2025): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v4i10.2841

Abstract

Candida albicans is an opportunistic fungus that can cause infections, especially in individuals with low immune systems. Efforts to control this fungal infection can be done by utilizing natural ingredients as an alternative antifungal. Pineapple leaves (Ananas comosus) and green betel leaves (Piper betle L) are known to contain active compounds with antimicrobial properties. This study aims to determine the inhibitory power of a combination of pineapple leaf and green betel leaf extracts on the growth of Candida albicans and to compare their effectiveness at various concentrations. This research is a laboratory experimental study using the disc diffusion method. Three groups of extract concentration combinations were used: 20%, 40%, and 60%. The diameter of the inhibition zone was measured in millimeters, and data were analyzed using a one-way ANOVA test followed by a Tukey HSD test. The results showed that the 20% concentration produced the largest average inhibition zone diameter of 38.32 mm, while the 40% and 60% concentrations produced 28.46 mm and 28.31 mm, respectively. The ANOVA test showed a significant difference between groups (p < 0.05), and the Tukey HSD test showed that the 20% concentration was significantly different from the other two groups. The combination of pineapple leaf and green betel leaf extracts at a concentration of 20% was the most effective concentration in inhibiting the growth of Candida albicans compared to concentrations of 40% and 60%.