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Journal : Poltekita : Jurnal Ilmu Kesehatan

Screening for the Detection of IgG anti-Toxoplasma gondii Antibodies in Female Donors at Semarang Regency Afrianti, Dina; Putri, Adita Puspitasari Swastya; Wikandari, Ririh Jatmi; Wahyudi, Wahyudi
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.2862

Abstract

Toxoplasmosis is a widespread zoonotic disease caused by Toxoplasma gondii (T.gondii). Blood and blood products are possible routes of transmission of T. gondii, especially in patients who have undergone multiple transfusions. The aim of this study is to determine the seroprevalence of toxoplasmosis in female donors using blood serum which is analyzed serologically to detect ImmunoglobulinG (IgG) using the Enzyme-Linked Fluorescent Assay (ELFA) method, as well as the relationship between risk factors for toxoplasmosis, such as keeping cats, receiving blood transfusions, eating undercooked meat and eating raw vegetables. This research is an analytic observational study using a cross-sectional study design. The sample taken is female donor candidates who are willing to participate during the study period, from March to September 2023, and have met the inclusion criteria to become research subjects by explaining the purpose and procedure of the study and the consent letter signed by each research subject before blood samples were taken and filling out the research questionnaire. 177 blood serum samples of female donors were collected from blood donor candidates using consecutive sampling method and a questionnaire to obtain risk factor data that influence the incidence of toxoplasmosis. The results showed that the seroprevalence of toxoplasmosis in the blood of female donor at Indonesian Red Cross Blood Donor Unit (UDD PMI) Semarang Regency, Central Java Province was 26.6%. In this study the risk factors that influence the incidence of toxoplasmosis are consuming raw vegetables with 5.835 times more risk than women who do not consume raw vegetables, while other factors such as keeping cats, receiving blood transfusions, and eating undercooked meat, have no significant effect. The seroprevalence of toxoplasmosis in female donors at UDD PMI of Semarang Regency is quite high due to the habit of eating raw vegetables or fresh vegetables with a very high transmission potential.
Evaluation of Toxoplasmosis in Female College Students Wikandari, Ririh Jatmi; Afriansya, Roni; Kuncara, Rachmad Bayu; Afrianti, Dina; Setyowatiningsih, Lilik
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.3209

Abstract

Female students are a group of women of childbearing age who can become pregnant and have risk factors for toxoplasmosis which can cause pregnancy disorders, birth defects, and death. Seroepidemiological studies of toxoplasmosis in women are essential to determine whether infection occurs in the early stages of fertilization or earlier. The research aimed to determine the incidence of toxoplasmosis in female college students. This research was carried out in June 2023 and has received ethical recommendations from KEPK Poltekkes Kemenkes Semarang. The type of research used was analytical descriptive. Sampling used purposive sampling. The inclusion criteria were 87 students who were willing to have their blood taken. Blood is taken using the venipuncture method, then the whole blood is separated from the plasma. Plasma samples were collected and analyzed for the presence of anti-Toxoplasma gondii using the ELISA method. Then the data was analyzed using SPSS software, univariate analysis to determine the frequency distribution and percentage of each variable. The results showed that 31 students were positive for IgG anti-Toxoplasma gondii. Of these, the majority aged 20 years (n = 20, 16.1%) were positive for anti-Toxoplasma gondii IgG. Toxoplasmosis infection of 9.2% occurred in female college students who owned cats, 3.4% occurred in female college students who consumed undercooked meat, and 29.8% of female college students who consumed raw vegetables. Female college students have risk factors for Toxoplasma gondii infection so it is important to carry out screening so that Toxoplasma gondii can be detected and treated immediately.