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Journal : Journal of Aquaculture Management and Technology

PENGARUH PEMBERIAN REKOMBINAN HORMON PERTUMBUHAN (rGH) MELALUI METODE PERENDAMAN DENGAN LAMA WAKTU YANG BERBEDA TERHADAP PERTUMBUHAN DAN KELULUSHIDUPAN LARVA BAWAL AIR TAWAR (Colossoma macropomum Cuv) Atmojo, Aditya; Basuki, Fajar; Nugroho, Ristiawan Agung
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Permintaan pasar bawal air tawar yang tinggi harus didukung dengan usaha budidaya yang berkelanjutan dan siklus produksi yang cepat. Rekombinan hormon pertumbuhan (rGH) merupakan inovasi teknologi dibidang perikanan yang memiliki potensi sebagai pakan suplemen yang diharapkan dapat memberikan percepatan pertumbuhan pada ikan budidaya. Tujuan dari penelitian ini adalah untuk mengetahui lama waktu perendaman rGH yang terbaik terhadap pertumbuhan dan kelulushidupan larva bawal air tawar (C. macropomum). Penelitian ini dilakukan dengan metode eksperimen Rancangan Acak Lengkap (RAL) dengan 4 perlakuan 3 pengulangan. Ikan uji yang digunakan adalah bawal air tawar dengan bobot individu rata – rata 2,59±0,32 g/ekor dan panjang 4,5 – 5,5 cm. Ikan uji dipelihara selama 45 hari dengan padat tebar 1 ekor/l. Dosis rGH yang digunakan adalah 2,5 mg/l. Perlakuan A tanpa perendaman, B perendaman 30 menit, C perendaman 60 menit, D perendaman 90 menit. Data yang diamati meliputi tingkat konsumsi pakan (TKP), rasio konversi pakan (FCR), laju pertumbuhan spesifik (SGR), pertumbuhan panjang mutlak (L), kelulushidupan (SR) dan kualitas air. Hasil pengamatan total konsumsi pakan perlakuan A sebesar 193,72±2,96g, perlakuan B sebesar 193,06±4,55g, perlakuan C sebesar 193,81±10,32g, perlakuan D sebesar 192,01±3,85g. Nilai rasio konversi pakan perlakuan A sebesar 1,07±0,09, perlakuan B sebesar 1,11±0,05, perlakuan C sebesar 1,10±0,1, perlakuan D sebesar 0,99±0,01. Laju pertumbuhan spesifik didapatkan hasil A sebesar 3,44±0,03%/hari, perlakuan B sebesar 3,36±0,07%/hari, perlakuan C sebesar 3,45±0,06%/hari, perlakuan D sebesar  3,58±0,03%/hari. Pertumbuhan panjang mutlak diperoleh hasil perlakuan A sebesar 3,51±0,26cm, perlakuan B sebesar 3,45±0,21cm, perlakuan C sebesar 3,46±0,10cm, perlakuan D sebesar 3,95±0,06cm. Nilai kelulushidupan diperoleh perlakuan A sebesar 90,00±8,66%, perlakuan B sebesar 88,33±2,89%, perlakuan C sebesar 90,00±8,66%, perlakuan D sebesar 93,33±2,89%. Kualitas air pada media pemeliharaan terdapat pada kisaran yang layak untuk ikan uji. Pemberian rGH melalui metode perendaman dengan lama waktu 90 menit dapat meningkatkan laju pertumbuhan spesifik dan pertumbuhan panjang mutlak bawal air tawar. Market demand on red-bellied pacu should be responded by sustainable and fast production cycle. Recombinant Growth Hormone was a technology innovation in fisheries sector which has potential ability that hopefully could give a growth acceleration on aquaculture organism. The aim of this research was to found the best duration of Recombinant Growth Hormone effects through immersion method on growth and survival rate of red-bellied pacu (C. macropomum) fry. This research was used an experimental method of completely randomized design for 4 treatments and 3 replicates. The trial fish was red-bellied pacu (C. macropomum) with 2,59±0,32 g/fish body weight average and 4,5 – 5,5 cm height. The fish was cultured for 45 days with 1 fish/l stocking density. The dose of rGH was 2,5mg/l. The treatments of this research were: A that was for trial with no rGH immersion, B with 30 minutes of rGH immersion, C with 60 minutes of rGH immersion, and D with 90 minutes rGH immersion. The measured data included the feed consumption, feed convertion ratio (FCR), specific growth rate (SGR), absolute length growth (L), survival rate (SR), and water quality. The result showed total feed consumption treatment A 193,72±2,96g, treatment B 193,06±4,55g, treatment C 193,81±10,32g, treatment D 192,01±3,85g. Feed convertion ratio treatment A 1,07±0,09, treatment B 1,11±0,05, treatment C 1,10±0,1, treatment D 0,99±0,01. Specific growth rate treatment A 3,44±0,03%/day, treatment B 3,36±0,07%/day, treatment C 3,45±0,06%/day, treatment D 3,58±0,03%/day. Absolute length growth treatment A 3,51±0,26cm, treatment B 3,45±0,21cm, treatment C 3,46±0,10cm, treatment D 3,95±0,06cm. Survival rate treatment A 90,00±8,66%, treatment B 88,33±2,89% SR, treatment C 90,00±8,66%, treatment D 93,33±2,89%. Water quality parameters during rearing period were suitable for the trial fish. Giving rGH through immersion method with 90 minutes duration could increased the specific growth rate and absolute length growth of red-bellied pacu.
