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All Journal Jurnal Sains dan Seni ITS IPTEK The Journal for Technology and Science Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology AKSES: Jurnal Ekonomi dan Bisnis Jurnal Florea Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Transportasi Laut Ekosiana : Jurnal Ekonomi Syariah Journal of Marine-Earth Science and Technology Sewagati Rumphius Pattimura Biological Journal English Language and Literature in Education Journal (ELLINE Journal) Medical-Surgical Journal of Nursing Research Khidmah Nusantara Syaikhuna: Jurnal Pendidikan dan Pranata Islam STAI Syaichona Moh. Cholil Bangkalan Paradigma: Jurnal Filsafat, Sains, Teknologi, dan Sosial Budaya
Dewi Hidayati
Institut Teknologi Sepuluh Nopember
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Journal : Rumphius Pattimura Biological Journal

 1 
DNA Barcoding Application Using CYTB (Cytochrome-B) Marks for Authentication of Patin Fish (Pangasius sp.) Products in Surabaya Area Aulia, Wina Intan Nur; Setiawan, Edwin; Triwibowo, Radestya; Saputro, Triono Bagus; Hidayati, Dewi; Nurhayati, Awik Puji Dyah; Ridho, Muhammad Ainur Rosyid
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p079-092

Abstract

DNA barcoding is a practical and accurate molecular technique for identifying processed fish products that lack morphological characteristics. However, its application is limited by the availability of reference databases, DNA quality, and primer suitability. This study aims to determine the genetic identity of pangasius catfish (Pangasius sp.) marketed in Surabaya using the Cytochrome b (CytB) gene and to evaluate PCR-RFLP as an alternative to sequencing. A total of twenty samples (fresh and frozen fillets) were analyzed through DNA extraction and PCR amplification of the CytB gene, followed by in silico and in vitro analyses.The results show that CytB demonstrates high amplification success and provides sufficient resolution for species-level identification based on Neighbor-Joining analysis with the Kimura 2-parameter model. The study confirms that the samples were genetically identified as Pangasius pangasius and Pangasius hypophthalmus. This research highlights the novelty of integrating in silico prediction with laboratory validation to improve marker evaluation in DNA barcoding studies. Furthermore, it demonstrates that CytB-based PCR-RFLP is a rapid and cost-effective alternative to sequencing for detecting species mislabeling. Overall, the CytB gene is proven to be a reliable molecular marker for species authentication, supporting traceability systems and strengthening seafood authenticity monitoring in commercial markets.
Co-Authors A, Aunurohim Abdul Razak Abdul Hadi, Abdul Razak Achmad Fauzi Afrilya Dina Salsabila Alifia Cahyaning Putri Anak Agung Gede Sugianthara Anies Wijayanti Anies Wijayanti Anis Azimah Aulia Istighfarotunnisa Aulia, Wina Intan Nur Aunurohim Aunurohim Awik Pudji Diah Nurhayati Awik Puji Dyah Nurhayati Bahruddin Salam Danang Samudra Devi Pramesti Wulandari Didit Prasetyo Dini Adni Navastara, Dini Adni Dwi Oktafitria Edwin Setiawan Eko Purnomo Enny Zulaika Enny Zulaika F, Fauziah Farid Kamal Muzaki Fitria, N. Yeni Geoloveriana Esasi Handoko, Wisnu Hani Sirine Hermawan Hermawan Hermawan Iska Desmawati Kamila Islamiati Kharisma Armida Ulayya Kristianto Kristianto Kuntum Febriyantiningrum Lanny Kartikasari Lissa Rosdiana Luluk Anjarwati Maitsa Adzro Fania Marita Ika Joesidawati Millah, Ro’iqotul Mulyadi, Yeyes N. Nurjannah Nadhila, Auliya Rahma Nadila Wulan Cahyani Natanael Hari Wijaya Nia Nurfitria Noor Nailis Sa'adah Noor Nailis Saadah Noor Nailis Sa’adah Nova Maulidina Ashuri Nurjannah N, Nurjannah Nurlita Abdulgani Nurlita Abdulgani Nurul Jadid Oktafitria, Dwi Rahmadani, Syakirah Shafwah Rahmat Rarastoeti Prastiwi Ridho, Muhammad Ainur Rosyid Rony Irawanto S, Suwarsih Sa'adah, Noor Nailis Sabila Ghassani Salim Arrokhman Saptarini, Dian Setiawan, Ariyono Shabrina Syifa Ghaissani Shinta Hartanto Shinta Hartanto, Shinta Shovitri, Maya Sri Wulandari Sukardiman Sukma, Nur Awaliyah Mentari Syahroni, Nur Triono Bagus Saputro, Triono Bagus Triwibowo, Radestya Tutik Nurhidayati Utami, Prastyo Rinie Budi Wahyuni, A. A. Istri Sri Wijayanti Pujitono Zahid, Achmad Zufar Azzaria Azmi Zuwanita, Cyntya Sri