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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Crop Science

Iswari Saraswati Dewi

Balai Besar Bioteknologi dan Sumberdaya Genetika Pertanian Jl. Tentara Pelajar No. 3A Bogor 16111, Indonesia

Author-ID : 252633

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Analysis of drought stress tolerance in doubled haploid lines of green super rice at the vegetative stage Nurhidayah, Siti; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Lubis, Iskandar; Hadianto, Wira; Munandar, Arief
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.63893

Current climate change has caused drought in various regions, which has decreased rice yields. Green super rice (GSR) has the characteristic of being tolerant to water limitation. GSR lines were bred through anther culture technique to obtain doubled haploid (DH) plants. This study aimed to analyze the response of DH GSR lines to drought stress at the vegetative stage and identify potential lines based on the weighted selection index. The experiment was arranged in a randomized complete block design with three replications. The materials used consisted of twenty DH lines, Inpari 42 Agritan GSR, and Inpari 18 as check varieties, Salumpikit as a drought-tolerant check, and IR 20 as a drought-sensitive check. Qualitative data were analyzed using the non-parametric Friedman test. The result showed different responses among the lines for leaf rolling, leaf drying, and recovery ability. Line SN14 exhibited moderate leaf drying and showed improvement to mild tolerance during the recovery phase. Nine lines (SN12, 14, 32, 40, 51, 57, 58, 59, and 60) were selected using a selection index based on leaf rolling, leaf drying, and recovery ability. These lines can be further tested for drought tolerance tests until the reproductive stage, and the tolerant lines could be useful for future development. Keywords: abiotic stress; anther culture; climate change; selection index; water limitation

Konstribusi Akumulasi Silikat, Nitrogen dan Aluminium terhadap Ketenggangan Aluminium dan Ketahanan terhadap Penyakit Blas pada Padi Gogo Bakhtiar, ,; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Aluminum (Al) toxicity and blast disease are the most important yield-limiting factors for upland rice production in acid soils. The objective of this experiment was to examine the contribution of accumulation of Silicate (Si), Nitrogen (N) and Al in plant tissue on Al tolerance and blast disease resistance in upland rice. The experiment was arranged in a split-split plot design with 2 replications. Main plots were randomly assigned to blast treatment (control and inoculation to blast fungi). Subplots were assigned to control box (lime 1.5 AlEC) and another acid soils (no lime) box and sub-subplots were assigned to the tested genotypes. The result of the experiment showed that leaf blast disease resistance in rice cannot be solely explained by Si or N content in shoot tissue. The resistant to leaf blast disease might be attributed by high ratio Si/N weight in shoot. Al tolerance was ascribed by low reduction in root growth, high shoot dry weight, high Si content in shoot, and as well as high of Si/Al ratio in root. Key words: Al-tolerance, blast disease, upland rice, Si/Al ratio

Deteksi Dini Toleransi Padi Hibrida terhadap Kekeringan menggunakan PEG 6000 Afa, La Ode; Purwoko, Bambang Sapta; Junaedi, Ahmad; Haridjaja, Oteng; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this study was to determine a selection method at early stage for drought tolerant hybrid rice. Theexperimental design was split plot with 3 replications. In the first experiment the main plot was concentration of PEG 6000consisting of control and concentration 25% of PEG 6000. The subplots were hybrid genotypes/varieties, i.e. BI485A/BP3,BI485A/BP5, BI485A/BP10, BI485A/BP12, BI485A/BP15, BI599A/BP5, BI599A/BP15, BI665A/BP6, Maro, Hipa 8, IR64and Limboto. In the second experiment the main plot was drought stress level which consisted of control and drought stressat 60% field capacity. The subplots were hybrid genotypes/varieties used in the first experiment. The results showed that PEG 6000 could be used to detect drought tolerant genotypes at early stage. Seedling dry weight was the major character for selecting drought tolerant genotypes. Genotypes BI485A/BP15 and BI559A/BP15 are tolerant to drought and can potentially be grown in rainfed lowland.Keywords: drought tolerant, early selection, hybrid rice, polyethylene glycol

