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Edison Edison
Bagian Kesehatan Masyarakat Fakultas Kedokteran Universitas Andalas
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Journal : Teknologi dan Industri Pertanian Indonesia

 1 
Komposisi dan Identifikasi Senyawa Metabolit Sekunder pada Rumput Laut Merah (Gelidium sp) dengan Metode Maserasi Salma, Putri; Edison, Edison; Diharmi, Andarini
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 17, No 1 (2025): Vol. (17) No. 1, April 2025
Publisher : Agriculture Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/jtipi.v17i1.34796

Abstract

Seaweed is one of the main aquaculture commodities that has economic value and has anormous potential in Indonesian. However the use of seaweed, especially the Gelidium sp type, is still undertutilitized. This research aims to determine the chemical composition and secondary metabolite compounds contained in the red seaweed Gelidium sp. The method used was experimental with different solvents, namely metanol, ethyl acetate and n-hexane in the extraction process. The test parameter analysis carried out was proximate analysis and analysis of secondary metabolite compounds in the red seaweed Gelidium sp. The results of the research were that in the proximate analysis, the water content was 14.42% in the wet weight, the ash content was 8.94% in the dry weight, The fat content was 1.25% in the drg weight and the carbohydrate content was 58.12%. The high carbohydrate content in seaweed is related to the presence of crude fiber components contained. Analysis of secondary metabolite compounds showed the presence of alkaloids, steroids, saponins and phenolics in the methanol solvent; alkaloid and steroid content in ethyl acetate; and alkaloids and steroids in n-hexane solvent. Secondary metabolite compounds in red seaweed Gelidium sp. It can be used as an antioxidant, antibacterial or medicine in the health sector
Aktivitas Antioksidan dan Inhibitor Alfa Glukosidase dari Fraksi Berbeda Rumput Laut Cokelat (Sargassum sp.) Diharmi, Andarini; Manurung, Rahel Eriani Maria; Edison, Edison
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 17, No 1 (2025): Vol. (17) No. 1, April 2025
Publisher : Agriculture Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/jtipi.v17i1.33228

