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Trioso Purnawarman
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Jurnal Sain Veteriner

 1 
Identifikasi Listeria monocytogenes pada Susu Kambing di Kabupaten Purworejo Jawa Tengah Monika Danaparamitha Andriani; Trioso Purnawarman; Retno Damayanti; Syafril Daulay
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (989.878 KB) | DOI: 10.22146/jsv.22809

Abstract

Listeria monocytogenes is a pathogenic Listeria species, especially for high-risk groups and it that can be transmitted through contaminated food. Goat milk produced by traditional milking process and storage has a high risk of contamination. The aim of this study was to identify the presence of L. monocytogenes in fresh goat milk in Puworejo regency, Central Java. This study used 60 samples of raw goat milk that were obtained from seven farms by disease detection sampling method. All of the used method in this research refer to Indonesian National Standard (SNI) ISO 11290-1: 2012 about Microbiology of food and feed for detection and enumeration of Listeria monocytogenes. A total of 60 samples of raw goat milk that used in this study were not contaminatedwith L. monocytogenes. Based on the results of this study, it can be concluded that all the samples of raw goat milk were free from L. monocytogenes and have fulfilled the Indonesian National Standard (SNI) ISO No. 7388: 2009 about Limit of Microbial Contamination in Food.
Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat Dwida Agustina Suherman; Etih Sudarnika; Trioso Purnawarman
Jurnal Sain Veteriner Vol 41, No 2 (2023): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.83050

Abstract

Resistansi antibiotik atau sering disebut sebagai pandemi senyap, merupakan satu diantara penyebab masalah kesehatan global yang harus diwaspadai. Susu segar merupakan media pembawa yang mudah terkontaminasi bakteri, salah satunya ialah bakteri E. coli. Antibiotik yang sering dipakai dalam pengobatan terhadap sapi adalah penisilin yaitu antibiotik golongan betalatam yang memiliki kemampuan membunuh bakteri dengan mencegah pembentukan protein dinding sel. Tujuan penelitian ini adalah untuk mendeteksi keberadaan dan prevalensi E. coli pada susu segar, serta menguji resistansinya terhadap antibiotik penisilin. Sebanyak 75 sampel susu kandang dipilih secara acak, berasal dari 6 kelompok ternak sapi perah dan dikumpulkan oleh masing-masing petugas lapang. Uji identifikasi keberadaan E. coli mengacu kepada Standar Nasional Indonesia (SNI) nomor 01-2897-2008 tentang metode pengujian cemaran mikroba pada susu, daging dan telur. Isolat bakteri yang teridentfikasi E. coli dilaukan pengujian resistansi terhadap antibiotik penisilin menggunakan metode Kirby-Bauer disk diffusion dengan penentuan standar berdasarkan Clinical and Laboratory Standards Institute (CLSI). Wawancara terstruktur dilakukan kepada 75 peternak yang diambil sampel susu kandang. Data yang diperoleh dianalisis secara deskriptif. Hasil identifikasi menunjukkan bahwa bakteri E. coli yang terdeteksi pada sampel susu kandang sebanyak 24/75 isolat (32%). Hasil uji resistansi menunjukkan bahwa sebanyak 23/24 isolat E. coli (96%) resistan terhadap antibiotik penisilin. Kesimpulan dari penelitian ini E. coli yang berhasil diisolasi dari susu kandang di wilayah KPSCU telah resistan terhadap antibiotik penisilin, sehingga dibutuhkan penerapan praktik higiene sanitasi, pengawasan penggunaan antibiotik, peningkatan pengetahuan peternak dan petugas kesehatan hewan terkait penggunaan antibiotik di lapangan. 
Safety Risk Factors of Duck Meat Sold in Bogor City annisa, rizka fajri; Purnawarman, Trioso; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.101423

Abstract

With the increasing demand for duck meat in Bogor City, this poses a risk of the presence of pathogenic bacteria throughout the food supply chain. Traditional markets are one of the places that have a high risk of contamination with pathogenic bacteria that can endanger public health. This study aims to determine the presence of Salmonella and E. coli in duck meat sold in traditional markets in Bogor City and the risk factors that influence the presence of these pathogenic bacteria. This study was a cross sectional study with a total of 45 duck meat samples obtained from 3 traditional markets in Bogor City. Samples were taken to the SKHB IPB Veterinary Public Health Laboratory for microbiological testing. Risk factors that may affect the safety of duck meat were assessed using a questionnaire. The results showed that 13 samples (29%) were positive for Salmonella and 27 samples (60%) were positive for E. coli. The presence of Salmonella and E. coli in duck meat indicates cross-contamination during slaughter, evisceration and poor sanitation and hygiene practices. Continuous monitoring and reporting of the presence of Salmonella and E. coli is needed so that duck meat consumed by the public is safe for consumption.
Co-Authors A.S. Satyaningtijas Afiff , Usamah Agus Setiyono Agustin Indrawati Aji Barbora Niasono Amalo, Gabryella Fransina Anisa Rahma, Anisa annisa, rizka fajri Aulia Andi Mustika Ayunina, Yasmine Qurrota Chairun Nisa Connie Januari Debby Fadhilah Pazra Denny Widaya Lukman Dwi Desmiyeni Putri Dwida Agustina Suherman Engki Zelpina Etih Sudarnika Fachriyan Hasmi Pasaribu Gabryella Fransina Amalo Hadiyanti, Cresensia Rara Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Herwin Pisestyani I wayan Teguh Wibawan IETJE WIENTARSIH Joko Pamungkas Karunia Maghfiroh Khoirun Nisa Kumala Andri Asari Lina Noviyanti Sutardi Madyastuti, Rini Maulita Lutfiani Melani Wahyu Adiningsih Mohamad Syamsul Ma’arif Monika Danaparamitha Andriani Muharam Saepulloh Nugroho, Radhitya Aryo Okti Nadia Poetri Rahmat Setya Adji Retno Damayanti Soejoedono Rusman Efendi Santosa, Alfian Sa’diah, Siti Septa Walyani Sudarwanto, Mirnawati Bachrum Surachmi Setiyaningsih Susanto Nugroho Syafril Daulay Syahdu Pramono Tarigan, Ronald Vindriati, Zukhrufa Vista Yasmine Qurrota Ayunina