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All Journal JOURNAL TEKNIK SIPIL DAN INFRASTRUKTUR REKA GEOMATIKA Cannarium (Jurnal Ilmu-ilmu Pertanian) JURNAL PENELITIAN SAINTIKA Jurnal Medik Veteriner Jurnal Akta Yudisia Jurnal Kacapuri : Jurnal Keilmuan Teknik Sipil Journal of Pharmaceutical and Health Research JOURNAL LA MEDIHEALTICO Interaksi Peradaban: Jurnal Komunikasi dan Penyiaran Islam Prosperity: Journal of Society and Empowerment JIFI (Jurnal Ilmiah Farmasi Imelda) Al-MIKRAJ: Jurnal studi Islam dan Humaniora International Journal of Public Health Excellence (IJPHE) Indonesian Research Journal on Education Jurnal Kebidanan, Keperawatan dan Kesehatan (J-BIKES) Jurnal Kedokteran Hewan Parapemikir Jurnal Ilmiah Farmasi Jurnal Pengabdian Masyarakat Ilmu Kesehatan Lisanul Arab: Journal of Arabic Learning and Teaching Annales Bogorienses
Muhammad Gunawan
Pusat Penelitian Bioteknologi Lembaga Ilmu Pengetahuan Indonesia, Bogor
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Journal : Jurnal Kedokteran Hewan

 1 
INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Madihah, Madihah; Nafisah, Ghina
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10896

Abstract

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.1412.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 1000.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.
PERKEMBANGAN EMBRIO SAPI SETELAH FERTILISASI MENGGUNAKAN METODE INTRACYTOPLASMIC SPERM INJECTION (ICSI) DAN AKTIVASI DENGAN STRONTIUM Gunawan, Muhammad; Fahrudin, Mokhamad; Boediono, Arief
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2651

Abstract

Penelitian ini bertujuan mengetahui pengaruh kombinasi intracytoplasmic sperm injection (ICSI) dan aktivasi dengan strontium untuk mengetahui perkembangan pronukleus dan perkembangan embrio sampai tahap blastosis. Kombinasi ICSI dan strontium 20 mM meningkatkan perkembangan pronukleus 2-PN mencapai 43,59%. Hasil perkembangan embrio pada perlakuan kombinasi ICSI dan strontium 20 mM mencapai tingkat perkembangan 2-4 sel (50,5%), 8-16 sel (43,73%), dan blastosis (15,63%). Kesimpulan dari hasil penelitian ini adalah kombinasi ICSI dengan aktivasi strontium 20 mM mampu menghasilkan perkembangan in vitro embrio sapi yang lebih baik.
Co-Authors - Indrianawati - Sumarno Ahmed, Tanjeel Albar Albar ana, safri Andilala Ani Nur Aeni, Ani Nur Anwar, Laila Arfan Abrar Arief Boediono Arifin B. Arum Setiawan Asep Firmansyah Az Zahra, Nur Auliyah Bagas F, Muhammad Bagas, Muhammad Candani, Mella Cantika, Puja Widya Dewi Santika Dewi, Risnawati Diana Andrianita Kusumaningrum, Diana Andrianita Dwi Putranti, Oktora Ekowati, Indah Agustina Fadillah, Rizka Fatimah, Cut Fitriani, Enny Harianti, Evi Hasanah, Fenny Ida Sri Oktaviana Kaiin, Ekayanti Mulyawati Kartiawati Alipin Kasmuri Kasmuri, Kasmuri Koswara, Eko Kusumastuti, Melati Yulia Lala, Andi Langgeng Priyanto, Langgeng Lestari, Anggun Lijea, Isma Ae Madihah Madihah, Madihah Mokhamad Fahrudin Nafis Azmi Amrullah Nafisah, Ghina Nasution, Annio Indah Lestari Ningsih, Afni Sari NURUL AZIZAH Oktora Dwi Putranti Panjaitan, Sariatmaja Pitoyo, Pitoyo PriyantoPriyanto, Langgeng Putri, Alya Rosandiyus Ramadani, Muhammad Riswandi Riswandi Robiah, Minchatur Rusherdiannita, Raden Cindy Safriana Safriana Safriana Sari, Secillia May Setianingsih, Eva Siregar, Rizki Marwiyah Susanda, Apriansyah Suyatno Suyatno Tanjung, Siti Aisyah Tatan Kostaman Triansyah, Rizky Ahmad Ulwiyah Wahdah Mufassirin Liana Yuli Arif Tribudi Zukhaira Zultinur Muttaqin