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All Journal Biospecies Kolaboratif Bioeducation Journal Penelitian Pendidikan Biosfer: Jurnal Tadris Biologi Medical Journal of Indonesia BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan QUANTUM: Jurnal Inovasi Pendidikan Sains BIOMA : Jurnal Biologi Makassar Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Basicedu Jurnal Ilmu Kedokteran dan Kesehatan Biota Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Journal of Stem Cell Research and Tissue Engineering Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Ekologi, Masyarakat dan Sains FORUM PAEDAGOGIK Jurnal Biosense BiosciED: Journal of Biological Science and Education Halal Research Journal TROPICAL GENETICS Jurnal Vokasi Mekanika Al Jahiz: Journal of Biology Education Research Jurnal Basicedu Tropical Genetics Jurnal Serambi Biologi Jurnal Pendidikan IPA Indonesia
Yuni Ahda
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Journal : Jurnal Biogenerasi

 1 
DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE Hidayah, Nurul; Ahda, Yuni; Achyar, Afifatul; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5954

Abstract

EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.
DESAIN PRIMER GEN EDNRB EKSON 6 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG’S DISEASE Yuda Meisya, Divia; Ahda, Yuni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hirschsprung’s Disease (HSCR) is a congenital disorder characterized by the absence of ganglion cells in the intestinal wall, leading to impaired intestinal motility. One of the key genes involved in the pathogenesis of HSCR is the Endothelin Receptor Type B (EDNRB) gene, particularly mutations in exon 6. Detecting these mutations requires specific primers and well-optimized PCR conditions. This study aims to design specific primers for exon 6 of the EDNRB gene using in silico methods and to determine optimal PCR conditions through annealing temperature and primer concentration optimization. Primers were designed based on the EDNRB gene sequence (Accession No. NG_011630.3) using Geneious Prime and validated for specificity using Primer-BLAST. PCR optimization was conducted in vitro using various annealing temperatures 52.8–55.3°C and primer concentrations 0.5–1.1 µM on normal human DNA samples. PCR products were analyzed by 1.5% agarose gel electrophoresis. The primer design for exon 6 of the EDNRB gene yielded the following results: both forward and reverse primers were 20 bp in length, with melting temperatures (Tm) of 58.1°C and 56.1°C, and GC contents of 55% and 50%, respectively. In silico analysis confirmed primer specificity to chromosome 13. Optimal PCR conditions were achieved at an annealing temperature of 55.3°C and a primer concentration of 0.7 µM, yielding a single amplicon of 517 bp.
MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN KUMIS KUCING ( Orthosiphon aristatus) Ayesa, Putri; Fadhil Ramadhan, Bintang; Fitria, Davina; Mutia, Ravena; Ahda, Yuni; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.6781

Abstract

This study aims to evaluate the effect of cat whisker leaf extract (Orthosiphon aristatus) on motility and viability of male mice spermatozoa (Mus musculus L.). The research method used was experimental. This study was conducted to determine the effect of the treatment given to a matter under study. Consists of three treatments, namely control (without treatment), treatment dose 0.5 mg and treatment dose 0.8 mg Cat whisker leaf extract is given orally for 15 days. After treatment, spermatozoa were taken from the epididymis of male mice and tested for motility and viability using statistical analysis through the One-Way Anova test. The results showed that the decrease in motility and viability of spermatozoa depended on the dose given. The higher the dose of cat whisker extract, the motility and viability of spermatozoa decreased. In mice with a dose of 0.8 mg spermatozoan motility is silent and does not move and for viability it dies. The control treatment showed different motility but remained alive with good viability. Meanwhile, the treatment group showed a decrease in motility and viability, with control mice.
Co-Authors Abdul Rasak Abdul Razak Abdul Razak Achyar, Afifatul Afandi, Echa Azkia Afriandi, Muhammad Agusminarti, Agusminarti Ahmad Fauzan Ahmad Saleh Akbar, Imam Qodri Ambiyar, Ambiyar Ananda, Azwir Anggun, Septiasri Angreni, Siska Anisha S Anni Faridah Arfianti, Fina Aripin, Nurul Atifah, Yusni Ayesa, Putri Ayu, Duni Azalia, Salsa Novela Azeli, Saskia Putri Azwir Anhar Azzahra, Yolanda Belia Putri BR Tarigan, Siti Nadiah Zahra D. N. Putri, Dini Daniel Happy Putra Darmanella Dian Eka Wati Darussyamsu, Rahmawati Dezi Handayani Diana Lyrawati Diana Susanti Dualom, Siti Dwi Hilda Putri Efdillah, Syilvia Eriza, Deni Septia Evi Suryawati Fadhil Ramadhan, Bintang FAJRINA, SUCI Fatni Mufit Ferdyan, Rhavy Festiyed Festiyed Fidela, Wita Fitri Arsih Fitri, Anisa Fitri, Lel Fitria, Davina Fitria, Yanti Gunawan, Revi Gina Haryanto Haryanto Hastutin, Sri Yuni Hayu, Elvira Heffi Alberida Herisanti, Dini Ilahi, Threesia Anugrah Indra Hartanto Iqra, Kuntum Nurul IRDAWATI Irdes Hidayana Siregar Jörg Gromoll Junil Adri K. Sari, Jelly Keiko Kasy Billah Keiko Kasy Billah Khairunnisa Lasmana, Oria Linda Advinda Linda Wati Lufri Lufri Marcloryne Agnesia Masyitoh Masyitoh Maulina, Adinda Rizky Meisya, Divia Yuda Miftahul Jannah Monica, Della Trya Muhammad Ikbal Mulia, Nanta Muslima, Risa Ukhti Mutia Dwi Herakasih Mutia, Ravena Najib Rahman. G Neng Sholihat Nining Nuraida Nukman Moeloek Nurbaiti, Desy Nurpatri, Yeni NURUL HIDAYAH P. Ningky, Yiyin Pratama, Sandi Fransisco Purnomo Soeharso Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Ramadhan Sumarmin Ratih Komala Dewi Ratna Nila Sari Regina Rinti Mutiara Sari Rona Taula Sari, Rona Taula safitri, fira Sagurung, Mariance Dartiani Salsabil, Velina Samsudin, Syakirah Santosa, Tomi Apra Sari, Rinti Mutiara Sefina, Nadia Sepriyani, Eria Marina Septelia I. Wanandi Siagian, Gunaria Siregar, Titik Ermila Sri Suciati Ningsih Syafrilianto, Syafrilianto Syahrastani, Syahrastani Syamsurizal Syari, Elsy Melia T. Berlian Taufiqa, Zuhrah Valofi, Nagra Aulia Vivi Fitriani Wahyuni, Nur Anisa Wahyuni, Sisri Winda Ayu Fietri Yerimades, Yerimades Yerimadesi Yerimadesi Yerimadesi Yuda Meisya, Divia Yusi Febriani Zalni, Tita Yunia Zega, Dwi Junita Zhafira