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All Journal Biotika: Jurnal Ilmiah Biologi Bionatura Farmaka Indonesian Journal of Applied Sciences Jurnal Ilmu Dasar Jurnal Natur Indonesia Jurnal Radioisotop dan Radiofarmaka Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Global Medical and Health Communication Indonesian Journal of Science and Technology MPI (Media Pharmaceutica Indonesiana) INDONESIAN JOURNAL OF PHARMACY Pharmascience IDJP (Indonesian Journal of Pharmaceutics) Indonesian Journal of Chemistry PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Majalah Farmasetika Jurnal Ilmiah Farmako Bahari Indonesian Journal of Biological Pharmacy Jurnal Sains dan Teknologi Farmasi Indonesia Praeparandi : Jurnal Farmasi dan Sains Jurnal Sains dan Kesehatan
Anas Subarnas
Departemen Farmakologi Dan Farmasi Klinik, Fakultas Farmasi, Universitas Padjadjaran, Sumedang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health

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Journal : Indonesian Journal of Pharmaceutical Science and Technology

 1 
Antioxidant Activity of Combination Ethanol Extract of Turmeric Rhizome (Curcuma Domestica Val) and Ethanol Extract of Trengguli Bark (Cassia Fistula L) with DPPH Method Triyono, Triyono; Yohana Chaerunisaa, Anis; Subarnas, Anas
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.508 KB) | DOI: 10.24198/ijpst.v5i3.14764

Abstract

Turmeric rhizome (Curcuma domestica Val) and Trengguli bark (Cassia fistula L) contain antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl (DPPH) free radical inhibition method. This research was conducted to determine DPPH free radical inhibition by ethanol extract of turmeric rhizome, the ethanol extract of trengguli bark, and a combination of turmeric rhizome extract - trengguli bark extract (1:1,5) with ascorbic acid as a comparison. Identification of secondary metabolite classes is performed by phytochemical screening. Antioxidant activity was performed by inhibition of free radical color of DPPH using UV-Vis spectrophotometry. The study showed IC50 value of ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of trengguli bark has the best antioxidant activity with IC50 value 10,98 μg/mL compare to combination ethanol extract of turmeric rhizome - trengguli bark (1 :1,5) and ethanol extract of turmeric rhizome with IC50 value is 13,70 μg/mL and 41,95 μg/mL, respectively.Keywords: Antioxidant, Curcuma domestica, Cassia fistula, DPPH Method
AKTIVITAS ANTIOKSIDAN EKSTRAK dan FRAKSI KULIT BATANG TRENGGULI (Cassia fistula L) DENGAN METODE DPPH Najihudin, Aji; Chaerunisaa, Anis; Subarnas, Anas
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 2 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.972 KB) | DOI: 10.15416/ijpst.v4i2.12354

Abstract

Kulit batang trengguli (Cassia fistula L) mengandung senyawa yang bersifat antioksidan yang dapat ditentukan dengan metode peredaman radikal bebas 1,1-Difenil-2- Pikrilhidrazil (DPPH). Penelitian ini dilakukan untuk mengetahui adanya peredaman radikal bebas DPPH oleh ekstrak etanol kulit batang trengguli (EEKBT), fraksi Etil asetat (FEAKBT), fraksi n-Heksan (FnHKBT), fraksi air (FAKBT) dan vitamin C sebagai pembanding. Identifikasi golongan senyawa dilakukan dengan penapisan fitokimia. Fraksinasi dilakukan dengan metode ekstraksi cair-cair dengan tingkat kepolaran yang berbeda. Aktivitas antioksidan dilakukan dengan peredaman warna radikal bebas DPPH menggunakan spektrofotometri UVVis. Penelitian menunjukkan bahwa Fraksi etil asetat memiliki aktivitas antioksidan paling baik dengan nilai IC50 3,980 μg/ml dibandingkan vitamin C 4,716 μg/ml. Sedangkan nilai IC50 ekstrak etanaol, fraksi n-heksan, fraksi air adalah 10,613 μg/ml, 38,904 μg/ml dan 7,636 μg/ml terhadap peredaman warna DPPH.Kata kunci: Antioksidan, Cassia fistula, Fraksinasi, metode DPPH
AKTIVITAS ANTIOKSIDAN EKSTRAK dan FRAKSI KULIT BATANG TRENGGULI (Cassia fistula L) DENGAN METODE DPPH Aji Najihudin; Anis Chaerunisaa; Anas Subarnas
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 2 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.972 KB) | DOI: 10.15416/ijpst.v4i2.12354

