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All Journal Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Jurnal Ilmu Ternak Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Ilmiah Ecosystem Jurnal Agrisistem Jurnal Ilmu dan Teknologi Peternakan Terpadu (JITPU) TONGKONAN: Jurnal Pengabdian Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Firmiaty, Sri
Program Studi Peternakan, Fakultas Pertanian, Universitas Bosowa
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Social Sciences Veterinary

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Journal : Jurnal Ilmu Ternak

 1 
The Role of GSH Supplements in Maturation Media in Improving the Bali Cattle Oocyte Fertility by in Vitro Technique Ary, Kana; Idrus, Muhammad; Sonjaya, Herry; Firmiaty, Sri
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 24, No 1 (2024): June
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v24i1.49036

Abstract

In the in vitro oocyte culture process, oxidative stress often occurs, which produces free radicals, so it was necessary to add the antioxidant glutathione (GSH). This research aimed to determine the fertility level of Bali cattle oocytes by adding glutathione (GSH) to the medium maturation. The study was conducted at the In Vitro Embryo Production Laboratory, Institute for Research and Community Service (LPPM) Building, Hasanuddin University in March-May 2023. The study consisted of 4 treatments with the addition of GSH (0 mM, 0.5 mM, 1 mM, and 1.5 mM) with 6 repetitions. The mature oocytes were then subjected to in vitro fertilization. The results showed that the highest fertilization rate (2 PN/Pronucleus) was significantly higher (P <0.05) in the treatment with the addition of 1-1.5 mM GSH to medium maturation, compared to 0.5 mM and without GSH addition, whereas  fragmented oocytes (0 PN), (1PN) and (>2 PN) were not significantly different. This study concluded that adding 1.5 mM glutathione (GSH) to the maturation medium showed the best results, namely 72.26 % fertilized oocytes.
Quality of Bali Cattle Oocytes Post Vitrification with the Addition of Antioxidant Glutathione Sulfihydryl (GSH) in Maturation Media Hidayat, Taufik; firmiaty, sri; syarifuddin, syarifuddin; Sonjaya, h
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 24, No 1 (2024): June
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v24i1.49095

Abstract

The success of oocyte maturation in vitro depends on several factors, namely the type of supplement used in the in vitro maturation medium, the quality of the oocytes used, and the risk of contamination and culture conditions. In vitro culture can cause oxidative stress, generating free radicals that can damage cells, so antioxidants are often added to overcome this problem. This study aimed to determine the effect of adding GSH to the maturation medium on the oocyte quality of Bali cattle after vitrification. This research was conducted at the Emryo In vitro Production Laboratory, Institute for Research and Community Service (LPPM) Building, Hasanuddin University, 2023. The study consisted of 4 GSH treatments: 0 mM, 0.5 mM, 1 mM, and 1.5 mM, with 6 replications. The research variables were viability, fractures of the zona pellucida, cytoplasmic shrinkage, and cytoplasmic lysis in post-vitrification and thawing oocytes. The results showed that adding GSH significantly affected oocyte viability (P<0.05). Oocyte viability in control (without GSH) was significantly lower (P<0.05) compared to the 0.5 mM, 1 mM and 1.5 mm treatments, but between the 0.5 mM, 1 mM and 1.5 mm treatments were not significantly different. The effect of treatment on the fractures of the zona pellucida variables, cytoplasmic shrinkage, and cytoplasmic lysis had no significant effect (P>0.05). It can be concluded that there was an increase in oocyte viability in line with the increase in GSH dose. 
Co-Authors Abdul Latief Toleng, Abdul Latief Abdurrachman Rahim Ahmad Muchlis Ahmad Swandi Akrim, Djusdil Al Hijazi, Alif Fachrur Allo, Relvan Kalla Amiluddin Amiluddin, Amiluddin Andi Rumpang Yusuf Andi Zulfikar Syaiful Andini L, Ayu Ary, Kana Asdar Asdar Asfar, A.M.Fajri Asriani Asser, Andi Bestfy Anitasari Christina Astutiningsih Faidliyah Nilna Minah Fajar, Mohammad Nur Febsrianti Haeruddin Hamsina Hamsina Herry Sonjaya Iskandar, Hikmayani Ismail, Wan Ismahanisa Jafar, Riska Muh. Fikruddin Buraerah Muhammad Ansar, Muhammad Muhammad Basri Muhammad Idrus Muhammad Idrus Ningsih, Dewi Wantika Parmadi, Gio Parwati, Putu Yuni Rande, Hasniar Ambo Rezkyanti, Andi Ririn Suharsanti, Ririn S, Syarifuddin Salim, Andi Randi Sonjaya, h Sudirman Sudirman Sufiyanto, Mohammad Imam Suyadi Suyadi Syarifuddin Syarifuddin Tati Murniati Taufik Hidayat Zainal, Nining Haslinda