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Journal : Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)

Identification of determining factors size and shape Simbal cattle and Brahman Cross cattle in Pamenang Barat Merangin district Fitra Diansah; Depison Depison; Silvia Erina
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 2 (2021): August 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.02.08

Abstract

This study aims to determine the determinants of size and shape of Simbal and Brahman Cross cattle in the West Pamenang sub-district, Merangin district. The method of this study was a survey with a purposive sampling technique. Sampling criteria included: Sample of each breeds consisted of 60 Simbal and 60 Brahman Cross cattle aged 1-2 years. Data observed included: body length, shoulder height, chest circumference, inside chest, chest width, canon circumference, hip height, body weight, and body weight gain. The differences in body measurements between Simbal and Brahman Cross cattle analyzed by T-test. Identification of the determinants of size and shape of Simbal and Brahman Cross cattle were analyzed using Main Component Analysis method. The results of this study were body weight, body weight gain, and body measurements of Simbal cattle were significantly different (P <0.05) higher than Brahman Cross cattle, both male and female. Simbal cattle and male Brahman Cross cattle were significantly different (P <0.05) higher than females. The conclusion shows that body weight, body weight gain, and body measurements of Simbal cattle are higher than Brahman Cross cattle. Male body weight gain was higher than females in both Simbal and Brahman Cross cattle. The determining factor for the body size of Simbal and Brahman Cross cattle is chest circumference, while the determining factor for body shape of Simbal cattle is the height of shoulders, while Brahman Cross is body length. The highest correlation between body measurements and body weight in Simbal and Brahman Cross cattle, both male and femal is chest circumference (LD).
Association of Gene Growth Hormone (GH) Diversity with Quantitative Characteristics of Crossing Merawang Chickens with Arabic Chickens Muhammad, Mus'ab; Depison; Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.2

Abstract

The growth hormone (GH) gene plays a crucial role in regulating the growth and metabolism of Chickens. This study investigates the polymorphism of the GH gene and its association with growth traits in chickens resulting from the crossbreeding of Merawang and Arabs (MeA). The research began by raising 60 chickens—30 males and 30 females—from the Merawang and Arabic chicken cross for four months. Various growth traits, including weight, weight gain, and body size, were measured. Blood samples from the MeA chickens were collected for DNA analysis to assess GH gene polymorphism. The analysis involved DNA extraction, PCR amplification, and RFLP using the Taql enzyme. The data were analysis using T-test to evaluate the association of the GH gene polymorphism with growth traits. The results showed that the male of crosses between Merawang and Arab chicken (MeA) have higher significant (P<0.05) growth traits, including body weight, weight gain, and body size than female MeA chickens. The chest circumference is a defining characteristic of body size in male and female MeA chickens, while the upper body length is a crucial feature of body shape. The GH gene were found polimorpic with three genotypes, including GG, AG, and AA. The GH gene polymorphisms have a significant association (P<0.05) with growth traits, including body weight, body weight gain, and body size. Chicken with GG genotype have a higher growth trait than others. The GH gene can be used as a potential genetic marker for selection for improving chicken growth.
The Relationship Between the Diversity of Growth Hormone Genes and the Body Weight of Sentul Chickens Pratama, Shera Aditia; Depison; Gushairiyanto, Gushairiyanto
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.05

Abstract

This study aims to determine the diversity of growth hormone genes in Sentul chickens using PCR-RFLP. This study used 70 samples of Sentul chicken blood (35 males and 35 females). The research methods are Bodyweight and body weight gain data collection, bloodcollection, DNA extraction, and PCR-RFLP. Polymorphism analysis included genotype and allele frequencies, Hardy-Weinberg balance, and heterozygosity. The difference in the average body weight against the genotype of the GH|Msp1 gene was tested for the average difference (t-test). The average body weight of DOC - 3 months and body weight gain of DOC - 3 months in male Sentul chickens were significantly different (P <0.05) higher than female Sentul chickens. The GH|MspI gene in Sentul chickens is polymorphic. The Sentul chicken population was in Hardy-Weinberg equilibrium. Heterozygosity in the Sentul chicken population was Ho 0.228, greater than the He value of 0.213. Genotypes (++) and (+-) were significantly different (P<0.05) on body weight and body weight gain of Sentul chickens aged DOC - 3 months. The conclusion of this study, the allele frequency at the GH|MspI Sentul chicken is polymorphic with two genotypes, namely (++) and (+-), and there are two kinds of alleles (+) and (-). Chisquare test results on the GH|mspI Sentul chicken show balance Hardy-Weinberg. Heterozygosity in Sentul chickens showed diversity. The genotype of the GH|Msp1 Sentul chickens with the genotype (++) had higher body weight and body weight gain than those with the (+-) genotype
Growth Hormone Gene Diversity and Quantitative Characteristics of Thin-Tailed Sheep in Muara Tembesi District Batang Hari Regency Pratama, Bondan; Depison, Depison; Gushairiyanto, Gushairiyanto; Gunawan, Asep; Sumantri, Cece
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.13

