Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Annales Bogorienses

Improvement of Genetic Transformation Efficiency inĀ Vanda tricolor OrchidĀ Using Acetosyringone Dwiyani, Rindang; Purwantoro, Azis; Indrianto, Ari; Semiarti, Endang
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for improving Vanda orchid through genetic modification could be valuable for horticulture and, indirectly, also for conservation. In this research, a method of Agrobacterium-mediated transformation of two V. tricolor obtained from Salak Mount, West Java and Merapi Mount, Yogyakarta in Indonesia protocorms was improved using acetosyringone (AS). Concentrations of 0 and 25 ppm AS were used in transformation of pG35S binary vector containing kanamycin resistance geneinto V. tricolor protocorms. The result showed that 25 ppm AS was required on inoculation with Agrobacterium solution, without AS on cocultivation. Five weeks after treatment on the 300 ppm kanamicyn containing medium, green protocorms were obtained, that was 11.01% for V. tricolor from Salak Mount with pre-culture treatment prior to inoculation, 9.39% for V. tricolor from Merapi Mount with pre-culture treatment prior to inoculation, and 1.37% for V. tricolor from Merapi Mount without pre-culture treatment prior to inoculation. The best condition to set high efficiency of transformation is pre-culture protocorms prior inoculation, soaking protocorm on 25 ppm AS for 30 minutes, then co-cultivate its on AS-free callus induction medium.
Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Bimantara, Putu Oki Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO ENDANG SEMIARTI Endang Semiarti Endang Semiarti ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti Ida Ayu Putri Darmawati IDA AYU PUTRI DARMAWATI Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Pradana, I Kadek Wira Purba, Reza Stefany Purwantoro, A - Putu Suwardike Redana, I Made Basma Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani