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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Agromet Jurnal Ilmu dan Teknologi Kelautan Tropis Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Widyariset KOVALEN: Jurnal Riset Kimia Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Kelola: Journal of Islamic Education Management Indonesian Aquaculture Journal Jurnal Riset Akuakultur Journal of Educational Research and Evaluation BJRA (Bongaya Journal of Research in Accounting) JSMA (Jurnal Sains Manajemen dan Akuntansi) Widyariset GANDRUNG: Jurnal Pengabdian Kepada Masyarakat Siyasatuna Jurnal Ilmiyah Mahasiswa Siyasah Syariyyah Jurnal Aktiva : Riset Akutansi dan Keuangan Qadauna: Jurnal Ilmiah Mahasiswa Hukum Keluarga Islam Journal of Applied Civil Engineering and Infrastructure Technology (JACEIT) Jumper : Jurnal Mahasiswa Pendidikan Olahraga Jurnal Media Akuakultur Indonesia Shautuna: Jurnal Ilmiah Mahasiswa Perbandingan Mazhab Arus Jurnal Sosial dan Humaniora Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat SinarFe7 Private Law Journal of Applied Sciences in Accounting, Finance, and Tax Jurnal Kedokteran Hewan Jurnal Pengabdian Masyarakat Olahraga dan Kesehatan Vokatek : Jurnal Pengabdian Masyarakat PEDAMATH: Journal on Pedagogical Mathematics Al Fuadiy: Jurnal Hukum Keluarga Islam Economos : Jurnal Ekonomi dan Bisnis Journal Sultra Research of Law International Journal of Educational and Life Sciences (IJELS) Jurnal Media Akademik (JMA) ENLEKTURER: Journal of Islamic Studies HISABUNA: Jurnal Ilmu Falak Journal of Fisheries & Marine Inovasi Teknologi Masyarakat Journal of Mathematics, Computation and Statistics (JMATHCOS) International Journal of Education, Vocational and Social Science Hikamatzu Journal of Multidisciplinary Research Jurnal Bioteknologi & Biosains Indonesia (JBBI) Bersama: Jurnal Pengabdian Kepada Masyarakat Issues in Mathematics Educations (IMED) Lumbung Ngabdi: Jurnal Pengabdian Masyarakat At-Tadris: Journal of Islamic Education J-Sil (Jurnal Teknik Sipil dan Lingkungan)
Alimuddin
Departemen Budidaya Perairan, Fakultas Perikanan Dan Kelautan, Institut Pertanian Bogor Jl. Agatis, Kampus IPB Dramaga 16680
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Journal : Omni-Akuatika

 1 
Maskulinisasi Ikan Nila Melalui Perendaman Larva pada Suhu 36 °c dan Kadar Residu 17a-metiltestosteron dalam Tubuh Ikan Afpriyaningrum MD; Soelistyowati DT; Hardiantho D; Alimuddin Alimuddin; Zairin Jr M; Setiawati M
Journal Omni-Akuatika Vol 12, No 3 (2016): Omni-Akuatika Special Issue Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (674.07 KB) | DOI: 10.20884/1.oa.2016.12.3.132

Abstract

The residue of 17a-methyltestosterone (MT) in fish has been a concern related to food and the environment safety. This study aimed to determine sex ratio of Nile tilapia treated by MT immersion at 36 °C water temperature, and measure the level of testosterone in fish body. Three hundreds of ten-day-old post hatched larvae was immersed in one liter of water containing 2 mg MT, at 26 °C and 36 °C. Immersion was performed for 2 and 4 hours. The results showed that immersion at 36 °C and increasing immersion time increased male percentage. Higher male percentage was obtained in treatment 4 hours immersion at 36 °C (92.5% male) and the effective treatmen 4 hours immersion at room temperature. By ELISA method, testosterone level in MT treated fish decreased sharply to 88.2% at one month after immersion, and being the same as control without MT immersion. Thus, masculization in tilapia could be performed by MT immersion at 36 °C water temperature for 4 hours, and it is most likely that no MT residual in fish after one month post immersion. Keywords: Nile tilapia, sex reversal, temperature, 17a-methyltestosterone
6-Desaturase-Like Encoding Gene Introduction in Catfish (Clarias gariepinus) Anny Hary Ayu; Alimuddin Alimuddin; Dedi Jusadi
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.456 KB) | DOI: 10.20884/1.oa.2017.13.2.188