PENGARUH LAMA PERENDAMAN HORMON TIROKSIN TERHADAP DAYA TETAS TELUR, PERTUMBUHAN, DAN KELANGSUNGAN HIDUP LARVA IKAN MAS KOKI (Carassius auratus) Manurung, Septina; Basuki, Fajar; Desrina, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan Mas Koki (Carassius auratus) merupakan salah satu jenis ikan hias yang sangat digemari masyarakat serta memiliki nilai ekonomis yang sangat tinggi. Namun derajat penetasan dan pertumbuhan ikan mas koki sangat rendah. Salah satu upaya yang dapat dilakukan untuk mempercepat daya tetas, pertumbuhan dan kelangsungan hidup larva ikan mas koki yaitu melalui ransangan hormonal seperti hormon tiroksin. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian hormone tiroksin terhadap HR, SR larva ikan mas koki (C. auratus) dan untuk mengetahui pengaruh lama waktu perendaman hormon tiroksin yang terbaik untuk meningkatkan daya tetas, pertumbuhan serta kelangsungan hidup larva ikan mas koki (C. auratus). Penelitian ini menggunakan metode eksperimental dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini yaitu Perlakuan A (control), B (perendaman hormon tiroksin 8 jam dengan dosis 0,10 mg/l), C (perendaman hormon tiroksin 16 jam dengan dosis 0,10 mg/l), dan D (perendaman hormon tiroksin 24 jam dengan dosis 0,10 mg/l). Data yang diamati meliputi daya tetas telur (HR), survival rate (SR), laju pertumbuhan spesifik, dan kualitas air. Hasil penelitian menunujukkan bahwa daya tetas telur ikan mas koki pasca perendaman hormon tiroksin berpengaruh nyata (P≤0,01) dengan hasil terbaik terdapat pada perlakuan D 77.67%±1.15, sedangkan tingkat kelulushidupan serta laju pertumbuhan spesifik juga menunjukkan tidak berpengaruh nyata (P≥0,01). Sedangkan kualitas air selama pemeliharaan masih dalam kisaran layak untuk budidaya ikan mas koki. Goldfish (C. auratus) is one type of ornamental fish that is very popular community and has a very high economic value. But the degree of hatching and growth of the goldfish is very low. One effort that can be done to accelerate the hatchability, growth and survival of goldfish larvae is through hormonal stimulation such as thyroxine hormone. This study aims to determine the effect of thyroxine hormone on HR, SR chef larvae (C. auratus) and to determine the effect of longest time of thyroxine hormone immersion to increase hatchability, growth and survival of goldfish larvae (C. auratus). This research used experimental method with 4 treatments and 3 replications. The treatments in this study were Treatment A (control), B (8 hours of thyroid hormone immersion with a dose of 0.10 mg / l), C (16 hours of thyroxine hormone with a dose of 0.10 mg / l), and D (hormone immersion 24 hours of thyroxine at a dose of 0.10 mg / l). Observed data included egg hatchability (HR), survival rate (SR), specific growth rate, and water quality. The results showed that the hatchability of goldfish eggs after soaking the hormone thyroxine significantly (P≤0,01) with the best results was found in treatment D 77.67% ± 1.15, whereas the survival rate and the specific growth rate also showed no significant effect (P≥ 0.01). While the quality of water during maintenance is still within a reasonable range for the cultivation of the goldfish.   Goldfish (C. auratus) is one type of ornamental fish that is very popular community and has a very high economic value. But the degree of hatching and growth of the goldfish is very low. One effort that can be done to accelerate the hatchability, growth and survival of goldfish larvae is through hormonal stimulation such as thyroxine hormone. This study aims to determine the effect of thyroxine hormone on HR, SR chef larvae (C. auratus) and to determine the effect of longest time of thyroxine hormone immersion to increase hatchability, growth and survival of goldfish larvae (C. auratus). This research used experimental method with 4 treatments and 3 replications. The treatments in this study were Treatment A (control), B (8 hours of thyroid hormone immersion with a dose of 0.10 mg / l), C (16 hours of thyroxine hormone with a dose of 0.10 mg / l), and D (hormone immersion 24 hours of thyroxine at a dose of 0.10 mg / l). Observed data included egg hatchability (HR), survival rate (SR), specific growth rate, and water quality. The results showed that the hatchability of goldfish eggs after soaking the hormone thyroxine significantly (P≤0,01) with the best results was found in treatment D 77.67% ± 1.15, whereas the survival rate and the specific growth rate also showed no significant effect (P≥ 0.01). While the quality of water during maintenance is still within a reasonable range for the cultivation of the goldfish.