Konservasi In Vitro Pamelo (Citrus maxima (Burm.) Merr.) melalui Pertumbuhan Lambat Tyas, Kartika Ning; Susanto, Slamet; Dewi, Iswari Saraswati; Khumaida, Nurul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Indonesia is one of the countries which have abundant germplasm of pummelo (Citrus maxima (Burm.) Merr.). Thepummelo germplasm must be conserved to prevent its extinction due to biotic and abiotic stresses. In vitro conservationusing slow growth technique can be considered as an alternative of ex-situ conservation. Two experiments were conductedto obtain suitable medium to conserve pummelo in vitro. The first experiment was conservation using modified concentrationof MS and sucrose. MS medium concentrations were 1/2MS and MS, while sucrose concentrations were 0, 1, 2 and 3%. Thesecond experiment was conservation using osmoticum and retardant in MS medium. There were six combinations of MSmedium supplemented with osmoticum and retardant, i.e. MS + sucrose 3%, MS + sucrose 3% + paclobutrazol 7.5 ppm, MS+ sucrose 3% + paclobutrazol 15 ppm, MS + sorbitol 2%, MS + sorbitol 2% + paclobutrazol 7.5 ppm, MS + sorbitol 2% +paclobutrazol 15 ppm. The results showed that reducing MS medium and sucrose consentration decreased leaf number andshoot length but increased root number and length. The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length. Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.Keywords: Citrus maxima, osmoticum, preservation, retardant, slow growth

Potensi dan Stabilitas Hasil, serta Adaptabilitas Galur-galur Padi Gogo Tipe Baru Hasil Kultur Antera ., Purbokurniawan; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe objectives of the research were to obtain information on yield potential, adaptability and stability of the upland rice lines. Ten lines and two cultivars were planted at five different locations in November 2010-March 2011. In each location, the experimental design was randomized complete block design with four replications. Observation was done on grains weight per hectar. The results showed that FM1R-1-3-1 achieved the highest productivity (5.65 ton ha-1). Genotype FG1R-36-1-1 was classified as stable genotype by four yield stability analysis as followed: Francis-Kannenberg, Finlay-Wilkinson, Eberhart-Russell and AMMI. Genotypes FG1-70-2-1, FG1R-30-1-5, FG1R-30-1-4 and FG1R-30-1-3 were classified as stable genotypes by three yield stability analysis: Finlay-Wilkinson, Eberhart-Russell and AMMI. AMMI analysis showed that FG1-6-1-2, FG1-65-1-2, FG1R-30-1-1, FM1R-1-3-1, Fat-4-1-1, Situ Bagendit and Towuti as specific genotypes in certain environment.Keywords: dynamic, new plant type, specific, static, yield

Penampilan Agronomi dan Uji Toleransi Naungan Galur Dihaploid Padi Gogo Hasil Kultur Antera Mara, Kartika Kirana Sangga; Purwoko, Bambang Sapta; ,, Eko Sulistyono; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTShifting of upland rice cultivation to marginal land faces drought stress, while planting under young plantation faces shading stress. There should be lines or varieties with good agronomic character and tolerant to the shading stress. Lines used in this experiment were obtained from anther culture, i.e., III3-4-6-1, I5-10-1-1, WI-44, GI-7, O18-b-1, IW-67, IG-19, IG-38, IW-56, B13-2-e. Two experiments were conducted, i.e., agronomic performance experiment and shading tolerance experiment with a quick test method at seedling phase. Batutegi and Way Rarem were used as check varieties in the agronomic experiment. In shading tolerance experiment, Kalimutu was used as sensitive check and Jatiluhur was tolerant check varieties. The results of experiment indicated that B13-2-e (4.64 ton ha-1) and WI-44 line (4.05 ton ha-1) dihaploid lines showed high grain yield and comparable to Way Rarem (4.95 ton ha-1). B13-2-e and WI-44 lines were tolerant to shading. Another tolerant lines to shading, i.e., I5-10-1-1, O18-b-1, and IW-56. B13-2-e and WI-44 lines were recommended to advanced verification on multiple cropping system.Keywords: dry land, multiple cropping

