Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Nurita Toruan-Mathius
Department of Biotechnology, Plant Production and Biotechnology Division, PT.SMART Tbk Jl. Raya Cijayanti Kampung Pasirmaung-Sentul West Java 16810, Indonesia
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Identification of Gene Related to Hard Bunch Phenotype in Oil Palm (Elaeis guineensis Jacq.) Roberdi ,; Sobir ,; Sudirman Yahya; Nurita Toruan-Mathius; Tony Liwang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.814 KB) | DOI: 10.24831/jai.v43i2.10421

Abstract

ABSTRACTMolecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon
Co-Authors Roberdi , Sobir Sobir Sudirman Yahya Tony Liwang