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All Journal Bioma : Jurnal Biologi Indonesia Journal of Industial Engineering and Technology
Sri Amini
Pusat Riset Pengolahan Produk dan Sosial Ekonomi Kelautan dan Perikanan, Badan Riset Kelautan dan Perikanan, Departemen Kelautan dan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Industrial & Manufacturing Engineering

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Journal : Bioma : Jurnal Biologi Indonesia

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PERTUMBUHAN DAN AKTIVITAS ANTIOKSIDAN DARI MIKROALGA RAFAELINA, MONIKA; RUSTAM, YOSWITA; AMINI, SRI
Bioma Vol. 11 No. 1 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.647 KB) | DOI: 10.21009/Bioma11(1).2

Abstract

ABSTRACT Microalgaebiomass containschemicalcomponentssuch as vitamin, proteins, ω-3 and ω-6 polyunsaturated fatty acid and pigments. components with antioxidant activities can be found in only a few species of algae. The potential species of microalgae who have compounds act as antioxidant are Porphyridium cruentum and Chlorella sp. This study aimed to know the growth and antioxidant activity of P. cruentum and Chlorella sp. with the treatment of different cultures. Experimental method and completely randomized design was used as the research method. Antioxidant activity (Ic50 value) was measured by DPPH method (2,2-diphenyl-1-pikrilhidrazil). SPSS 16.0 is used to analyze the data with General Linier Model (GLM) Univariate. The results of GLM Univariate test for cell density showed that there was significant different for cell density in species factors (p= 0,000), but there was no significant different for cell density in culture factors (p= 0,375), and GLM Univariate test for antioxidant activity showed that no significant different for antioxidant activity in species factors (p= 0,522) but siginificant different for antioxidant activity in culture factors (p= 0,001). There was significant different for growth in P. cruentum and Chlorella sp. but no significant different in indoor and outdoor culture, as well as there was no significant different antioxidant activity in two species but significant different in indoor and outdoor culture. Keywords: growth, antioxidant activity, indoor cultivation, outdoor cultivation.
KULTIVASI DAN EKSTRAKSI MINYAK DARI MIKROALGA Botryococcus braunii DAN Nannochloropsis sp. musdalifah, Musdalifah; Rustam, Yoswita; Amini, Sri
Bioma Vol. 11 No. 2 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (697.457 KB) | DOI: 10.21009/Bioma11(2).1

Abstract

AbstractMicroalgae has high content of vegetable oil. The extraction of microalgae oil can be more efficient with the cell wall destruction, it was aimed to freeing oil that locked inside the cells and be soluble in organic solvent (n-hexane). The study aimed to determined the microalgae growth, the influence of different pH and different species of microalgae with extraction using microwave on oil production by Botryococcus braunii and Nannochloropsis sp. Experimental method with the randomized design was used in this study. The cultivation was performed until the stationery phase is reached so that the biomass can be harvested for oil extraction. The oil compound analysis is conducted using GC-FID. The data in this study has been tested by SPSS 16.0 with t-test and anova two ways. The statistic result showed that there is no differences in cell density of Botryococcus braunii and Nannochloropsis sp. (p= 0,163), likewise with there is no differences in amount of oil extract by Botryococcus braunii and Nannochloropsis sp. (p= 0,323). However, different results are pH treatment influencing oil extraction result (p= 0,006).Composition of fatty acid in Nannochloropsis sp. was dominated by saturated fatty acids, while the fatty acid composition of Botryococcus braunii was equal between saturated an unsaturated fatty acid. Keywords: Botryococcus braunii, cultivation, extraction, growth, microalgae, Nannochloropsis sp., oil, pH.
Co-Authors Ajeng Alifiana, Mia Meldra, Delia Musdalifah Musdalifah RAFAELINA, MONIKA RUSTAM, YOSWITA Sokhibi, Akh