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Muhammad Rais
Universitas Negeri Makassar
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Journal : Jurnal Teknologi Pertanian

 1 
ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PADA FERMENTASI SPONTAN BIJI KOPI ROBUSTA ASAL BANTAENG Izmi Azhara; Muhammad Rais; Andi Sukainah; Reski Praja Putra
Jurnal Teknologi Pertanian Vol. 23 No. 1 (2022)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1098.007 KB) | DOI: 10.21776/ub.jtp.2022.023.01.5

Abstract

          Penelitian ini adalah penelitian eksperimen yang bertujuan mengisolasi bakteri asam laktat (BAL) indigenus yang terlibat dalam fermentasi spontan biji kopi robusta asal Bantaeng pada interval waktu fermentasi 0, 3, 6, 9, 24, 48, dan 72 jam serta untuk mengetahui aktivitas enzim dari isolat bakteri asam laktat indigenus sebagai upaya pengembangan kultur starter dalam fermentasi biji kopi secara terkontrol. Hasil penelitian menunjukkan isolasi berhasil dilakukan pada 37 isolat bakteri yang terlibat dalam fermentasi spontan biji kopi robusta. Hasil identifikasi sederhana yang dilakukan secara bertahap menunjukkan hanya 12 isolat bakteri yang bersifat katalase negatif, Gram positif, non spora, non motil, tidak menghasilkan gas (homofermentatif), tumbuh pada suhu 10 °C, 37 °C, 45 °C serta mampu tumbuh pada konsentrasi garam 4% dan 6,5%. Selanjutnya, 2 isolat BAL yang diisolasi pada waktu fermentasi 24 jam diuji lebih lanjut untuk dikembangkan sebagai kultur starter fermentasi biji kopi secara terkontrol. Kedua isolat BAL terpilih, yaitu BT24I1 dan BT24I2, diuji kemampuannya dalam menguraikan selulosa dan pektin. Hasil pengujian kualitatif menunjukkan kedua isolat BAL mampu menghasilkan selulase dan pektinase sehingga keduanya berpotensi dikembangkan sebagai kandidat kultur starter dalam fermentasi terkontrol biji kopi robusta dalam upaya pengembangan mutu dan cita rasa kopi                                                                 This study is an experimental study aimed at isolating lactic acid bacteria (BAL) indigenus involved in the spontaneous fermentation of robusta coffee beans from Bantaeng at fermentation time intervals of 0, 3, 6, 9, 24, 48, 72 hours and to find out the enzyme activity of the isolate of indigenus lactic acid bacteria as an effort to develop a starter culture in controlled coffee bean fermentation. The results showed that isolation was successfully carried out on 37 bacterial isolates involved in spontaneous fermentation of robusta coffee beans. The results of a simple identification carried out gradually showed only 12 bacterial isolates that were catalase negative, Gram positive, non spore, non-motile, non-gas-producing (homofermentative), growing at temperatures of 10 °C, 37 °C, 45 °C and able to grow at salt concentrations of 4% and 6.5%. Furthermore, 2 BAL isolates isolated at the fermentation time of 24 hours were further tested to be developed as a controlled coffee bean fermentation starter culture. The two selected BAL isolates, BT24I1 and BT24I2, tested their ability to decompose cellulose and pectin. Qualitative test results show that both BAL isolates can produce cellulase and pectinase so that both have the potential to be developed as candidates for starter cultures in controlled fermentation of robusta coffee beans in an effort to develop the quality and taste of coffee.
Co-Authors A.A. Ketut Agung Cahyawan W Adan, Laode Muhammad Hasrul Adan Agusprianti, Agusprianti Agustiawan, Agustiawan Agustyawati, Dwi ahmad, astrid Akbarsyah, Muhammad Aksar, Attariq Dihqan Faza Alamsyah, Andi AMINUDDIN, ROSIHAN Aminuyati Amirah Mustarin Andi Nur Putri Andi Sukainah Andi Sukainah Andriani Andriani Arifyansah, Rahmat Ariyanti, Kadek Dwi Arruan, Yernalia Dendang, Nataniel Deswita, Santi Dwi Hasriani, Raden Emi Mastura Eriawati Eriawati Fadilah, Ratnawaty Fadillah, Ratnawaty Farida Aryani Farida Aryani Fauziah Hayat, Riska Febrian, Andis Febrianto Febrianto, Febrianto Fitriana Fitriana Frederik Palallo Gaffar, Rahmah Hadi, Umar Hambali, Amiruddin Hariyani Hariyani, Hariyani Hartoto Hartoto Hartoto Hasniar Hasniar, Hasniar Hasrul Adan, La Ode Muhammad Hery Widijanto Hidayat, Faisal Hijriyah, Ulfatul Husain Syam Isma, Isma Izmi Azhara Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamaluddin P, Jamaluddin P Jamaluddin P. Jayadi, Faisal K, Nuraini Kaseng, Ernawati Syahruddin Khaidir Rahman La Ode Ismail Ahmad, La Ode Ismail Lahming Lahming Lahming, Lahming M. Ichsan Ali Maedy, Herju Mahmuda, Dewi Mardiyansyah, Ferdi Maslina, Maslina Mewarni, Sella Miftahurrahmah Miftahurrahmah, Miftahurrahmah Muammar Muammar, Muammar Mubarak, Ahmad Zaky Muh. Syilfa Nooviar MUHAMMAD ALI Mukhlis, Andi Muhammad Akram Mukhtar, Andi Hijaz Naping, Hamka Nilwana, Andi Nirwana, Lina Novitasari, Ervi Nunik Lestari Nuraini Nuraini Nurdin, Rahmat Nurlita Pertiwi Nurmila, Nurmila Patang Patang Patang, Patang Qarimah, Inayah Ridhayanti Rafli, Muhammad Rahman, Khaidir Rahmat, Muliawan Ramli, Hartini Reski Praja Putra Risdawati, Risdawati Risnawati Risnawati Rito Goejantoro, Rito Rustan, Rustan S, Muliani Safitri, Ameliya SARASWATI SARASWATI Sella Mawarni Sinar, Purnama Sinring, Abdullah Sinta Nurul Oktaviana Kasim Siregar, Arnisah Suciani Latif Sukendi Sukendi Sukmawati Sukmawati Sultamin, Muhammad Sulhan Surya Prangga susanti, septria Syah, Maenuddin Bustanil Syahrul Syarif, Irwan Syarifuddin Syarifuddin TAHA, SYAMSUMARLIN Tanty, Ardianty Turangan, Hasni Utomo, Munzir Wulandari Wulandari Yanti, Nur Eva Yasa, Wayan Kamar yuli sari fatma yuli sari fatma Yunadya, Yunadya