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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika
Yuyun Ika Christina
Graduate Program of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Journal : The Journal of Experimental Life Sciences (JELS)

 1 
The Comparison Effect of DOCA and L-NAME on T Cells Activation in the Preeclampsia Mice Model Khodijah, Riska Amalia; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rahayu, Sri; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.03

Abstract

Preeclampsia is a systemic disorder of pregnancy characterized by the maternal immune system's failure to properly adapt to fetal antigens, resulting in chronic inflammatory responses. This research aimed to examine the effects of DOCA (deoxycorticosterone acetate) and L-NAME (Nω-nitro-L-arginine methyl ester) in inducing preeclampsia in pregnant BALB/c mice, focusing on lymphocyte activation. Twenty-four BALB/c mice were randomly divided into four groups: NP (non-pregnant mice), P (healthy pregnant mice), D (pregnant mice received DOCA), and L (pregnant mice received L-NAME). DOCA was dissolved in corn oil and administered subcutaneously at 12.5 mg.kg-1 BW before pregnancy and 6.5 mg.kg-1 BW weekly during pregnancy. L-NAME was administered daily starting from day 5 of pregnancy. Mice were dissected on day 16 of pregnancy, and the spleen was isolated to analyze the relative number of CD4+CD62L+ and CD8+CD62L+ T cells. The results showed that DOCA and LNAME exhibited fewer CD4+CD62L+ and CD8+CD62L+ T cells than healthy pregnant mice. The administration of DOCA at 12.5 mg.kg-1 BW (before mating) and 6.5 mg.kg-1 (day 7 of pregnancy) was more effective in triggering the activation of CD4+CD62L+ and CD8+CD62L+ T cells than the others indicated by the lowest number of CD4+CD62L+ and CD8+CD62L+ T cells. However, the conclusion of which one is more effective in inducing preeclampsia has not been reached. This is due to the many parameters that must be observed related to preeclampsia. Further studies are needed, especially examining proinflammatory cytokine production in T cells after induction with both inducers. Keywords: DOCA, L-NAME, Lymphocyte, Mice, Preeclampsia.
Evaluation of TNF-α and IFN-γ Levels in Macrophage of Preeclampsia Mice Model Induced by DOCA and L-NAME Susanti, Winda Karina; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rahayu, Sri; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.05

Abstract

Preeclampsia is a pregnancy disorder characterized by high blood pressure, proteinuria, and chronic inflammation that potentially leads to multiorgan failure. This study investigated differences in the relative number of pro-inflammatory cytokines (IFN-γ & TNF-α) in preeclamptic mice induced by DOCA and L-NAME. Eighteen pregnant BALB/c mice (n=6): pregnant mice without any treatment (P), pregnant mice treated with Deoxycorticosterone acetate/DOCA (D), and pregnant mice treated with N-ω-L-arginine Methyl Ester/L-NAME (L) and healthy nonpregnant mice as control (NP). DOCA at 12.5 mg.kg-1 BW was injected subcutaneously before mating and 6.5 mg. kg-1 BW after mating every week for 16 days. L-NAME was administered orally at 75 mg.kg-1 BW daily after five days of gestation. Mice were dissected in the second trimester (day 16), and then the spleen was isolated to analyze the relative number of CD11b+IFN-γ+ and CD11b+TNF-α+ using flow cytometry analysis. The results showed that DOCA and L-NAME induction did not significantly induce the production of TNF-α levels in the macrophage of pregnant mice. However, both DOCA and L-NAME significantly induce the production of IFN-γ levels in the macrophage of pregnant mice. Further evaluation of other markers related to preeclampsia should be done to better understand the roles of DOCA and L-NAME in inducing preeclampsia. Keywords: DOCA, L-NAME, mice, preeclampsia, pregnant.
Phaleria macrocarpa Leaves Extract Reduce Tumors Growth and Improve Histological Changes of Liver and Kidney on 4T1 Breast Cancer Mice Model Aminullah, Lela -; Christina, Yuyun Ika; Rifa'i, Muhaimin -; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.02