Abstract

African catfish (Clarias gariepinus) is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce F0 transgenic catfish carrying masou salmon Δ6-desaturase-like (OmΔ6FAD) gene. The Δ6-desaturase enzyme is involved in highly unsaturated fatty acid biosynthesis. Transgenic catfish was produced by sperm-mediated gene transfer using electroporation method. In this study, as the first step, sperms were electroporated with three different OmΔ6FAD concentration (25, 50, and 100 µg mL-1) to have the highest sperm viability after electroporation (125 V/cm, pulse frequency 5 times, pulse length 30 millisecond, pulse interval 0.1 second). The highest sperm viability and sperm carrying OmΔ6FAD were obtain at 100 µg mL-1. This concentration was then used to produce F0 transgenic catfish in the second step. Sperm motility, sperm viability, fertilization rate, hatching rate, and larval survival at 14 days after hatching were the same as the controls (p>0.05). Genomic DNA was extracted from caudal fin and then used as template to identify transgenic F0 by PCR method using specific primer for OmΔ6FAD gene. The PCR result showed that 53.84% of F0 carried OmΔ6FAD gene. The result of fatty acid analysis showed that EPA and DHA contents of F0 transgenic fish and non-transgenic fish were similar. Keywords: catfish, Δ6-desaturase-like gene, fatty acids, electroporation
Growth Performance of Nile tilapia Immersed in 17α-methyltestosterone and rElGH, and Fed a Diet Enriched with rElGH Muhammad Safir; Alimuddin Alimuddin; Mia Setiawati; Muhammad Zairin Junior; Muhammad Agus Suprayudi
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.458 KB) | DOI: 10.20884/1.oa.2017.13.2.288

Abstract

The aim of the present study was to investigate the growth performance of Nile tilapia immersed in 17α-methyltestosterone (MT) and recombinant giant grouper growth hormone (rElGH), and fed a rElGH-enriched diet. A total of 200 tilapia larvae aged 10 days and 14 days post hatching was immersed in 1 liter of 30 g/L saline water for 3 minutes, then in freshwater containing either MT, MT+rElGH, or rElGH for 4 hours. The MT dose was 2.0 mg/L and rElGH was 2.5 mg/L. As control was treatment without MT and rElGH. Fish were maintained in 250-L aquariums for 6 weeks and then moved to net cages for 8 weeks of rearing. The rElGH-enriched diet was fed to the 6-week-old fish for a month, and then fed a non-enriched diet. Each treatment was triplicates. The result showed that the daily growth rate (DGR), biomass gain (BM), survival (SR) and feed (artemia nauplii) consumption during maintenance in the aquariums in the rElGH and MT+rElGH treatments were higher (P<0.05) than in the control. The higher DGR and BM at net cage rearing and lower feed conversion ratio (P<0.05) were also found in MT and MT+rElGH treatments. None of the treatments showed any difference in survival (P>0.05). Profits estimation in the MT and MT+rElGH treatments were 63.38% and 57.91% higher than the control. The present study concluded that higher Nile tilapia farming performance could be obtanied by MT immersion in the larval phase and feeding juvenile on the rElGH-enriched diet.
Transfer of Green Fluorescent Protein (GFP) Gene to Betta splendens Embryos by Transfection and Electroporation Methods Eni Kusrini; Alimuddin Alimuddin; Erma Primanita Hayuningtyas; Syuhada Restu Danupratama
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.692 KB) | DOI: 10.20884/1.oa.2018.14.2.556

