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All Journal UNEJ e-Proceeding Nusantara Medical Science Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Ilmu Alam dan Lingkungan Indonesian Journal of Obstetrics & Gynecology Science Alami Journal (Alauddin Islamic Medical) Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Biomedika Jurnal Riset Kesehatan Poltekkes Depkes Bandung Celebes Health Journal Indian Journal of Forensic Medicine & Toxicology JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Majalah Anestesia & Critical Care (MACC)
Firdaus Hamid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Jurnal Biomedika

 1 
Detection of LytA Genes in Streptococcus pneumoniae Isolated from sputum pneumonia patients Mustika Sari Hutabarat; Firdaus Hamid; Irawaty Djaharuddin; Alfian Zainuddin; Rossana Agus; Muhammad Nassrum Massi
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.718

Abstract

Streptococcus pneumoniae (pneumococcus) is a Gram-positive facultative anaerobic bacterium that is a major cause of morbidity and mortality worldwide. But the lack of reporting of disease by this bacterium in Indonesia, one of the causes is because the diagnosis of pneumococcal infection is often clinically not typical and conventional methods which are still the standard gold method often give false-negative results. So the purpose of this study was to evaluate the performance of culture and molecular diagnostic methods using the Polymerase Chain Reaction (PCR) technique in detecting Streptococcus pneumoniae in sputum clinical samples using the Autolysin (LytA) gene which is a virulence factor of this bacterium. 57 isolates from 60 samples were confirmed as Streptococcus sp through microscopic identification, culture, and biochemical tests. Then the sensitivity test with an optochin test of 9 (9%) compared the results descriptively with the PCR technique using the Autolysin A (LytA) gene which was obtained more sensitive by 15 (25%).
Prevalence of Listeria spp. with PRS Genes in Fresh Vegetables from Traditional Markets A. Kaisar Adiwijaya Putra; Rizalinda Sjahril; Arif Santoso; Dianawaty Amiruddin; Andi Alfian Zainuddin; Firdaus Hamid
Biomedika Vol 13 No 2 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i2.842

Abstract

Listeriosis is a foodborne infectious disease caused by Listeria monocytogenes and is considered a serious health problem, due to the severity of symptoms and a high mortality rate in worldwide This study aims to identify and determine the prevalence of Listeria species through prs gene screening of fresh vegetables distributed in several markets of Makassar City. A total of 57 fresh vegetable samples were collected from February to May 2019 in four traditional markets Makassar. The isolates is examines to phenotypically and genotypically Vitek and Multiplex PCR with prs and lmo1030 primer. Phenotype analysis did not show the presence of Listeria species, but the results of genotypic was found 8 positive prs gene samples (14.03%), consisting of 31.2% long beans, 18.2% cabbage, and 9.1% cucumber and. All Listeria species found in this study is Listeria monocytogenes. This study also provide information and additional data that prs genes can be used as screening genotype for identify Listeria species in fresh vegetables.
The Analysis of Vitamin D Receptor Protein on Salmonella typhi infection in acute recurrent cases in endemic area in Eastern Indonesia Fatmawati Annisa Syamsuddin; Mochammad Hatta; Firdaus Hamid; Rosdiana Natzir; Ahyar Ahmad; Burhanuddin Bahar
Biomedika Vol 14 No 2 (2021): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v14i2.1298

Abstract

The host susceptibility mechanisms such as Vitamin D Receptor (VDR) is involved in the modulation of macrophage function and may possibly correlate with immunity disease including the severity of typhoid fever symptoms. The study aimed to assess the VDR Protein expression in the serums of recurrent acute typhoid fever (RATF) patients and compares it with typhoid fever (TF) patients, and healthy persons (HP). The study employed 30 RATF patients and 30 TF patients selected from primary health centres and hospitals in Eastern Indonesia as the endemic area. All the samples were obtained from several health centers in South Sulawesi, Southeast Sulawesi, Central Sulawesi, East Kalimantan and Papua and then collected in the sample bank Biology Laboratory of Molecular Immunology, Faculty of Medicine, Hasanuddin University. As a comparison, 30 samples of healthy persons were also selected from the Blood Transfusion Unit in Makassar, South Sulwesi Indonesia. The profile of VDR Protein was analyzed with Enzyme-linked Immunosorbent Assay (ELISA). VDR protein content data on RATF and TF were designed according to completely randomized design T test. Subsequently, it correlated to Pearson correlation to determine the interaction between Widal titre and VDR protein levels. A comparison between Widal titre and VDR Protein level was also made to identify the correlation. It was found that the mean of VDR protein expression of RATF was 13,44 ng/mL, the mean of VDR protein expression of TF was 24,88 ng/mL, and the mean of VDR protein expression of HP was 43,49 ng/mL. The correlation results between RATF-TF Widal titre and VDR protein level indicated a negative correlation with p-value of 0,004. There were significant differences in the VDR expression in the RATF, TF, and HP. RATF VDR expression lower than TF and HP and there was also a correlation between Widal titre with VDR Protein expression.
Co-Authors A. Kaisar Adiwijaya Putra A.M. Takdir Musba Aan Yulianingsih Aan Yulianingsih Anwar Ade Rifka Junita Agussalim Bukhari Ahyar Ahmad Ahyar Ahmad Alamsyah Ambo Ala Husain Alfian Zainuddin Alfina Baharuddin Aminuddin Aminuddin Andi Alfian Zainuddin Andi Alfian Zainuddin Andi Asadul Islam Andi Ihwan Andi Meutiah Ilhamjaya Andi Nursanty Padjalangi Andi Rofian Sultan Andi Zsazsa Rafiatul Mukhlis Arif Santoso Arif Santoso Arsyi Adliah Anwar Awaluddin Awaluddin Baedah Madjid Besse Afriana Arif Bukhari, Agussalim Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Dario A Nelwan Dianawaty Amiruddin Eddy Hartono Eddyman W Ferial Efendi Lukas Elizabeth Catherine Jusuf Erwin Chandra Evi Damayanti Faridha Ilyas Fatmawati Annisa Syamsuddin Febyan Rasmin Kotto Fonny Josh Hardjono Sumarlie Hursepuny, Valentine Irawati Djaharuddin Irawaty Djaharuddin Junus Asiu Bulu Baan Kenny Edward Yap Khairuddin Djawad Lisa Tenriesa M. Sabir Madonna Damayanthie Datu Misriyani Misriyani Mochammad Hatta Mochammad Hatta Mochammad Hatta Muh. Nasrum Massi Muh. Ramli Ahmad Muhammad Nassrum Massi Muhammad Reza Primaguna Mustika Hutabarat Sari Mustika Sari Hutabarat Nasrudin Andi Mappeware Nita Mariana Noor Ramadhaniah Novita Prastiwi Nur Indah Purnamasari Nur Surya Wirawan Nusratuddin Abdullah Priady Wira Prasetia Ressy Dwiyanti Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rosdiana Natzir Rossana Agus Sachraswaty Rachman Samrichard Rambulangi Sharvianty Arifuddin Siska Nuryanti Siti Fitrah Irmiyanti Hamid Solly Aryza Sri Wahyu Sri Wahyuni Sulmiati Sulmiati Suryani As’ad Syahruni Syahrir TOKA, WAHYUNITA DO Veni Hadju Wahyuni Alinengsih Yoeke Dewi Rasita Yulfa Rizki Amita