Abstract

Research on thin-tailed sheep (TTS) via quantitative and molecular characterization is essential for obtaining primary data on genetic diversity. The quantitative description includes body weight, weight gain, and body measurements. Molecular characterization is performed directly on structural genes. One gene that affects livestock growth and metabolism is the growth hormone (GH) gene. The commonly used method is PCR-RFLP. The research was conducted in two stages. Field research was conducted to obtain quantitative characteristic data, including body weight, weight gain, and body measurements, as well as blood samples from 62 TTS (31 males and 31 females). Research in the laboratory includes DNA extraction, PCR amplification, and restriction with the Msp1 enzyme. Data analysis included a t test to calculate the differences in body weight, weight gain, and body size between male and female TTS. T2-hotelling was used to compare the morphometric body sizes of the male and female TTS simultaneously, and principal component analysis was used to determine the body shape and size characteristics of the male and female TTS. The results revealed that the male TTS mean body weight, weight gain, and body size were significantly (P<0.05) greater than those of the female TTS. The results of the molecular analysis of the GH gene with the MspI cutting enzyme revealed that it is monomorphic with the ++ genotype. Conclusion: Body weight, weight gain, and body size were greater in males with TTS than in females with TTS. The body size characteristics of male and female TTS patients were body length, shoulder height, and chest circumference. The body shape characteristic was chest depth. The restriction of the GH|Msp1 TTS gene DNA fragment in exon 5 in the Muara Tembesi district is monomorphic.
Co-Authors ., GUSHARIYANTO A. S. Wilastra Abdul Azis Abdul Azis Ade Octavia Adelia, Siska ADRIANI ADRIANI Adriani Adriani Adzitey, Frederick Afriani Afriani Agus Susilo Ahmad Taofik Almakmum, Husni Amalia Fildzah Ghassani Anak Agung Istri Sri Wiadnyani Anie Insulistyowati, Anie Annisa Rianti Ardi Novra Ardiantoro, Ari Arfiana, BS Monica Asa Bela Sri Reformasi Nala Putri Asa Bela Sri Reformasi Nala Putri Asep Gunawan Avrilliani, Puspita B., SUPRIAWAN Bawah , Juliana Bayu Rosadi Bella Pratiwi Putri Berliana Berliana Berliana Berliana Cece Sumantri Dea Tri Ananda Dika Irmaya E. Wiyanto Eko Wiyanto Eko Wiyanto Eko Wiyanto, Eko Endri Musnandar Erina, S. Ester Restiana Endang Gelis Fauzan Ramadan Fetty Febriana Rahayu Fitra Diansah Gushairiyanto Gushairiyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto, Gushariyanto H. Ediyanto H. Suryani Haris Lukman Hariski, M Helmi Ediyanto Heru Handoko Heru Handoko Hidayat, Gradiandri Hosen, Tanjir Hossain, Md. Murad Husni Almakmum Irmaya. H, Dika Islam, Nazmul Jalius Jalius L. H. M. Gultom Lisna Lisna Lisna Lisna, Lisna Lisna, Lisna M., SAMIUN Mithun, Mahmudul Hasan Monten, Stephen K. Kanten Muhammad Abdu Muhammad, Mus'ab Muqsita Rahmat Nabilah Ika Puteri Nasution, Annio Indah Lestari Nelwida Nelwida Nurhayati Nurhayati Nurul Huda Pakpahan, Roberyo Pratama, Bondan Pratama, Shera Aditia Prayoga Alfano Putri, Asa Bela Sri Reformasi Nala R. A. Muthalib Ratna Sholatia Harahap Redo Prawira Rhaphon Seprian. Ht Riris Roiska Rizky Janatul Magwa S. Erina Salsabila Salsabila Septy Heltria Setiyawan, Khoirudin Anton Shalsabila Novia Al-Sobri Sidik, Wahid Hasyim Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Sofi Crisdayanti Suyadi Suyadi Talukder, Md. Khyrul Alam Triana Mardiah Utama, Millisani W. P. B. Putra Wahidu Zzaman Wahyuni Wahyuni Widya Pintaka Bayu Putra Wiwaha Anas Sumadja Y. Alwi Yun Alwi Zafitra A.