Abstract

Transfection and electroporation method shave a high possibility to apply towards transgenic production of small eggs size fish species.  This study aimed to examine the potential of transfection and electroporation methods to use for transferring a foreign gene into betta fish (Betta splendens) embryos using green fluorescent protein (GFP) gene as a model.  Fish were spawned naturally in the ratio of male: female was 1:1, then a total of 200 eggs were taken for each treatment.  Transfection was performed for 30 minutes (room temperature of about 25 °C) at two-cell stage of embryos using transfast reagent.  Transfection reaction consisted of 0.75 µL transfast reagent, 0.25 µL GFP expression vector (DNA concentration: 50 µg/µL) and 99 µL NaCl solution (concentration: 0,95%).  Electroporation was performed using 125 volt cm-1, 3 times pulse frequency at one second interval and pulse length of 7 micro seconds.  A volume of 800 µL GFP expression vector solution (DNA concentration: 50 µg/ µL) in PBS was used for electroporation.  The successful of foreign gene transfer was determined by PCR method with GFP specific primers.  The results showed that hatching rate of eggs in transfection treatment was 67.08%, while the electroporation was 72.09%.  Survival of larvae in transfection treatment was 73.00%, while the electroporation was 75.00%.  The results of PCR analysis showed that transfection method allowed 65% of the survived fish carrying GFP gene, whereas the electroporation method was 70%.  Thus, foreign gene transfer in betta fish can be conducted using the transfection and electroporation methods. 
Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika Abdul Halim Talli Abdul Malik Abyadul Fitriah Abyadul Fitriyah Ade Sunarma Adi Sucipto, Adi Adinda Rizky Tasrianty Afdal Madein Afif Abdurrahman Afpriyaningrum MD Agus Oman Sudrajat Agus Priyadi Ahmad Faisal Ahmad Muzaki Ahmad Ramzi Ghulam Syam Ahmad Zaki Alfiah Mutmainnah Ali Alyaminy, Humaidatul Aminah Zaim Aman Sentosa Panggabean Amin, Muhmmadiyah Andi Aliah Hidayani Andi Haerul Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi, K. Petta Lolo, Arjuni Andi, Maulana K. Andri Gemaini Angela Mariana Lusiastuti Anggriani, Emi Anhar ANNA OCTAVERA Anny Hary Ayu Apriwanto, Indra Aras Syazili Ardo, Okilanda Arief Boediono Arief Eko Prasetiyo Arien Heryansyah Asdar Asep Permana Asep Sopian Asri Ainun Amaliah Atradinal Aulia Asman Aulia Saputra Awi Dassa Ayi Santika Azis Ali Baderiah Baderiah Baderiah Bahri, Nur Alim Benyamin Timotiwu, Paul Bohari Yusuf Boyun Handoyo Budimawan Chaeroni, Ahmad Chaerunnisa Chotban, Sippah DARMAWAN SETIA BUDI Darussalam Darwin, Khadijah DEDI JUSADI Delfian Masrura Deny Sapto Chondro Utomo Desy Nurul Astuti Devi Wardan Sari Dewi Yuniati Dian Hardiantho Dian Hardiantho Diki Nurbaldatun Islam Dinamella Wahjuningrum Dinar Tri Soelistyowati Djadir Dwi Hany Yanti Eko Pramono Emma Suryati Emma Suryati Enang Harris Enang Harris Endi Permata Eni Kusrini Eni Kusrini Erma Primanita Hayuningtyas F.H. Pasaribu Fachruddin Fachruddin Fadhila Maharani Putri Fajar Anggraeni Fatihah, Anariski Fatimah Akbal Fauzan Wahib Alsani Fauziah Zainuddin Firman Firmansyah Fitriadi Fitriyah Husnul Khotimah Fitry Hasdanita Fredinan Yulianda G. Yoshizaki Gemi Triastutik Gloria Ika Satriani Goro Yoshizaki GORO YOSHIZAKI Gunawan Gunawan Gustikasari Amin H. Arfah Hadi Nasbey Hafidah, Riva Halimah Hamda Hamid Habbe