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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Klinikal Sains (Jurnal Analis Kesehatan) Jambura Journal of Health Sciences and Research Jurnal Medika Malahayati Husada Mahakam: Jurnal Kesehatan JURNAL ANALIS LABORATORIUM MEDIK Borneo Journal of Medical Laboratory Technology Medicra (Journal of Medical Laboratory Science/Technology) Health Care : Jurnal Kesehatan Jurnal Kesehatan dan Pembangunan Jurnal Kesehatan Tambusai Jurnal Sains dan Kesehatan Borneo Journal of Science and Mathematics Education Journal of Sustainability Science and Technology Gema Kesehatan
Kesuma, Suryanata
Poltekkes Kemenkes Kalimantan Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Journal : THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

 1 
Evaluasi Analitik Hematology Analyzer Diatron Abacus 3 Pada Parameter Hematologi Rutin Di Laboratorium Hematologi Poltekkes Kemenkes Kalimantan Timur Suryanata Kesuma; Mahdiah Syumarliyanty; Agus Rudi Hartono
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.6467

Abstract

Entering the era of globalization, manual tools in clinical laboratories have been replaced by full automatic devices. One of them is the Diatron Abacus Hematology analyzer 3. A relatively new hematology analyzer is required for analytical evaluation. Analytical evaluation is an evaluation of Diatron Abacus 3 on Sysmex KX 21 as a standard in RSUD I.A Moeis Samarinda, and it is very important to do this to assess the performance of the tool. Analytical evaluation is done by determining the value of accuracy, precision, and total error and linearity of measurement results from routine hematological examination parameters, which are erythrocytes, leukocytes, platelets, hemoglobin, and hematocrit. To determine the results of the hematology analyzer evaluation of Abacus 3 Diatron Analyzers on Routine Hematology Parameters in the Hematology Laboratory of the Health Ministry of Health, East Kalimantan. This type of research is observational descriptive, using a total sampling technique, and a sample of 40 complete K3EDTA blood specimens. Data processing using Microsoft Excel and SPSS 20 applications, analyzed using descriptive statistics. The observations were still included in the criteria for acceptance, accuracy or inaccuracy (d%) in erythrocytes 1.8%, leukocytes 8.0%, platelets -5.3%, hemoglobin 2.3% and hematocrit -1.7%; Precision or impression (CV%) in erythrocytes 4.2%, leukocytes 11.1%, platelets 6%, hemoglobin 3.9% and hematocrit 4.5%; Total errors in erythrocytes were 8.7%, leukocytes 17.9%, platelets 23.6%, hemoglobin 8.8% and hematocrit 9.1; Linearity of the measurement results against the routine hematological cell count values performed using Abacus 3 and Sysmex KX 21 has a positive relationship. Acceptance values are still included in the LOA on all parameters examined and still meet the criteria; the accuracy/bias value is smaller than the true value of the parameter being examined, except for leukocytes, which is greater; the precision on the five parameters is greater than the CV% Abacus 3 insert kit; The total error obtained by the TE value is greater than the TEA in the parameters examined, except for smaller platelets; Linearity of the measurement results against the calculated hematology cell routine values performed using Abacus 3 and Sysmex KX 21 have a positive relationship, meaning an increase in measurement values using Abacus 3 is followed by an increase in measurement values using Sysmex KX 21.
Evaluasi Penggunaan Biosaliva Dalam Deteksi Sars-Cov-2 Metode RT-PCR Suryanata Kesuma; Suparno Putera Makkadafi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11280

Abstract

SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection.
Co-Authors Adi Firmansyah Agus Rudi Hartono Agus Rudi Hartono Amalia Diah Suci Rahmah Anggrieni, Nurul Armiah Gita Anggraini DESI WAHYUNI Didi Irwadi Dwi Setiyo Prihandono Dwi Wulan Sari Effie Raissa Widyadari Eka Farpina Eka Farpina, Eka Farpina Elita Octavia Farhan Baehaki I Gede Andika Sukarya Irawiraman, Hadi Lamri Lamri Lamri Lamri Liah Kodariah Mahdiah Syumarliyanty Marezha Aulia Putri Sugiarsono Maulida Maulida Julia Saputri Nanda Nur Adha Sari Febriyanti Nashi Widodo Nia Ardelia Nursalinda Kusumawati Nurul Alidasyah Nurul Hikmah Oktavia Rahayu Adianingsih Patricia Aleksandra Putri Sekar Sari Rahmad Rezaldy Silvi Marshela Siti Rodiyah Sresta Azahra Sri Winarsih Sultan Sultan Sultan Sultan Suparno Putera Makkadafi Suparno Putera Makkadafi Supri Hartini Tatang Abdullah VALENTINA YURINA