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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Buletin Peternakan International Journal of Science and Engineering (IJSE) Jurnal Ilmu Ternak Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture agriTECH ZOO INDONESIA Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Jurnal Aplikasi Teknologi Pangan Tropical Animal Science Journal Indonesian Food and Nutrition Progress Jurnal Pertanian Agros JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Aplikasi Teknologi Pangan MANAZHIM Jurnal Ilmu Peternakan Terapan Buletin Profesi Insinyur Journal of Tropical Animal Science and Technology Jurnal Sains dan Teknologi Peternakan Zoo Indonesia
Zuprizal (Zuprizal)
Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Veterinary Other

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Journal : Jurnal Aplikasi Teknologi Pangan

 1 
Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi Cahya Setya Utama; Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Indonesian Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jatp.2155

Abstract

Penelitian bertujuan mengisolasi dan identifikasi jenis bakteri asam laktat (BAL) selulolitik yang berasal dari jus kubis terfermentasi. Tahapan penelitian meliputi isolasi mikrobia dari jus kubis terfermentasi, karakteristik morfologi sel, karakteristik biokimiawi, identifikasi dengan kit api 50 CHL dan pengujian kemampuan mendegradasi selulosa. Penelitian diawali dengan memfermentasi jus kubis selama 6 hari pada kondisi anaerobic fakultatif. Hasil fermentasi diisolasi dengan media de man rogosa and sharpe (MRS) yang ditambahkan CaCO3 1% dan diinkubasi selama 24 jam. Reinokulasi dilakukan sebanyak 5 kali sampai ditemukan kultur murni BAL, kemudian diidentifikasi dengan menggunakan kit analytical profile index (API) 50 CHL. Hasil identifikasi kemudian diuji kemampuannya untuk mendegradasi selulosa pada media MRS yang ditambahkan 1% carboxymethyl cellulosa (CMC) dan 0,1% congored sebagai indikator dan diinkubasi selama 24 jam. Hasil penelitian menunjukkan bahwa strain bakteri yang teridentifikasi adalah Lactobacillus plantarum dan Lactobacillus brevis yang dapat mendegradasi selulosa. Kesimpulan dari penelitian ini adalah ditemukannya 2 jenis bakteri asam laktat dari jus kubis terfermentasi yaitu Lactobacillus plantarum dan Lactobacillus brevis yang mempunyai sifat selulolitik.Isolation and Identification of Lactic Acid Bacteria Cellulolitik Originated from Fermented Cabbage JuiceAbstractThe objective of this study was to isolate and identify the type of lactic acid bacteria (BAL) of cellulolytic originating from fermented cabbage juice. Research was conducted with the following steps: microbial isolation from fermented cabbage juice, cell morphology characteristics, biochemical characteristics, identification with an analytical profile index (API) 50 CHL kit, and cellulose degradability testing. The research was begun by fermenting cabbage juice for 6 days under facultative aerobic condition. The fermentation product was then isolated with de man rogosa and sharpe (MRS) medium which added CaCO3 1% and incubated for 24 hours. Reinoculation was performed 5 times until BAL was found. BAL pure cultures were used to identify lactic acid bacteria strains using an analytical profile index (API) 50 CHL kit. After pure culture was found, it was then tested for the ability to degrade cellulose on MRS media with 1% carboxymethyl cellulose (CMC) and 0.1% congored as indicator and incubated for 24 hours. The results showed that the identified bacterial strains were Lactobacillus plantarum and Lactobacillus brevis which could degrade cellulose. The conclusion of this research was the discovery of two types of lactic acid bacteria from fermented cabbage juice namely Lactobacillus plantarum and Lactobacillus brevis which had cellulolytic properties.
Co-Authors . Supadmo A. Darwoko A. Kurniawati A. M. Chagneau A. Subhan A.S. Nugraha Agus Hadi Prayitno Ali Agus Ali Mas'adi Ali Mursyid W. M. Ali Mursyid Wahyu Mulyono Amrullah, Yusuf Amri Andri Kusmayadi Ari Kusuma Wati Arif Ismail Ariyadi, B. Astriana Napirah B. Ariyadi B. Ariyadi Bambang Ariyadi Bambang Ariyadi Baskara, A. P. Baskara, Aji Praba Besse Mahbuba We Tenri Gading C. Hanim Cahya Setya Utama Cahya Setya Utama Chusnul Hanim Cuk Tri Noviandi D Hardini Daniel Happy Putra Dina Oktaviana Dini Hardini Dini Hardini Dini Julia Sari Siregar Dwi Sulistiawati E. Indarto E. Suryanto E. Tugiyanti Edi Suryanto Edwin Indarto Elly Tugiyanti Eni Siti Rohaeni Eny Sholikhatin Fatima Indah Hayati Ferry Poernama Ferry Poernama H. Sasongko Hardi Julendra Hari Eko Irianto Harimurti Februari Trisiwi Harwi Kusnadi Heni Suryani Heru Ponco Wardono Hery Widijanto Hestu Widyatmoko Hesty Natali Himmatul Khasanah I. Widiyono Indratiningsih (Indratiningsih) Ismoyowati ISTNA MANGISAH Jafendi Hasoloan Purba Sidadolog Jamhari (Jamhari) K. Sholiha Khusnan Khusnan Kusmayadi, A. Kustantinah Kustantinah Kustantinah Kustantinah Kustantinah Lisnahan, Charles V. M. Hidayat M. Kamal M. Larbier M. Nur Cahyanto M. Nur-Cahyanto Muhammad Dani Muhammad Dani Muhammad Hidayat Muhammad Nur Cahyanto N. A. Hidayati N. D. Dono N. D. Dono N. D. Dono N. Ningsih Nanung Danar Dono Nasroedin (Nasroedin) Niati Ningsih Niati Ningsih Niati Ningsih Ning Iriyanti Ning Iriyanti Nur Widodo Nuryono Nuryono R Martien, R R. E. Indarto R. Edwin Indarto R. Martien Rateh Wulandari Ratih Dewanti Riswantiyah (Riswantiyah) Rr. Retno Widyani Rusman Rusman Rusman Rusman Rusman Rusman S Keman, S S. Kismiati S. Kismiati S. Sundari S. Supadmo S. Supadmo Setyono, W. Singgih Hawibowo Sinta Maharani Sinta Maharani Siska Fitria Siska Fitria Siti Zubaidah Soeharto Prawirokusuma Soenarjo Keman SOEPADMO SOEPADMO, SOEPADMO Sri Harimurti Sri Harimurti Sri Purwanti Sri Sudaryati Sunarjo Keman Sundari Sundari Supadmo (Supadmo) Supadmo . Supadmo Supadmo Supadmo Supadmo Supadmo Supadmo Syamsul Arifin Sylvia C. H. Hehanussa T Yuwanta T. Yuwanta T. Yuwanto Taufik Ahmad Zulfian Taufik Ahmad Zulfian Theresia Nur Indah Koni Tri Yuwanta Tri Yuwanta Trijoko (Trijoko) U. Atmomarsono U. Maesaroh Umar Santoso Umi Maesaroh Veithzal Rivai Zainal W.M. Ali-Mursyid Wati, A. K. Wati, Ari Kusuma Wihandoyo (Wihandoyo) Wihandoyo , Wihandoyo Wihandoyo Wihandoyo Wihandoyo, Wihandoyo Wihandoyo, Wihandoyo YUNIANTA YUNIANTA Z. Bachruddin Zaenal Bachruddin Zubaidah, S. Zulfa, I. H.