F Agustin
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Journal : MEDIA PETERNAKAN - Journal of Animal Science and Technology

Inkorporasi Kromium oleh Fungi Ganoderma lucidum dengan Limbah Industri Kelapa Sawit Sebagai Substrat F Agustin; T Toharmat; D Evvyernie; D Taniwiryo; S. Tarigan; I Prihantono
Media Peternakan Vol. 33 No. 1 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.266 KB)

Abstract

The aim of this study was to determine the effective Cr (chromium) level for Ganoderma lucidum growth in solid state fermentation of oil palm by-product. Treatments were combination of Cr level (0, 500, 1000, 1500, 2000, 25000, and 3000 ppm) and fermentation time (0, 2, 4, 6, and 8 weeks).  The treatments were allocated in a factorial 7x5 of complete randomized design with four replications. Inoculant of G. lucidum was grown in potato dextrosa agar (PDA) medium for 10 days and then innoculated to substrate which have been autoclaved and mixed with CrCl3.6H2O. The moisture of substrate was maintained at 65%. Growth media of G. lucidum was diluted with aquades and the supernatant was analysed for its Cr content.  The result showed that the addition of Cr up to 3000 ppm into the medium stimulated the G. lucidum growth in all experimental condition. The Cr ions were incorporated into the media and G. lucidum cells during fermentation. Incorporation of chromium by G. lucidum was higher in oil palm by-product substrate with 3000 ppm Cr than the others. It is concluded that Cr can be incorporated into the G. lucidum cells during fermentation. The effective level of Cr for G. lucidum growth was 3000 ppm with efficiency of Cr incorporation 68.23% in 8 weeks fermentation and chromium in protein of fermentation product was 12.01%. Key words: chromium, Ganoderma lucidum, oil palm by-product
The Incorporation of Chromium in Rice Straw Fermented with Ganoderma lucidum F Agustin; T Toharmat; D Evvyernie; D Taniwiryono; S Tarigan
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.395 KB) | DOI: 10.5398/medpet.2013.36.1.64

Abstract

The research was designed to evaluate chromium incorporation by Ganoderma lucidum in rice straw substrate supplemented with chromium chloride. Treatments were combination of Cr level (0, 500, 1000, 1500, 2000, 25000, and 3000 ppm) and fermentation time (0, 2, 3, and 4 wk). The treatments were arranged in a factorial 7 x 4 and allocated in completely randomized design with three replications.  G. lucidum was grown in potato dextrose agar (PDA) medium for 10 days and was inoculated to the substrate which have been sterilized and mixed with CrCl3.6H2O. The moisture of substrate was maintained at 70%. Fiber and protein components of growth media of G. lucidum was determined and analyzed for their Cr content. The finding result showed that addition of Cr up to 3000 ppm into the substrate stimulated the G. lucidum growth.  Chromium was incorporated into the fiber and protein components of the growth substrate of G. lucidum during fermentation. Incorporation of Cr into the protein of substrate containing3000 ppm Cr  was highest when fermented for 4 wk.  Protein component of substrate contained 9.29% Cr while in NDF and ADF was 27.20% and 10.55% Cr, respectively. It is concluded that Cr was incorporated into the G. lucidum cells during fermentation.