Penampilan Galur Harapan Mutan Dihaploid Padi Tipe Baru di Sulawesi Selatan Dewi, Iswari Saraswati; Lestari, Endang Gati; ,, Chaerani; Yunita, Rossa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTSouth Sulawesi is known as one of national rice production centers. However, average productivity of rice varieties planted in that area (4.43 ton ha-1) is lower than those of rice productivity in Java (5.25 ton ha-1). The aims of this research were to evaluate agronomic characters and adaptation of 7 dihaploid mutant advanced lines of new plant type (DH-NPT) of rice at several locations in South Sulawesi. The research was conducted in 2012 at Maros, Gowa, Barru, and Pangkep. The experiments were conducted in randomized complete block design with 3 replications nested in locations. Treatment consisted of 7 DH-NPT of rice, i.e., BIO-MF115, BIO-MF116, BIO-MF125, BIO-MF130, BIO-MF133, BIO-MF151, BIO-MF153, and control varieties i.e., Fatmawati, Ciherang, and Inpari13. The results indicated that in general the lines had medium height (102.77-110.23 cm), moderate productive tiller (9-16 tiller per hill), moderate days to flower (50%), i.e., 73-76 days after sowing (DAS), earlier days to harvest (103-110 DAS), moderate panicle length (28.35-29.31 cm), large number of grain per panicle (> 250 grains) with moderate panicle fertility (63-70%), moderate 1,000 grain weight, i.e., 26.51-27.75 g, and high yield (7.51-8.09 ton ha-1). Four lines, i.e., BIO-MF116, BIO-MF130, BIO-MF151, and BIO-MF153 were stable and had wide adaptability. Other lines, i.e., BIO-MF125 and BIO-MF133 were sensitive to environmental changes, therefore they were classified as specifically adapted to favorable environment; while BIO-MF115 was not sensitive to environmental changes, and therefore it was adapted to non-favorable environment.Keywords: adaptation, agronomic characters, rice mutant

Kultur Antera untuk Mendapatkan Galur Padi Toleran Salinitas Safitri, Heni; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTHaploid breeding through anther culture allows shortening of the breeding cycle and production of homozygous lines from a segregating population in the immediate generation. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of twelve F1s, derived from reciprocal crossing between high yielding rice variety and rice tolerance to salinity, through anther culture. Completely randomized design with 20 replications was used in this research. Medium for callus induction was based on N6 medium + 2.0 mg NAA L-1 + 0.5 mg kinetin L-1 + 1 mM putrescine, while regeneration medium was based on MS + 0.5 mg NAA L-1 + 2.0 mg kinetin L-1 + 1 mM putrescine. Rooting were done in MS medium + 0.5 mg IBA L-1 + 1 mM putrescine. The result indicated that F1 derived from IR77674/Inpari 29 (3.1% green plants/total anther) was the most responsive genotypes in rice anther culture (high anther culture ability). After greenhouse grow out 125 putative double haploid plants were obtained (41.5% from total acclimated green plantlets). Keywords: double haploid, green planlets, indica rice, salt tolerance

Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah Gunarsih, Cucu; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Syukur, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing: INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM putresin, and media for rooting was based on MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%, respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants), G5 (21 plants), and G6 (26 plants).Keywords: Callus induction, doubled haploid, rice

Pemilihan Karakter Agronomi untuk Seleksi pada Galur-galur Padi Dihaploid Hasil Kultur Antera Akhmadi, Gerland; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, dan Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Anther culture technique is able to accelerate plant breeding activities. The objectives of this research was to determine the agronomic traits that could be used for selection of the dihaploid rice line population through the calculation of heritability, genotypic correlation, path analysis and selecting dihaploid rice lines. The plant material used was 65 dihaploid rice lines DH 1 of F1 anther culture plants and Ciherang and Inpari 13 as check varieties. Agronomic characters that could be used as selection character are generative plant height, number of filled grain per panicle, the total number of grains per panicle, weight of 1,000 grains, and grain per hill. Twenty three dihaploid lines were selected based good agronomic characters with criteria generative plant height between 80-120 cm, number of filled grains per panicle > 100, number of grains per panicle > 120, weight of 1,000 grains > 20 g, and grain per hill > 25 g.Keywords: anther culture, heritability, agronomic characters

Co-Authors ,, Mawaddah AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Awang Maharijaya Bakhtiar, Bakhtiar. Bambang Sapta Purwoko Chaerani ,, Chaerani Cucu Gunarsih da Cunha, Rojino Danu Kuncoro Desta Wirnas Eko Sulistyono Endang Gati Lestari Eny Widajati Heni Safitri Iskandar Lubis Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartika Ning Tyas Muhamad Syukur Munandar, Arief Nindita, Anggi Nita Kartina, Nita Nurul Khumaida Oteng Haridjaja Purbokurniawan . Purwoko, Bambang Sapta Purwoko Purwoko, Bambang Sapto ROSSA YUNITA Safitri, dan Heni Sani, Mutiyara Sintho Wahyuning Ardie Siti Nurhidayah Slamet Susanto Sugiyanta, dan Trikoesoemaningtyas Willy Bayuardi Suwarno Wira Hadianto, Wira

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