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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Current Biochemistry Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Greensphere: Journal of Environmental Chemistry Prosiding Seminar Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Anto Budiharjo
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Berkala Bioteknologi

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Ekstraksi Dan Uji Stabilitas Zat Warna Daun Jambu Biji (Psidium guajava L.) Pardede, Lasria; Kusdiyantini, Endang; Budiharjo, Anto
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Guava leaves is known as a traditional medicine to treat various diseases, such as diarrhea, dengue fever, etc. Along with the development of technology, guava leafs is now used as a color contributor on textiles. This study aims to extract on the solvent distilled water and ethanol and to test the stability of guava leaf color pigment against the influence of storage temperature, time span of the addition of an oxidant and pH. Leaves extracted by soaked for 24 hours in distilled water solvent that has been preheated to 30°C, 50ºC, 70º C and 90ºC and ethanol with a concentration of 20%, 40%, 60%, 80% and 96%. Absorbance measurements of guava leaf extract is using spectrophotometer at a wavelength of 525 nm. The results showed that guava leaf extract has the optimum absorbance value on distilled water solvent at 90ºC and 20%ethanol. Stability test is done by storage temperature effect, oxidizing agents adding, and pH treatment. Stability test of guava leaf extract showed that extracted guava leaf color pigment is stable on 9°C storage temperature
Isolasi dan Identifikasi Molekuler Bakteri Pelarut Fosfat dari Gua Gamelan di Kawasan karst Kiskendo Kendal, Jawa Tengah Mahmudah, Hawari Rosdiana; Suprihadi, Agung; Budiharjo, Anto
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Phosphate solubilizing bacteria is a group of bacteria that dissolves unavailable phosphate into the form that can be absorbed by plants. This research aimed at isolation and moleculer identification of phosphate solubilizing bacteria based on 16s rRNA from bat cave guano and potential microorganisms which were able to dissolved phosphate. Several stages of this research are, isolation PSB in Pikovskayaand NBRIP agar, Gram staining, and molecularidentification. The isolation result obtained two isolates phosphate solubilizing bacteria, which are G4-1 and G5. Isolate G5 is the highest on solubilizing phosphate with a diameter of clear zone 22.01 mm, and Isolate G4-1 exhibited diameter of clear zone 19.8 mm. Isolates G4-1 and G5 are the gram-negative bacteria. DNA amplification of these bacteria employing universal primers 27 F (5’-AGAGTTTGATCMTGGCTCAG-3’) dan Primer 1492 R (5’-GGTTACCTTGTTACGACTT-3’) generated the 1520 bp and 1235 bp PCR product. The result of the analysis of 16s rRNA gene sequence showed that isolate G4-1 has 80% similarity with Acinetobacteriwofii strain-JCM partial sequence, and isolate G5 has 92% similarity with Serratia marcesens strain-NBRC.
Analisis SNP (Single Nucleotide Polymorphism) Promotor GEN IL-10 pada Penderita Filariasis Maria, Atina; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti
Berkala Bioteknologi Vol. 4, No. 2, November 2021
Publisher : Berkala Bioteknologi

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Filariasis limfatik merupakan suatu penyakit yang disebabkan oleh nematoda dari jenis Wuchereria bancrofti dan Brugia malayi dewasa yang menghuni jaringan limfatik. Filariasis limfatik ini disebarkan oleh empat genus nyamuk, yaitu, Anopheles, Culex, Aedes dan Mansonia. Respon imun terhadap parasit filaria terdiri dari Thelper2 (Th2) dan melibatkan produksi cytokin –IL-4, IL-5, IL-9, IL-10, dan IL-13, isotype antibody—IgG1, IgG4 and IgE, dan meningkatnya populasi eosinophils dan makrofag yang aktif. Polimorfisme pada daerah promotor gen IL-10 diidentifikasi berhubungan dengan tinggi rendahnya produksi IL-10 pada sel darah perifer pada stimulasi in vitro dengan lipopolysacharide (LPS). Sampel diambil dari penderita filariasis, kemudian diekstraksi dan diamplifikasi pada posisi -1082, -819, -592. Hasil amplifikasi kemudian di restriksi dan di sekuensing. Hasil penelitian dari sampel penderita filariasis yang diteliti menunjukan memiliki haplotip ACA pada analisis RFLP. Analisis hasil sekuensing menggunakan dua aplikasi, yaitu DNA baser dan Bioedit. Analisis menggunakan DNA baser dan Bioedit serta dibandingkan dengan tag SNP didapatkan satu SNP. Perbandingan antara sekuens DNA yang didapatkan dengan tag SNP mandapatkan haplotip CC.
Co-Authors AB SUSANTO Abhinaya, Danendra Daryl Ade Fajrian Adila Nawan Hasrimi Agung Suprihadi Agung Suprihadi Ahmad Thontowi Anggoro, Naufal Sebastian Aninditia Sabdaningsih Ariani, Nur Frida Arina Tri Lunggani Asgasatya, Syahid Fattah Barri Pratama Budi Raharjo Bunga Fajriani CATUR RIANI Chatarina Umbul Wahyuni Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Deverina Mergypta Dewantoro, Giwang Dian Wahyu Kemalaputri Dudi Hardianto, Dudi Dyah Ayu Riani Dyah Wulandari Edwin Setiawan Eko Purnomo Elfira, Yolanda Elke Gildantia Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Fahmi, Maya Fitriana Ilul Farikha, Siti Lutfiatul Fauzaan, Muhammad Faishal Fauziah Citra Rahmawati Fitri Arum Sasi, Fitri Arum Fitria, Lutfiah Rahmadini Florentina Kusmiyati HARTAJANIE, LAKSMI Hasrimi, Adila Nawan Heni Wijayanti, Heni Hermanto, Michael Einstein Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Husna, Faza Laili IS HELIANTI Khoirul Huda Kristina Kristina Kurniawati, Mufida Budi Kusumaningrum, Hermin Laila Nur Faizah Lasria Pardede Lindayani, Lindayani Luthfy AN M Masfuroh Maerani Sumarno Mahmudah, Hawari Rosdiana Maria, Atina Maulana, Anand Reyna Mawarni, Risa Arum Maya Fitriana Ilul Fahmi MG Isworo Rukmi Mia Tri Wardani Monalita, Ramadhebi Muhammad Adib Mubarok Muhammad Mulyadi, Muhammad Muhammad Zainuri Norma Sainstika Pangestu Novita Rahmawati, Novita Nur Endah Wahyuningsih Nurhayati Nurhayati Nurlita Abdulgani Octavia Dewi Christiningrum Olivia Nisa Mumtianah Pardede, Lasria Prayogo, Fitra Adi Rahayu, Annisa Rizky Rahmah Putri Sunarno Ratna Cempaka Lingga Rejeki Siti Ferniah Risky Panji Nugroho Risma Wiharyanti Safirah, Dearesty Saniha Adini, Saniha Sayono Setyo Nugroho Shabrina, Jauhara Siti Nur Jannah Siti Nur Jannah Siu S.S Langden Sonny Abdi Setiyawan, Sonny Abdi Soowanayan, Chumporn Sri Pujiyanto Suetrong, Satinee Sumarno, Maerani Suprihadi Susiana Purwantisari Teuku Tajuddin Thu'ti Alawiyyah Tony Hadibarata, Tony w wijanarka Wahyu Dewi U. Haryanti Wien Kusharyoto Wijanarka Wijanarka Yason Lukman Sudjito, Yason Lukman Yohanes Chandrawijaya Yopi Yopi Yuliandini Pangestika