Abstract

Breast cancer is a type of cancer that highly occurs globally and causes death cases. Of the many ways of treating breast cancer, chemotherapy is the most recommended, even though it causes various disturbing side effects. Therefore, alternative medicine using bioactive compounds of medicinal plants has begun to be widely used, for example, Phaleria macrocarpa, a plant native to Indonesia with anticancer and antioxidant activity. The liver and kidneys are important organs that function to maintain body homeostasis. The use of crude extracts of medicinal plants often causes damage to those organs at inappropriate doses. This research aimed to get an effective dose for reducing breast cancer growth and is safe for the liver and kidneys. A total of 36 mice were divided into six groups, including healthy control, cancer control, cisplatin, and three doses of P. macrocarpa extract (58.9, 117.8, and 235.6 mg.kg-1). The experimental animals were injected using a 4T1 cell line and treated orally using P. macrocarpa leaf ethanol extract for two and three weeks. The tumor volume of mice was measured periodically. At the end of treatment, mice were sacrificed, and their liver and kidney organs were isolated. Both organs were then prepared for H&E staining and observed using a microscope. The results showed that a dose of 58.9 mg.kg-1 and 117.8 mg.kg-1 of P. macrocarpa extract could reduce tumor volume by more than 90%, and the 117.8 mg.kg-1 dose is the safest dose to use because it does not affect the kidney and cause chronic damage to liver tissue. Keywords: extract, kidney, liver, tissue damage, tumor.
Anti-inflammatory Activity of Elephantopus scaber and Sauropus androgynus Combination in Pregnant Mice Infected with Escherichia coli Asfi, Nida; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa’i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.06

Abstract

The current study aimed to investigate the effectiveness of Elephantopus scaber and Sauropus androgynus as anti-inflammatory agents in pregnant mice infected with Escherichia coli. This study used seven treatments group (n=3): K- (healthy pregnant mice), K+ (pregnant mice infected with E. coli), P1 (pregnant mice infected with E. coli + E. scaber 100%), P2 (pregnant mice infected with E. coli + E. scaber 75% and S. androgynus 25%), P3 (pregnant mice infected with E. coli + E. scaber 50% and S. androgynus 50%), P4 (pregnant mice infected with E. coli + E. scaber 25% and S. androgynus 75%), P5 (pregnant mice infected with E. coli + S. androgynus 100%). Flow cytometry analysis was used to analyze cell populations expressing CD4+TNFα+, CD4+IFNγ+ and regulatory T cells (CD4+CD25+CD62L+). All treatment groups significantly (p<0.05) decreased TNFα and IFNγ levels, while the P2 group was more effective in increasing regulatory T cells at the 1st and 2nd trimesters of the pregnancy. This study showed that E. scaber and S. androgynus combination alleviated inflammation by reducing inflammatory cytokines (TNFα and IFNγ) and increasing T-regulatory cells. Therefore, E. scaber and S. androgynus combination could suppress the inflammation during pregnancy and infection. Keywords: Elephantopus scaber, immune system, infection, inflammation, Sauropus androgynous.
Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model Izzah, Fathiyah Nurul; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.01

Abstract

Pulmonary fibrosis is a chronic disease characterized by progressive connective tissue deposition that replaces healthy lung tissue. This study aimed to investigate the effect of Elephantopus scaber L. Ethanol Extract (ESEE) treatment on the relative number of IL-2 cytokine and lymphocyte activation in bleomycin (BLM)-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice were divided into seven treatment groups: N (normal); V or vehicle (corn oil); PF or Pulmonary Fibrosis (BLM 2 mg.kg-1); Dex (Dexamethasone 3 mg.kg-1 + BLM); D1-D3 (ESEE at doses of 0.0504, 0.1008, and 0.2016 mg.kg-1 BW + BLM). ESEE, dexamethasone, and corn oil were administered orally, followed by intraperitoneal bleomycin injection daily for 14 days. Mice were dissected on days 7 and 14, and spleens were isolated to analyze cell populations expressing CD4+IL-2+, CD8+IL-2+, CD4+CD62L+, and CD8+CD62L+. The results showed that bleomycin injection could increase the relative number of IL-2 and decrease the relative number of naive T cells compared to normal mice. ESEE treatment significantly reduced the relative number of IL-2, thus decreasing naive T cell activation after one week of bleomycin injection compared to the mice model. In contrast, the increased IL-2 production led to the increasing naive T cell activation after two weeks of bleomycin injection. Therefore, ESEE treatment has the potential to maintain homeostasis through modulation of IL-2 production and T-lymphocyte activation in the pulmonary fibrosis mice model. Keywords: Elephantopus scaber, IL-2, lymphocytes, mice, pulmonary fibrosis.
Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis Nabilah, Sarah Nahdah; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa’i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.02

