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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Crop Science

Iswari Saraswati Dewi

Balai Besar Bioteknologi dan Sumberdaya Genetika Pertanian Jl. Tentara Pelajar No. 3A Bogor 16111, Indonesia

Author-ID : 252633

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Karakterisasi Sifat Agronomi Tanaman Padi Beras Merah Dihaploid Berpotensi Hasil Tinggi Diperoleh melalui Kultur Antera Antera ,, Mawaddah; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, dan Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Observation and identification of rice germplasm with advanced traits are important activities in the process of obtaining a variety of rice because every genotype will show unique characters. Genotype derived from breeding program as anther culture need to be characterized to identify the doubled haploid lines that are potential to be developed in red rice breeding. This research was aimed to obtain agronomic characters of doubled haploid lines of red rice. Materials used were 59 genotypes, i.e., 57 doubled haploid (DH0) lines with red grain and 2 check varieties (Inpari 24 and Aek Sibundong) as control. The experiment used a randomized complete block design with three replications. The results exhibited there was agronomic variation among DH0 of red rice lines. Variance analysis showed that genotype affected most characters, except for grain dry weight per hill. Plant height, number of productive tiller, harvesting age, panicle length, number of grains per panicle, and number of filled grain per panicle can be used as a reference for effective selection to develop high productivity of red rice variety.

Heritabilitas, Karakterisasi, dan Analisis Clustergram Galur-galur Padi Dihaploid Hasil Kultur Antera Anshori, Muhammad Fuad; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, Sintho Wahyuning; Suwarno, Willy Bayuardi; Safitri, dan Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.

Penentuan Indeks Seleksi Toleransi Kekeringan Galur Dihaploid Padi Sawah Tadah Hujan pada Fase Perkecambahan Akbar, Miftahur Rizqi; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, dan Willy Bayuardi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Rainfed rice tolerant to drought and high yielding would be an alternative to rainfed lowland areas prone to drought stress. Selection in the early phase of plant growth will accelerate the effort to obtain rainfed varieties. The objective of this experiment was to identify characters that significantly affect the selection of drought tolerance using PEG 6000 concentration of 25% in the germination phase. The experiment was conducted in greenhouse of ICABIOGRAD, Bogor, using a factorial randomized complete block design with three replications. The first factor was 32 rice genotypes while the second factor was 2 levels of PEG 6000, i.e., concentrations of 0 and 25%. The results showed that the correlation analysis and principal component analysis obtain the important characters namely the germination percentage, seminal root length, and the dry weight of seminal roots. Based on discriminant analysis, the index matching value of 96.77% was suitable to distinguish tolerant and sensitive genotypes using PEG 6000 concentration of 25% in the germination phase.

Skrining Awal Toleransi Galur-galur Dihaploid Padi Gogo terhadap Cekaman Kekeringan pada Stadia Bibit Kartina, Nita; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, Desta; Nindita, dan Anggi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Development of doubled haploid lines of upland rice through anther culture is one way to obtain superior upland rice varieties tolerant to drought. The objectives of this research were to determine the response of doubled haploid lines to drought stress at seedling stage and to select tolerant doubled haploid lines. The research was conducted at a screenhouse of Muara Research Station, Indonesia Center of Rice Research (ICRR) from November to December 2017. Fourteen doubled haploid lines and 4 check varieties, namely Inpago 10 and Limboto, Salumpikit (drought tolerant check) and IR 20 (drought sensitive check) were used in this study. The experiment was arranged in a randomized complete block design with three replications. The observations were conducted on leaf rolling, leaf drought, and recovery ability. Based on Friedman nonparametric analysis, HR-5-7-1-1 and HR-7-15-2-1 had moderate response with estimated median values for leaf rolling of 3.44 and 3.00, respectively. Three doubled haploid lines namely HR-2-27-2-7, HR-2-34-1-3 and HR-7-15-2-1 had moderate response to drought with estimated median value of leaf drought of 3.56. Nine lines of doubled haploid had tolerant response with estimated median value of recovery ability of 1.00-1.56. Based on weighted selection index, nine doubled haploid lines with positive and high selection index were identified; however, only two lines (HR-7-15-2-1 and B3-2) had selection index higher than Inpago 10 and Limboto.Keywords: drought tolerance, leaf rolling, recovery, weighted selection index