Hamzah Hanipa Hardiantho D Harmayani, Ria Harton Arfah Hary Krettiawan Haryanti Haryanti Hasan Nasrullah Hassan, Muhammad Nasiru Herjayanto, Muh. Hisyam Ihsan Ibnu Izzah Idrus Sahar Muhammad Iis Diatin Ika Kartika Ikhwanul Arifan ILHAM Ilham Minggi Imron Imron Indah Mastuti Intan Febrianti Irma Andriani IRMA ANDRIYANI Irmawati, Budimawan Irvan Faizal Irvan Faizal Irwan Irzal Effendi Ita Djuwita Jasmadi Jasmadi JATI, M. SASMITO Jean-Christophe Avarre Joko Santoso Jr., Muhammad Zairin Jubaedah Julie Ekasari Jumrah123 K. Chaitanya Deepti K. Sumantadinata Kadriani, Nur Annisa Kamaruddin Kartika, Ni Made Andry Kartini, Selvira Febriani Katarina Manik, Tumiar Ketut Mahardika Ketut Sugama Khaerullah khairuzzazi Khayati, Sri Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kurniawati, Wd. Intan Leman, Swanggi LESTARI DEWI, AYU Lianasari, Ika Yekti Lidyasari Liza Luky Adrianto M. Zairin Junior M.Y. Jamal Madein, Afdal Maharani, Adinda Mariani, Yuni Marwah, Andi Marwah Mashud MEDIATY Megawati, Novi Mia Setiawati Miftahul Jannah Mira Mawardi MMSI Irfan ,S. Kom Moch. Noer Alim Mochamad Syaifudin Muh Alias L. Rajamuddin Muh. Akmal, Andi Muh. Alias L. Rajamuddin MUHAMMAD AGUS SUPRAYUDI Muhammad Ammar Naufal Muhammad Awal Ramadhan Muhammad Firdaus Muhammad Ikhsan Muhammad Jufri Muhammad Muttaqin Muhammad Sabir Muhammad Safir Muhammad Wawan Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammadiyah Amin Muhram, La Ode MUNTI YUHANA Muslim Muslim Mustakim Mustika Sari, Mustika Musyfikah Ilyas Muzahar N.A. Maswan Nadhirah Nagu Nagu, Nadhirah Nasrullah Nefi Andriana Fajri Nefi Andriana Fajri, Nefi Andriana Ni Made Andry Kartika Nining Asniar Ridzal Noerlitasari Nugraha, Adrianto Fahrizal Nunak Nafiqoh Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur, La Ode Muh.Adam Nur, Rezki Amaliah Nurdin Arsyad Nurfadilla Nurhalija Nurhayati Nurhidayat, Muhammad Nurlaelah Nurlaelah Syarifuddin Nurly Faridah Nurly Faridah Nurul Fadhilah Nurwati Djam’an O. Carman O.Z. Arifin Odang Carman Oman Unju Subandi P. Ratna Kumar Permata Sari, Anggun Petrus Rani Pong-Masak Priatama, Rizki Purwaningsih, Yuli Pustika Ratnawati Putri, Angelina Atika R Rifai R.D. Soejoedono Rahmi, Kurnia Anggraini Ramaita Ratu Siti Aliah Ratu Siti Aliah Reni Agustina Lubis Ridwan RIDWAN AFFANDI Risfiyanty, Dwi Kartika Riswandi Rizky Ayu Fatimah Ridwan Rodliyah Rohayu, Rina Rosidah Rusli RUSTIDJA S. Mariam Samsu Adi Rahman Sani, NurHaslina Sanusi Saputra, Ade Maulana Saputra, Mardepi Sari , Nur Indah Sari Budi Moria Sembiring Sari, Reska Handayani Sarmila Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Shalsyabila , Zabina Shandi Irawan Sholeh Ridwan Siti Aslamyah Siti Fadilah Siti Khodijah Siti Nurul Haliza Sitti Fakhriyyah Slamet Subyakto Soelistyowati DT Soerja Koesnarpadi Soko Nuswantoro Sri Khayati sri murtini . Sri Nuryati Sri Nuryati Srihartini, Yusi St. Fatmawati. L St. Hidayah Triana St. Mukarramah Sukenda . Sukenda Sukenda Sukmawati Ali Sumardin Raupu Sundari Sutamrin Syafrianto, Syafrianto Syuhada Restu Danupratama T. Takeuchi Tahrim, Tasdin Talib, Dr. Ahmad Tasdin Tahrim Tedy Murtedjo Tri Setyo Guntoro Usman Mulbar Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Wa Ode NurRadhiah Ridjalu Wahidah Sanusi Wartono Hadie Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Y. Hadiroseyani Yulintine Yulintine Yuni Mariani Yuni Puji Hastuti Yuningsih, Andri Yusra, Andi Yusuf, Niken Yulian Zairin Jr M Zakki Zainun