Abstract

Sargassum sp. is one of the brown seaweed that contains bioactive compounds, which have antioxidant and alpha glucosidase inhibitor activity. This research aimed to determine the rendemen, secondary metabolic, total phenolic, antioxidant, and alpha glucosidase inhibitor from the extract and fractions of Sargassum sp. The research method used was an experiment by extracting Sargassum sp with methanol and the extract were fractionated with different polarity solvents (n-hexane, ethyl acetate, and ethanol). Data were analyzed descriptively. The analysis parameters consisted of rendemen, secondary metabolic (qualitative), total phenolics, antioxidants (DPPH and FRAP), and alpha glucosidase inhibitors. The results showed that the rendemen of the n-hexane, ethyl acetate, and ethanol fractions were 4,15, 5,81, and 4,94%, respectively. The secondary metabolic analysis of methanol extract were alkaloids, steroids/terpenoids, saponins, and phenolic. The n-hexane fraction were steroids/terpenoids, saponins, and phenolic. The ethyl acetate fraction were alkaloids, steroids/terpenoids, saponins, and phenolic. The ethanol and water fractions contained only saponins. The total phenolic content of the methanol extract, n-hexane, ethyl acetate, and etanol fractions were 21161,03, 7854,46, 1626,89, and 80,17 ppm. Antioxidants in methanol extract, n-hexane, ethyl acetate, ethanol fractions using DPPH were 329,42, 466,25, 535,08, and 80,17 ppm. Antioxidants in methanol extract, n-hexane, ethyl acetate, and ethanol fractions using FRAP were 196,15, 294,88, 364,26, and 492,68 ppm. Alpha glucosidase inhibitor in n-hexane fraction with an IC50 value of 538110,9 ppm. Extract and fractions of Sargassum sp. with different polarity solvents contained different secondary metabolic. Saponins were found in all solvents, phenolics and steroids/terpenoids were found in methanol extract, n-hexane, and ethyl acetate fractions, alkaloids were found in methanol exract and ethyl acetate fraction. The n-hexane fraction showed the highest activity in total phenol, antioxidant, and alpha glucosidase inhibitor.
Co-Authors A Rahman Abdiana Abdiana, Abdiana Abdullah, Assyari Abu Anwar Agus Hendrayady Ahmad, Latifah putri Alfiandri Alfiandri Alghifary, Zaki Andari Mukhtasida, Bunga Andarini Diharmi Andrian, Salsabila Syifa Andrian, Teddy Andriani, Andarini Angelia, Inge Ardhiyan Saputra, Ardhiyan Arie Putra Usman Astuti, Dwi Sutri Ayu, Sukma Bin Anuar, Arman Chandra Herawan Dalas, Ici Damanik, Apriliani Maranatha Dasril, Oktariyani David Bastian Sihombing David H.M. Hasibuan Dede Martino Delwita, Delwita Denny Denmar Desy, Hidayati Dewita Dewita Diana Pebriani Daulay Dompak Napitupulu, Dompak Elbina Mamla Saidah, Elbina Mamla Elfianty , Lena Elwamendri Emy Kernalis, Emy Eri Suhartanto Fadhlami, Putri Insani Fahmi, Ainul Faiza Rosa, Audrey Fdw, Dhiky Ferawati, . Fernando, Henry Fitri Kurnianingsih Fuad Muchlis Gawise, Gawise Gustanela, Oktrina Handayani, Sri Hanuna Shafariah, Hanuna Hasrinal, Hasrinal Hendra Setiawan Hendri Hendri Hermaya, Anggi Angelita Hesti Hesti Hidayati Desy Hitami, Munzir Husniah, Wa Ode Iriyadi Iriyadi Irtania, Irtania Jayus Jayus Juliandari, Yayangsi Karnila, Rahman Karnila KHAIRI, AKMAL Kurniawan, Unhaluddin T. Lavlinesia, lavlinesia M. Bisri Malindo, Robi Mamla Saidah, Elbina Manan, Manan Manurung, Rahel Eriani Maria Maria Ulfa Marisa Elsera Mery Sukmiwati Mirna Ilza Moch. Nurdin Mochammad Imron Awalludin Muanas Muanas Muhammad Andriansyah, Muhammad Mutasir Mutasir, Mutasir N. Ira Sari Napitupulu, Diana Ria Winanti Novita Sary, Annisa Nurdini, Saras Ayu Adistya Nurhalimah, Andi Nurihsan, Achmad Juntika Nurwahyuni Oktariyani Dasril Paradise, Nadia Pelsi Sulaini, Pelsi Pery Rehendra Sucipta Phangestu, Steffany Jessica Rahim, Amilda Rahmaniar Rahmaniar, Rahmaniar rahmatiqa, chamy Ramadhani, Nuzul Ria Ratnawati Siata, Ratnawati Resta, Harinal Afri Rini Lestari Rio Mattajang, Rio Riri Oktari Ulma Robiyatun, Hayuna Rosiana, Elisa Rovilah, Kanza Rozaina Ningsih, Rozaina Rr. Ani Wijayati, Rr. Ani Sadikin, Sadikin Safaria, Ria Safitri, Dian Prima Salina, Salina Salma, Putri Samsaifil Samsaifil Samsaifil, Samsaifil Santi, Irnita Rosaria Sarjun, Sarjun Sary, Annisa Novita Seprianti, Nopita Sholihah, Aulia Putri Sihombing, David Bastian Simbolon, Sarwoedy Sirait, Afrinson Sitanggang, Demak Riwardy Situmeang, Ampuan Sri Handayani Sri Nurchaini, Dewi Sukendi Sukendi Sumaiyah, Sumaiyah Sumarto Sumarto Supriyadi, Jepri Suwestian, Firdaus Syahril, Suci Syahrul Syahrul Syamsu Yusuf, Syamsu T, Rosmawati Tan, David tari, Yurina Tarno Tarno Tri Samnuzulsari Tri Wahyu Saputra Triana, Wiwin Trisnadewi, Eliza Udi Pramiudi Ulqadri, Ulqadri Untung Sapta Veby Senopati Silam Wahbi, Moh Wati, Isna Wayu Eko Yudiatmaja Wijaya, Fatma Pandu Wijaya, Rasman Sastra Wiya Elsa Fitri Wulandari, Noni Yasril Yazid Yulianda, Sri Yuniar Yuniar Yupianti , Yupianti Yusi Riksa Yustiana, Yusi Riksa Yusma Damayanti Zuriana, Ema