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Journal : Eruditio : Indonesia Journal of Food and Drug Safety

 1 
Penetapan Limit of Detection (LOD) dan Sensitivitas Metode Deteksi Pseudomonas aeruginosa dalam Berbagai Matriks Sediaan Obat Nur Aini; Maria Purba; Sitoresmi Triwibowo; Nenden Sholihatul; Ratna Wulandari
Eruditio : Indonesia Journal of Food and Drug Safety Vol 1 No 2 (2021): Edisi Juni
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.059 KB) | DOI: 10.54384/eruditio.v1i2.85

Abstract

Contamination of certain microorganisms in pharmaceutical products can potentially reduce or even inactivate therapeutic activity and harm patient health. Pharmacopoeia monograph determine the microbiological quality in medicinal products. One of them is P. aeruginosa parameters which should not be present in pharmaceutical preparations. Pharmacopoeia states that microbial detection methods must be able to detect microorganisms, including P.aeruginosa not more than 100 colonies, but the actual detection limit has never been set quantitatively and many variables can affect the microorganism recovery. This study aims to determine the range of Limit of Detection (LOD) values and the sensitivity of the P. aeruginosa method on pharmaceutical products. Detection method of P. aeruginosa refers to the specific microbial test of the Indonesian Pharmacopoeia VI (2020) is culture media methods. Experiments used eight pharmaceutical products representing five dosage forms contaminated with P. aeruginosa ATCC 9027 with three levels of concentration. The results determined LOD range and sensitivity of P. aeruginosa detection method in pharmaceutical products, and analyzed descriptively. This study shows that pharmacopeia method of P. aeruginosa testing can detect P. aeruginosa in various drug preparation matrices with a detection limit of 1-10 colonies and a sensitivity of 100%. Results of this study can be used as reference for LOD value in the verification of the P. aeruginosa detection in medicinal product.
Deteksi Salmonella spp pada pakan hewan laboratorium berbentuk pelet menggunakan Real Time Polymerase Chain Reaction (qPCR) dan Loop Mediated Isothermal Amplification (LAMP) P, Fitra Yovita Delviona; Puspita Dewi Fortuna; Nur Aini
Eruditio : Indonesia Journal of Food and Drug Safety Vol 5 No 1 (2024): Edisi Desember
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54384/eruditio.v5i1.194