PENGARUH PEMBERIAN REKOMBINAN HORMON PERTUMBUHAN (rGH) MELALUI METODE PERENDAMAN DENGAN LAMA WAKTU YANG BERBEDA TERHADAP PERTUMBUHAN DAN KELULUSHIDUPAN IKAN LELE VARIETAS SANGKURIANG Triwinarso, Wisnu Hadi; Basuki, Fajar; Yuniarti, Tristiana
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh pertumbuhan benih ikan lele sangkuriang yang diberi rekombinan hormon pertumbuhan dosis 2mg/L dengan metode perendaman dengan lama waktu yang berbeda dan mengkaji waktu yang optimal benih ikan lele sangkuriang yang diberi rekombinan hormon pertumbuhan terhadap pertumbuhan dan kelangsungan hidup benih ikan lele sangkuriang. Penelitian ini dilaksanakan di Satker PBIAT Ngrajek, Magelang, pada bulan Agustus-November 2013. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan perendaman dengan larutan rekombinan hormon pertumbuhan 2mg/L perlakuan A selama 0 menit, perlakuan B 30 menit, perlakuan C 60 menit, dan perlakuan D 90 menit. rGH yang digunakan berasal dari ikan kerapu kertang (ElrGH). Hasil pengamatan pertumbuhan bobot spesifik perlakuan A sebesar 5,642±0,025 %/hari, perlakuan B sebesar 6,510±0,055 %/hari, perlakuan C sebesar 6,358±0,108 %/hari, perlakuan D sebesar 6,240±0,179 %/hari. Pengukuran panjang mutlak didapat hasil perlakuan A mendapatkan hasil 4,18±0,07 cm, pelakuan B 5,35 ± 0,03 cm, perlakuan C 5,30 ± 0,09 cm, dan perlakuan D 5,25 ± 0,23 cm. Nilai konversi pakan pada perlakuan A 0,751±0,008, perlakuan B 0,457±0,022, perlakuan C 0,514±0,010, perlakuan D 0,543±0,008. SR yang didapat selama pemeliharaan pada perlakuan A 82,67±1,53%, Perlakuan B 79,00±3,00% , perlakuan C 75,33±2,52%, perlakuan D 73,00±1,00%. Pemberian rekombinan hormon pertumbuhan melalui metode perendaman pada ikan lele sangkuriang dengan lama waktu 30 menit dapat meningkatkan pertumbuhan bobot spesifik, pertumbuhan panjang mutlak, dan kelulushidupan, serta menurunkan rasio konversi pakan. Pemberian rekombinan hormon pertumbuhan dapat meningkatkan SGR sebesar 15,90%, panjang mutlak 28%, kelulushidupan 13,25%, dan menurunkan FCR 64,33% The purpose of this study was to assess the effect of seed growth sangkuriang catfish fed with recombinant growth hormone dose 2mg / L through immersion method with different time and assess the optimal time sangkuriang seed catfish fed recombinant growth hormone on growth and seed survival rate catfish sangkuriang. This study was conducted in Unit Freshwater Fish Hatchery Center (SATKER PBIAT) Ngrajek, Magelang, August-November 2013. Research using a completely randomized design (CRD) with 4 treatments and 3 replications. Immersion treatment with recombinant growth hormone solution of 2 mg / L treatment A for 0 min, 30 min treatment B, treatment of C 60 mins, and treatment D 90 minutes. The rGH used from giant grouper fish (rElGH). The observation of SGR treatment of A 5,642±0,025 %/day, treatment of B 6,510±0,055 %/day, treatment of C 6,358±0,108 %/day, treatment of D 6,240±0,179 %/day. The absolute length measurements that showed growth optimum results at A 4,18±0,07 cm, treatment of B 5,35 ± 0,03 cm, treatment of C 5,30 ± 0,09 cm, and treatment of D 5,25 ± 0,23 cm. Feed conversion value in treatment A 0,751±0,008, treatment B 0,457±0,022, treatment C 0,514±0,010, treatment D 0,543±0,008. SR obtained during the maintenance treatment A 82,67±1,53%, treatment B 79,00±3,00% , treatment C 75,33±2,52%, treatment D 73,00±1,00%. Administration of recombinant growth hormone through immersion method at catfish sangkuriang long 30 minutes can increase the growth of a specific weight, length of absolute growth, and survival, as well as lower feed conversion ratio. Administration of recombinant growth hormone can increase by 15.90% SGR, the absolute length of 28%, the survival of 13.25%, and 64.33% lower FCR