Penentuan Indeks Seleksi untuk Galur Dihaploid Padi Sawah Tadah Hujan Berdaya Hasil Tinggi Akbar, Miftahur Rizqi; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Sugiyanta, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Rainfed rice breeding for high yield is an alternative to increase national rice production. The breeding can beaccelerated using anther culture technique. The selection of high productivity lines may be more effective if it also involvesthe traits contributing to or affecting the yield traits. This study aimed at determining suitable characters as selection criteriaand obtain a selection index model for high yielding doubled haploid rainfed rice lines. The experiment was conductedin a greenhouse of ICABOGRAD, Bogor and Sawah Baru Experimental Station, Bogor Agricultural University using arandomized complete block design (RCBD) with three replications. Thirty doubled haploid lines derived from anther cultureand two check varieties namely Ciherang and Inpari 18 were used. The results showed that plant height (TT), number of filledgrain per panicle (GI), and productivity (PRD) could be used as suitable selection characters. The determination of selectionindex obtained a selection model which was Selection index = 0.48 PRD + 0.31 JGI - 0.31 TT. This model can be used toselect high yielding doubled haploid rainfed rice lines.

Seleksi Galur-galur Dihaploid Padi Hitam pada Uji Daya Hasil Lanjutan Kuncoro, Danu; Sani, Mutiyara; Purwoko, Bambang Sapta Purwoko; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Maharijaya, Awang; Widajati, Eny; Bambang Sapta Purwoko
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 3 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Padi hitam merupakan sumber karbohidrat yang bermanfaat untuk kesehatan. Pengujian daya hasil merupakan tahapan penting dalam menentukan keberhasilan pemuliaan padi beras hitam. Tujuan penelitian ini ialah untuk memperoleh informasi keragaan agronomi dan menyeleksi galur-galur dihaploid padi beras hitam yang memiliki penampilan agronomi baik dan berdaya hasil tinggi untuk pengujian multi lokasi. Penelitian ini dilakukan dari bulan November 2019 sampai Juli 2020 di Bogor, Jawa Barat dan Malang, Jawa Timur. Sebanyak 23 galur dihaploid padi hitam yang memiliki sifat agronomi baik dengan produktivitas tinggi dan 3 varietas pembanding yaitu Aek Sibundong, Jeliteng, dan Inpari 24 digunakan sebagai materi genetik. Studi ini menggunakan rancangan rancangan kelompok lengkap teracak dengan 3 ulangan pada setiap percobaan. Hasil penelitian menunjukkan bahwa interaksi genotipe dan lingkungan berpengaruh nyata terhadap semua karakter pengamatan kecuali pada karakter lama pengisian gabah berdasarkan analisis keragaman. Sebanyak 14 galur yang terseleksi berdasarkan indeks terboboti memiliki karakteristik jumlah anakan produktif per rumpun dari 15.8 hingga 25.9, umur panen genjah dari 115.2 to 121.9 hari setelah semai, dan produktivitas >5.4 ton ha-1. Keragaan agronomi dan daya hasil galur-galur dihaploid padi yang terseleksi pada penelitian ini memerlukan pengujian lebih lanjut melalui uji multi lokasi untuk memperoleh calon varietas unggul baru padi beras hitam. Kata kunci: agronomi baik, indeks seleksi, kriteria seleksi, kultur antera padi

Co-Authors ,, Mawaddah AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Awang Maharijaya Bakhtiar, Bakhtiar. Bambang Sapta Purwoko Chaerani ,, Chaerani Cucu Gunarsih da Cunha, Rojino Danu Kuncoro Desta Wirnas Eko Sulistyono Endang Gati Lestari Eny Widajati Heni Safitri Iskandar Lubis Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartika Ning Tyas Muhamad Syukur Munandar, Arief Nindita, Anggi Nita Kartina, Nita Nurul Khumaida Oteng Haridjaja Purbokurniawan . Purwoko, Bambang Sapta Purwoko Purwoko, Bambang Sapto ROSSA YUNITA Safitri, dan Heni Sani, Mutiyara Sintho Wahyuning Ardie Siti Nurhidayah Slamet Susanto Sugiyanta, dan Trikoesoemaningtyas Willy Bayuardi Suwarno Wira Hadianto, Wira

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