Abstract

Salmonella spp. contamination in animal feed is challenging for the commercial feed industry and laboratory animal facilities. Salmonellosis is a term used for Salmonella infection. Salmonellosis is a zoonosis directly transmitted to humans through contaminated feed, food, water, or infected animals. Nevertheless, infection in animals is mainly caused by contaminated feed. Since it provokes a significantly high economic loss due to its high mortality, accurate and rapid Salmonella detection methods are necessary for monitoring the quality of animal feed. The standard method for Salmonella detection in animal feed, which refers to ISO 6579, is based on the culture method, which takes about 5-7 days. Thus, an alternative method is required to give valid results faster. This study aims to develop alternative methods for Salmonella spp. detection in animal feed using Loop isothermal Amplification (LAMP) and Real-time Polymerase Chain Reaction (PCR) methods. Salmonella detection has been carried out on animal feed pellets artificially inoculated with Salmonella Typhimurium ATCC 14028 at three concentration levels, namely 1, 3, and 9 cfu per test portion (25-gram samples). e-LOD range values between the LAMP method and Real PCR are compared with the culture method and analyzed descriptively. Salmonella detection method using LAMP and Real-Time PCR showed the value e-LOD50 at 0.62 cfu/portion in mouse feed and e-LOD50 at 0.37 cfu/portion in rabbit feed. Compared to the LOD of the culture method, the e-LOD of LAMP and PCR showed similarity. This e-LOD value is the same as the e-LOD culture method. Related laboratories can utilize the results of this study to monitor the quality of animal feed.
Co-Authors Aas Aisyiah Abdul Halim Abdul Jabpar Abdul Kodir Djaelani Abdul Muad Ade Irma Purnama Sari Ade Rahmi Nasrida Afandy Muharrom Affiq faeyza Afriyadi Afriyadi Ahmad Alfa Rizi Sitompul AHMAD HULAIMI, AHMAD Ahmad Munajat Ahmad Wafi Ainul Mahfud Ainun Adilah Dly Ainun Hidayasha Akmal Saleh Aldi Wijaya Aleria Irma Hatneny Ali Jusri Pohan Alinda Alya Nabila Alyah Khairani Ananda Febriliana Cantika Anita Deborah Anwar Anjaliyatul Luaily Anndy Prastya Annisa Cahya Kusumawati Apri Cahyani, Fatkha Aqnes Budiarti Ari Suriani Arifiyanto Hadi Negoro Aris Munandar Arnold Sihombing Aspan Martua Asriwati Asriwati Astri Astuti Audia Amanda Br Tarigan Aulia Mei Sandrina Bachtiar Hariadi Bahasa Indonesia Bambang Setyo Berliana Sari Harahap Bernarda Krinadadita Brema Heva Faralita Brigita Winda Sari Danial Khaled Syamna Davito Bagus Hernandhi Dede Alfiyah Nurhovivah Dedi . Deli Theo Delmira Syafrini Devi Aprianti Devi Nisa Hidayati Dewi Arlifah Cahyani Dewi Pusparini Dhea Arsita Dhea Rizky Istiqomah dian oktaviani Dian Purnomo Jati Dian Purnomojati Dluha Maf’ula Dr.H. Arif Rembang Supu Dwi Dini Farah Diva Dwi Lestari Ega Jati Damara Elsa Phelia Elvina Tabuni Endro Pramono Erma Wahyu Mashfufa Fara Brygita Ramadhani Fhadia Nur Baiti Finny Ligery Firman Agung Pradana Gina Elfida H. Muhammad Imam Syafi'I H. Tengku Darmansah Habib Nabawi Hanif Amrulloh Hanifa Nurhamidin Herri S. Sastramihardja Heru Nugroho Hidayat Arifin Hidayat Wijayanegara Ida Busnetty Ifrohatul Hasanah Ika Rahmawati Ilda Alini Hasibuan Ilma Farida Inda Lestari Indah Anggraini Indra Lila Kusuma Inom Nasution  INTAN AMALIA Iriani Ismail Irma Suwarning Irma Valentina Manurung Iskandar Zulkarnain Ismail Efendy Istiqomah Iwan Setiawan Iwanussholeh Iwanussholeh Jaenullah Jaenullah, Jaenullah Janitra, Fitria Endah Joko Dwi Santoso Juarisman Juliandi Harahap Junaidi Junaidi Keisha Putri Dwiyan Khoirunnisa Nasution Kholidaziah, Kholidaziah Kurnia Aldiba Kustanti, Christina Yeni Kusuma Wardana, Raka LAILATUL FITRIYAH Lathiefa Rusli Lili Purnama LILIS SETYOWATI Linawati Ningsih Lismawati, Lismawati Lusiana Winda H.K Lusy Rahmawati M. Afif Rijal Husni M. Asy’ari M. Iqbal Syarifuddin M. Khalid Pay Hasibuan M. Kharis Fadillah M. Naufal Muhadzib Al-Faruq M. Sudirman Mappeaty Nyorong Mardiyah Maria Meisien Maria Purba Marzicha Amalia Sumardiko Maslichah, Maslichah Masruhen Mawaddah Mawaddah, Mawaddah Mawar Sri Puji Efendi May Hasanah Maya Tsurroya Alfadla Maylizza Putri Dyansyah Ma’mun Sutisna Melati Fajarini Meti Widiya Lestari Mhd Ikhsan Ibrahim Miftachudin Miskah Afriany Mochammad Isa Ansori Mohammad Muslih Muhammad Farhan Rizqy Muhammad Ivan Noor Salim Muhammad Lutfi Arafandi Muhammad Ravito Muhammad Rizky Muhammad Rizky Ramadhani Mutia Puspita Sari Nadia Royani Nst Nadiah Najah Nadya Isra Miranti Nasim Nasim Nasrullah Hidayat Nasution, Ramadhani Syafitri Nazwa Dwi Fahira Nenden Sholihatul Niken Rahmanti NINIK TRI HARTANTI Nonik Kasiari Novia Ariani Nur Aida Nur Hafikrah Lubis Nur Halimah Lbs Nur Ila Ifawati Nur Laili Nur Sakinah Siagian Nurani Lubis Nuri Cahyono Nuringsih, Nuringsih Nurma Tambunan Nurmala Nurmala NURSUPIAN NURSUPIAN Nurul Apriatun Nurul Fitriyah Nurul Hidayatus S Ollyvia Freeska Dwi Marta Ollyvia Freeska Dwi Matra Oman Rusmana P, Fitra Yovita Delviona Panji Ratih Suci Paquita, Paquita Al Husna Pipit Festy Wiliarnarti Pujawulan Dari Puspita Dewi Fortuna Putri Nurdiana Putri, Alifiani Rahmi QURROTA A’YUN R. Taufiqurrochman Rachmy Aulia Rahmi Rifalina Ramadani Fitriyah Harahap Ramadhani Syafitri Nasution Randi Hendrizal Ratna Wulandari Ratna Yulika Go Razia Begum Suroyo Riendza Wibowo Rika Anggraini Rindang Diannita Rini Lestari Rio Setiawan Risda Sari Riska Pristiani RISKIA Rizki Cahya Wahyuni Rizky Agus Rinaldi Rosalinda Rosalinda Rukiah Santi Asria Lubis Sari Inrianita Sarifa Sarifa Sartika Hutasuhut Selvi Damayanti Setyono, Vicky Indarto Sevia Diah Oktaviana Shalomita Agustina Sindi Pertamasari Sirajuddin Sirajuddin Siti Maulidur Rohma Siti Rodiah Lubis Siti Roudhatul Jannah Siti Shofiyah Siti Wulandari Sitoresmi Triwibowo Sofyan Ari Saputra Sri Hayati Sri Solihatun Stefani Karina Syafitri Suaidatul Mufidah Suci Canda Ati Suharjono Sulistyawati Sultan Salahudin Sunardi Sunariyo, Najwa Suprihatin Suprihatin suryaningsih Syafira Maharany Syamsuyurnita, Syamsuyurnita Syawaluddin Syawaluddin Syilvana Rohmawati Dewi Syofiarti, Syofiarti Syujana Ekapardas Tatiya Wulandari Teguh Aqilah Ramadani Tengku Darmansah Tengku Sinar Marwanda Thomson Parluhutan Thomson Parluhutan Nadapdap, Thomson Parluhutan Tiara Octary Titin Fatimah Tiya Purnama Sari Toton Fanshurna Tri Niswati Utami Triani Arofah Try Aisyah Rani Purba Ummu Khoiriah Rangkuti Umratul Hasanatun Uyock Anggoro Saputro Vania Angel Veni Edrian Wahyu Ernawan Wahyu Hidayat Wahyu Nurul Rosiana Wawan Kurniawan Wildan Rahmawan Wita Ramadhanti Yani Sofiani Yarzan Manukalia Yuanita Wulandari Yuniati Yuniati Yunitri, Ninik Yuntari Purbasari Yusna Alawiyah Yusri Yusri Zaehol Fatah Zihan Saputra Zulfikar Lubis