Abstract

Kulit batang trengguli (Cassia fistula L) mengandung senyawa yang bersifat antioksidan yang dapat ditentukan dengan metode peredaman radikal bebas 1,1-Difenil-2- Pikrilhidrazil (DPPH). Penelitian ini dilakukan untuk mengetahui adanya peredaman radikal bebas DPPH oleh ekstrak etanol kulit batang trengguli (EEKBT), fraksi Etil asetat (FEAKBT), fraksi n-Heksan (FnHKBT), fraksi air (FAKBT) dan vitamin C sebagai pembanding. Identifikasi golongan senyawa dilakukan dengan penapisan fitokimia. Fraksinasi dilakukan dengan metode ekstraksi cair-cair dengan tingkat kepolaran yang berbeda. Aktivitas antioksidan dilakukan dengan peredaman warna radikal bebas DPPH menggunakan spektrofotometri UVVis. Penelitian menunjukkan bahwa Fraksi etil asetat memiliki aktivitas antioksidan paling baik dengan nilai IC50 3,980 μg/ml dibandingkan vitamin C 4,716 μg/ml. Sedangkan nilai IC50 ekstrak etanaol, fraksi n-heksan, fraksi air adalah 10,613 μg/ml, 38,904 μg/ml dan 7,636 μg/ml terhadap peredaman warna DPPH.Kata kunci: Antioksidan, Cassia fistula, Fraksinasi, metode DPPH
Antioxidant Activity of Combination Ethanol Extract of Turmeric Rhizome (Curcuma Domestica Val) and Ethanol Extract of Trengguli Bark (Cassia Fistula L) with DPPH Method Triyono Triyono; Anis Yohana Chaerunisaa; Anas Subarnas
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.508 KB) | DOI: 10.24198/ijpst.v5i2.14764

Abstract

Turmeric rhizome (Curcuma domestica Val) and Trengguli bark (Cassia fistula L) contain antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl (DPPH) free radical inhibition method. This research was conducted to determine DPPH free radical inhibition by ethanol extract of turmeric rhizome, the ethanol extract of trengguli bark, and a combination of turmeric rhizome extract - trengguli bark extract (1:1,5) with ascorbic acid as a comparison. Identification of secondary metabolite classes is performed by phytochemical screening. Antioxidant activity was performed by inhibition of free radical color of DPPH using UV-Vis spectrophotometry. The study showed IC50 value of ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of trengguli bark has the best antioxidant activity with IC50 value 10,98 μg/mL compare to combination ethanol extract of turmeric rhizome - trengguli bark (1 :1,5) and ethanol extract of turmeric rhizome with IC50 value is 13,70 μg/mL and 41,95 μg/mL, respectively.Keywords: Antioxidant, Curcuma domestica, Cassia fistula, DPPH Method
Determination and Purification of Ricin Protein from Ricinus communis L. Seeds using CLC (Column Liquid Chramotagoraphy) and FPLC (Fast Protein Liquid Chramatography) Irma Erika Herawati; Ronny Lesmana; Jutti Levita; Anas Subarnas
Indonesian Journal of Pharmaceutical Science and Technology Vol 8, No 3 (2021)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v8i3.35454

