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Journal : Jurnal Ilmu Ternak Veteriner

Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products Merryafinola Ifani; Efka Aris Rimbawanto; Fransisca Maria Suhartati
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2829

Abstract

The study was aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro based on a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (T0); 1.5% (T1); 3% (T2); and 4.5% (T3). Data obtained were analyzed by analysis of variance and tested using orthogonal polynomials. Results showed that  addition of protected soybean meal with mahogany leaf extract had a cubical effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effecton N-NH3, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.
Rumen Fermentation Profiles of Protein-Energy Synchronization Index-Based Ration: an In Vitro Study Afduha Nurus Syamsi; Merryafinola Ifani
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 1 (2023): March 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i1.3091

Abstract

The study examined the effect of protein-energy synchronization (PES) index-based rations on the rumen fermentation profile. The material used was the Jawa Randu goat's rumen fluid, collected soon after the goat was slaughtered. The treatment ration consisted of elephant grass, lamtoro (Leucaena leucocephala), coconut meal, tofu waste, bran, cassava waste, and mineral mix arranged based on the PES index of each feed ingredient. The research was conducted in vitro using a completely randomized design (CRD). The treatment consisted of 4 PES indexes, namely 0.55 (R1); 0.6 (R2); 0.65 (R3); 0.7 (R4), and each had 5 replications. Data were analyzed by ANOVA and orthogonal polynomials (OP). The results of the ANOVA showed that the PES index did not affect the production of acetate (C2) and butyrate (C4) but had a highly significant effect on dry matter digestibility (DMD), organic matter digestibility (OMD), pH, a total of volatile fatty acids (VFA), propionate (C3), C2:C3 ratio, methane (CH4), ammonia (NH3), and microbial protein synthesis (MPS). The analysis of OP showed a quadratic effect on all variables with the equation Y= - 81.601X2 + 375.04X – 310.78  (DMD), Y= -433.69X2 + 522.69X – 128.75 (OMD), Y=-44X2 + 54.04X – 9.9 (pH), Y= -2160X2 + 2576.8X – 604.2 (VFA Total), Y= -481.8X2 + 585.01X – 143.45 (C3), Y = 50.93X2 – 60.177X + 21.067 (C2:C3), Y = 202.45X2 – 223.18X + 103.41 (CH4), Y= 436X2 – 552.28X + 181.08 (NH3), dan Y= -1012X2 + 1260X – 311.64 (MPS). Protein-energy synchronization (PES) index of 0.6 achieved the best rumen fermentability. The formulation of the PES index-based ration of 0.6 had the most effective compared to other indexes based on the high of propionate, the lowest methane, the lowest NH3, and the best microbial protein synthesis. The ration with an index of 0.6 is composed of 30% elephant grass, 30% Leucaena leucocephala, 10% coconut meal, 10% tofu waste, 10% rice brand, 9% cassava waste, and 1% mineral mix.