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Journal : Berkala Ilmiah Pertanian

PERUBAHAN KANDUNGAN ANTIOKSIDAN, POLIFENOL DAN PROFIL PROTEIN SELAMA PRA-PERKECAMBAHA PADA BIJI KAKAO Ulfaniah, Kiki; Handoyo, Tri; Sakdiyah, Zahratus
Berkala Ilmiah Pertanian Vol 1, No 3: FEBRUARI
Publisher : Berkala Ilmiah Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.406 KB)

Abstract

[ENGLISH] Cocoa proven as food source that rich in bioactive compounds, especially polyphenols that have a role as an antioxidant because it can stop the free radical reactions. As the change in lifestyle of the people and the development of technology, various ways have been made to improve the nutrition quality of the cocoa beans with pre-germination methods. This research aimed to study the effect of pre-germination methods that change the content of antioxidants, polyphenols and protein profiles of cocoa beans. The research was conducted in the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, University of Jember, held from January 28 until April 30, 2013. Metodology research used Sulawesi cocoa beans type 1 beans without skin and with the seed coat, which is done with a completely randomized design (CRD) in the old pre-germination 0, 1, 2, 3, 4, 5 days and repeated 4 times. The results showed that pre-germination methods provide a very real effect on the polyphenol content and antioxidant cocoa beans without without and the seed coat. The highest antioxidant content of cocoa beans without the skin of 0.47 µg/mg at the old pre-germination for 3 days and cocoa beans with the seed coat by 0.41 µg/mg in the old pre-germination 5 days. Polyphenol content of cocoa beans without skin and with the best seed coat that is in the control treatment had the highest content of 2.67 µg/mg for cocoa beans without skin and 2.46 µg/mg for cocoa beans with the seed coat. Keywords: Cocoa; Polyphenols; Antioxidants; Free Radicals; Pre-Germination [INDONESIAN] Kakao terbukti sebagai sumber makanan kaya senyawa bioaktif terutama polifenol yang mempunyai kasiat sebagai antioksidan karena mampu menghentikan reaksi radikal bebas. Seiring perubahan pola hidup masyarakat dan berkembangnya teknologi, berbagai cara telah dilakukan untuk meningkatkan kualitas nutrisi pada biji kakao dengan metode pra-perkecambahan. Penelitian ini bertujuan untuk mengetahui pengaruh metode pra-perkecambahan terhadap perubahan kandungan antioksidan, polifenol dan profil protein biji kakao. Penelitian dilaksanakan di Laboratorium Genetika dan Pemuliaan Tanaman, Fakultas Pertanian, Universitas Jember, dilaksanakan mulai tanggal 28 Januari sampai 30 April 2013. Metode penelitian menggunakan biji kakao jenis Sulawesi 1 tanpa kulit dan dengan kulit biji, yang dilakukan dengan Rancangan Acak Lengkap (RAL) pada lama pra-perkecambahan 0, 1, 2, 3, 4, 5 hari dan diulang sebanyak 4 kali. Hasil penelitian menunjukan bahwa metode pra-perkecambahan memberikan pengaruh sangat nyata terhadap kandungan polifenol dan antioksidan biji kakao tanpa kulit dan dengan kulit biji. Kandungan antioksidan tertinggi untuk biji kakao tanpa kulit biji sebesar 0,47 µg/mg pada lama pra-perkecambahan 3 hari dan untuk biji kakao dengan kulit biji sebesar 0,41 µg/mg pada lama pra-perkecambahan 5 hari. Kandungan polifenol biji kakao tanpa kulit dan dengan kulit biji terbaik yaitu pada perlakuan kontrol memiliki kandungan tertinggi sebesar 2,67 µg/mg untuk biji kakao tanpa kulit dan 2,46 µg/mg untuk biji kakao dengan kulit biji. Kata kunci: Kakao; Polifenol; Antioksidan; Radikal Bebas; Pra-Perkecambahan How to citate: Ulfaniah K, T Handoyo, Z Sakdiyah. 2014. Perubahan kandungan antioksidan, polifenol dan profil protein selama pra-perkecambaha pada biji kakao. Berkala Ilmiah Pertanian 1(3): 43-46.
PERUBAHAN KANDUNGAN ANTIOKSIDAN, POLIFENOL DAN PROFIL PROTEIN SELAMA PRA-PERKECAMBAHA PADA BIJI KAKAO Kiki Ulfaniah; Tri Handoyo; Zahratus Sakdiyah
Berkala Ilmiah Pertanian Vol 1 No 3 (2014): FEBRUARI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.406 KB)