Abstract

Proteins found in all biological systems, from prokaryotes to eukaryotes. Ricin, one of the most toxic substances known isolated from Ricinus communis L. seeds, is a heterodimeric two-domain polypeptide protein that includes chain A (30 kDa) and chain B (35 kDa) linked by a disulfide bond. Ricin binds to cells by the B chain and is then internalized. Ricin has been reported as a potential chemical for cancer treatment. The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research. However, so far, the quantification of ricin protein is not much reported. In this study, the quantification ricin protein extracted from R. communis L. seeds originated from Nganjuk, East Java, Indonesia. The techniques used column liquid chromatography (CLC) and fast protein liquid chromatography (FPLC), followed by quantification protein content used Bradford method. Results showed that all techniques positively confirm the presence of ricin protein. Ricin protein content were 0.171±0.021 mg/mL and 0.382±0.023 mg/mL using CLC and FPLC respectively. This study might contribute to understanding the biological and chemical properties of the ricin protein of R. communis L. seeds. Ricin protein content measured with FPLC were higher than CLC. Keywords: Ricin, Ricinus communis,CLC, FPLC.
Co-Authors Abdul Mutalib Abdul Mutalib Adang Hardi Gunawan Adryan Fristiohady Afiat Berbudi Agung W.M. Yodha Aji Najihudin ALIF VIRISY BERLIAN AMELIA SUCI PRAFITRI YANI Ami Tjitraresmi Andini Sundowo Ani Haerani ANI HAERANI Anis Chaerunisaa Anis Yohana Chaerunisa ANIS YOHANA CHAERUNISA Anis Yohana Chaerunisaa Anis Yohana Chaerunisaa Anton Apriyantono ASENG RAMLAN Asep Nurrahman Yulianto ASMAN SADINO, ASMAN Aulia Annisa Putri Heri Belinda, Rahma BRIGITTA TIARA PUTRI PRIYANTONO California, Salma H. Chaerunisaa, Anis Cucu Hadiansyah Dana Nasrullah Dani Sujana Dea A. A. Kurniasih DIAH SITI FATIMAH DIANTINI, AJENG - Dicky Santosa Dida Akhmad Gurnida Dika Destiani Dingse Pandiangan - ELI HALIMAH Eli Halimah, Eli Ellin Febrina Elvan Kamal Endang Sutedja Eri Amalia Euis Julaeha Fauzia Azzahra Fazrina Pratiwi Fransiska Silalahi Henni Djuhaeni Henni Djuhaeni Herri S. Sastramihardja Hesti Renggana Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi HIRALIUS BIMA ARDIKA PUTRA I. Sahidin IIN SUPARTINAH NOER Indradi, Raden Bayu Irma Erika Herawati Iyan Sopyan Joharudin, Ade Juliani Ronike Turang Jutti Levita Karyono - Keri Lestari Khoirunnisa, Afina Khoziah Ahmad, Khoziah Kunkun J. Gurmaya Kurniasih, Dea A. A. Lika Ginanti Febriana Lutfi Sulaiman M. S. Soedjanaatmadja Magdarita Riwu Mardhiani, Yanni Dhiani Marline Abdassah Bratadiredja Martalena Ramli Maulana Yusuf Alkandahri Mega Juliana Moelyono Moektiwardoyo, Moelyono Mohammad Ghozali Muchtaridi Muchtaridi Mutiara, Intan Najihudin, Aji Nasrudin Nasrudin Nela - Simanjuntak NONI CAHYANA Noor Rain Abdullah Noor Rain Abdullah Nor Latifah Norisca Aliza Putriana NUR ALFI KUSUMAH DEWI PARAS WATI Pramesti, Ardhia Kirana Prayudi Ahmad Puja Yanti Raden Maya Febrianti Rano K. Sinuraya Ratu Ralna Ismuha Renggana, Hesti Resmi Mustarichie Riezki Amalia Risrina Nur Ekawati Rizkita Rahmi Esyanti - Ronny Lesmana Salma H. California Shinta Marpaung Sinuraya, Rano K. Siti Saidah Muthmainah Slamet Budijanto Sofa Fajriah Sri Adi Sumiwi Sri Hartini Sriwidodo Sriwidodo Supriyatna ., Supriyatna Supriyatna Supriyatna Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Surachman, Emma Suseno Amien Suwandi, Deden Winda Syafrudin Purnama Sidiq, Syafrudin Purnama Taofik Rusdiana Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tina Rostinawati Triyono Triyono Triyono Triyono Unang Supratman Utami, Putri Antiyani W. Wahyuni Wenny Tilaar - Yohana Chaerunisaa, Anis Yoppi Iskandar Yulianto, Asep Nurrahman Yuni Elsa Hadisaputri