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Journal : Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)

Determination of Aedes Spp. Mosquito Breeding Spots as a High-Risk Area Through Maya Index Analysis in Way Halim Bandar Lampung Athiyya Nurfadhilah; Emantis Rosa; M. Kanedi; Tugiyono Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.36

Abstract

Dengue Haemorrhagic Fever (DHF) is a disease that is transmitted through the Aedes aegypti and Aedes albopictus mosquito vectors. The Maya index (MI) is an indicator to identify whether an area is at high risk as a breeding site for mosquitoes. This study aims to determine the types of breeding places and types of mosquito larvae of DHF and MI vectors in the Way Halim District of Bandar Lampung. The method used was a survey method on 100 houses in 5 villages in Way Halim District, namely Jaga Baya 1, Jaga Baya 2, Jaga Baya 3, Way Halim Permai, and Gunung Sulah. Data analysis to determine MI uses the Breeding Risk Index (BRI) and Hygiene Risk Indicator (HRI) indicators. The results of this study indicate that there were 225 containers inside the house observed, where 51 containers of them were found to have Aedes mosquito larvae while outside the house found as many as 28 containers with 8 containers including larvae. The identification results found 2 types of Aedes mosquitoes namely, Ae. aegypti (88.24%) and Ae. albopictus (11.76%). The results of the MI analysis show that Way Halim District is classified as a District with a moderate MI category with an MI value of 46%
Proteolytic Activity of The Entomopathogenic Fungi (Penicillium sp. ) of Cockroaches (Periplaneta americana) Ahmad Ikhsanudin; Emantis Rosa; Christina Nugroho Ekowati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.47

Abstract

Cockroaches (Periplaneta americana) were the insect vectors of disease that caused adverse effects on human health. Control cockroch excessive use of insecticides can lead to residus in the environment and resistance cockroach. Therefore it was necessary to use an alternatives such as such as entomopathogenic fungal as biologycal agents. The entomopathogenic fungi penetrated via the integument of an cockroach to reach the hemocoel. Proteins were the molecules responsible for integument strength in cockroach, It was synthesis the proteases to degrading proteins. The study begins with the isolation of entomopathogenic fungi using the moist chamber method with cockroach as insect bait. Fungus that grow on cockroches are cultured and purifed on Potato Dextrose Agar (PDA) medium then identified. Identification was carried out through macroscopic observations including colony color and diameter and microscopic observations including conidia, conidiophores, hyphae, vesicles, fialids, and leg cells. The result of isolation and identification obtained as Penicillium sp. Proteases enzimatic activity tested on PDA with anlene 1%. The clear zone formed is measured to show the activity of proteases produced by Penicillium sp.
Isolation and Identification of Fungi from Bactrocera dorsalis as Candidate Entomopathogenic Fungi Damayanti, Rizka Dwi; Emantis Rosa; Setiawan, Wawan Abdullah; Handayani, Tundjung Tripeni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.359

Abstract

Cultivation of horticultural crops especially fruits, has long been practiced in Indonesia. Citrus plants are one of the main fruit commodities developed in Indonesia. Increased citrus production is inseparable from challenges, especially in controlling harmful pest attacks. Bactrocera fruit fly has attracted worldwide attention because of its ability to attack more than a hundred horticultural crops and cause crop failure. Various ways have been done to control Bactrocera, such as protecting the fruit with wrapping and even the use of synthetic insecticides that cause many losses. To overcome this, innovations are needed using biological control. Entomopathogenic fungi are potential candidates for bioinsecticides. This study aims to isolate and identify entomopathogenic fungi from Bactrocera dorsalis from citrus plants. The entomopathogenic fungus isolation method uses the moist chamber method. Then the fungal isolates will be identified macroscopically and microscopically. The isolation results show that five types of fungi are identified from the body of Bactrocera dorsalis. The five fungi can be used as candidates for entomopathogenic fungi.