Abstract

IL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantopus scaber L. ethanol extract (ESEE) on IL-6 and IL-17 produced by CD4+ and CD8+ in the bleomycin-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice will be divided into seven groups consisting of healthy mice (N), vehicle mice (V), pulmonary fibrosis (PF), Dexamethasone (DEX) as a drug control, and three doses of ESEE (0.0504, 0.1008, and 0.2016 mg.kg-1 BW). ESEE will be administered orally, followed by intraperitoneal bleomycin injection for 14 days. Mice are then dissected on days 7 and 14, and the spleen will be isolated for analysis of the expression of IL-6 and IL-17. The results showed that ESEE effectively reduced levels of IL-6 and IL-17 cytokines produced by CD4+ and CD8+ T cells, and doses three of ESEE (0.2016 mg.kg-1 BW)  (0.2016 mg.kg-1 BW) showed the most effective reduction activity than the Dexamethasone group. The treatment was proven to reduce the expression of IL-6 and IL-17 in mice with a model of pulmonary fibrosis. Keywords: bleomycin, Elephantopus scaber L., IL-6, IL-17, pulmonary fibrosis.
Corrigendum: Evaluating SARS-CoV-2 Spike Protein Transfection in HEK-293T Cells for VLP Applications Rohmah, Ilmiana Nurur; Marlita, Marlita; Kusuma, Kavana Hafil; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Mustikaningtyas, Dewi; Widodo, Nashi; Djati, M. Sasmito
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.01

Abstract

The spike protein of SARS-CoV-2 is crucial for initiating infections by binding to host cells and mediating membrane fusion. In this study, HEK-293T cells were transfected with plasmids encoding three structural proteins of SARS-CoV-2, i.e., Spike (S), Membrane (M), and Envelope (E). This transfection enabled the formation of SARS-CoV-2 Virus-Like Particles (VLPs), which allows for safer studies of the virus and its proteins. To serve as a marker for expression, an enhanced green fluorescent protein (EGFP) was fused to the spike protein, resulting in a Spike-EGFP (S-EGFP) fusion protein. The characteristics of SARS-CoV-2 spike protein expression in the transfected HEK-293T cells were then investigated using polymerase chain reaction (PCR) and flow cytometry. The PCR analysis revealed non-specific DNA band smearing, which did not provide conclusive confirmation of spike protein expression. However, flow cytometry analysis demonstrated that approximately 30% of the transfected cells exhibited green fluorescence, indicating the expression of the Spike-EGFP fusion protein. These findings, obtained through flow cytometry, confirmed the successful spike protein expression in transfected HEK-293T cells.  
Co-Authors Achfas Zacoeb Aminullah, Lela - Annisa, Yuslinda Asfi, Nida Azerlyn, Defiona Rensia Naomi Dewi Mustikaningtyas Dinia Rizqi Dwijayanti Grahadi, Rahmat Izzah, Fathiyah Nurul Kamila, Fairuz Sarah Khodijah, Riska Amalia Kusuma, Kavana Hafil Marlita Marlita, Marlita Minang, Bony Zulkarnaen Moch Sasmito Djati Nabilah, Sarah Nahdah Nafisah, Wirdatun Nashi Widodo Prima, Alex Retno Anggraini Rifa'i, Muhaimin Rifa'i, Muhaimin - Rifa`i, Muhaimin Rifa’i, Muhaimin Rohmah, Ilmiana Nurur Rosyadah, Nuraini SRI RAHAYU Susanti, Winda Karina Turniningtyas Ayu Rachmawati Widyananda, Muhammad Hermawan