Abstract

[ENGLISH] Cocoa proven as food source that rich in bioactive compounds, especially polyphenols that have a role as an antioxidant because it can stop the free radical reactions. As the change in lifestyle of the people and the development of technology, various ways have been made to improve the nutrition quality of the cocoa beans with pre-germination methods. This research aimed to study the effect of pre-germination methods that change the content of antioxidants, polyphenols and protein profiles of cocoa beans. The research was conducted in the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, University of Jember, held from January 28 until April 30, 2013. Metodology research used Sulawesi cocoa beans type 1 beans without skin and with the seed coat, which is done with a completely randomized design (CRD) in the old pre-germination 0, 1, 2, 3, 4, 5 days and repeated 4 times. The results showed that pre-germination methods provide a very real effect on the polyphenol content and antioxidant cocoa beans without without and the seed coat. The highest antioxidant content of cocoa beans without the skin of 0.47 µg/mg at the old pre-germination for 3 days and cocoa beans with the seed coat by 0.41 µg/mg in the old pre-germination 5 days. Polyphenol content of cocoa beans without skin and with the best seed coat that is in the control treatment had the highest content of 2.67 µg/mg for cocoa beans without skin and 2.46 µg/mg for cocoa beans with the seed coat. Keywords: Cocoa; Polyphenols; Antioxidants; Free Radicals; Pre-Germination [INDONESIAN] Kakao terbukti sebagai sumber makanan kaya senyawa bioaktif terutama polifenol yang mempunyai kasiat sebagai antioksidan karena mampu menghentikan reaksi radikal bebas. Seiring perubahan pola hidup masyarakat dan berkembangnya teknologi, berbagai cara telah dilakukan untuk meningkatkan kualitas nutrisi pada biji kakao dengan metode pra-perkecambahan. Penelitian ini bertujuan untuk mengetahui pengaruh metode pra-perkecambahan terhadap perubahan kandungan antioksidan, polifenol dan profil protein biji kakao. Penelitian dilaksanakan di Laboratorium Genetika dan Pemuliaan Tanaman, Fakultas Pertanian, Universitas Jember, dilaksanakan mulai tanggal 28 Januari sampai 30 April 2013. Metode penelitian menggunakan biji kakao jenis Sulawesi 1 tanpa kulit dan dengan kulit biji, yang dilakukan dengan Rancangan Acak Lengkap (RAL) pada lama pra-perkecambahan 0, 1, 2, 3, 4, 5 hari dan diulang sebanyak 4 kali. Hasil penelitian menunjukan bahwa metode pra-perkecambahan memberikan pengaruh sangat nyata terhadap kandungan polifenol dan antioksidan biji kakao tanpa kulit dan dengan kulit biji. Kandungan antioksidan tertinggi untuk biji kakao tanpa kulit biji sebesar 0,47 µg/mg pada lama pra-perkecambahan 3 hari dan untuk biji kakao dengan kulit biji sebesar 0,41 µg/mg pada lama pra-perkecambahan 5 hari. Kandungan polifenol biji kakao tanpa kulit dan dengan kulit biji terbaik yaitu pada perlakuan kontrol memiliki kandungan tertinggi sebesar 2,67 µg/mg untuk biji kakao tanpa kulit dan 2,46 µg/mg untuk biji kakao dengan kulit biji. Kata kunci: Kakao; Polifenol; Antioksidan; Radikal Bebas; Pra-Perkecambahan How to citate: Ulfaniah K, T Handoyo, Z Sakdiyah. 2014. Perubahan kandungan antioksidan, polifenol dan profil protein selama pra-perkecambaha pada biji kakao. Berkala Ilmiah Pertanian 1(3): 43-46.
IDENTIFIKASI KARAKTERISTIK MORFOLOGI DAN MOLEKULER 21 VARIETAS PADI AROMATIK (Oryza sativa L.) BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA) Irza Guari Syah Fitri; Tri Handoyo
Berkala Ilmiah Pertanian Vol 2 No 2 (2019): MEI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (804.3 KB) | DOI: 10.19184/bip.v2i2.16174

Abstract

ABSTRACT Aromatic rice (Oryza sativa L.) is one type of rice that has aroma and high selling price than non-aromatic rice. The variety development is needed to form superior aromatic rice. The variety development requires information about kinship and diversity between varieties. Kinship analysis with identification of morphological characteristics is an observation based on taxonomic approach. This research aims to determine the morphological characteristics and kinship of 21 aromatic rice varieties. The research was conducted from March to September 2018 with a randomized block design (RBD) of 3 replications. Observation variables used plant height (cm), number of productive tillers (tiller), age of flowering and harvesting (days), weight of 100 grain (gram), shape of grain, color of grain, color of rice and DNA band pattern as molecular data. Morphological data were analyzed by variance (ANOVA) and cluster analysis with UPGMA program (Unweight Pair Group Method with Arithmetic Mean) using DIST Coefficient on NTSYS 2.02 software. Molecular data showed in visualisation of DNA band using Primer OPB-07 based on RAPD marker. The research results showed the differences in morphological characters and kinship levels. Cluster analysis forms 2 clusters and 2 sub-clusters. Keywords: Aromatic Rice, Mophological Diversity, RAPD, Dendrogram ABSTRAK Padi aromatik (Oryza sativa L.) merupakan salah satu jenis padi yang memiliki aroma wangi dan memiliki harga jual yang lebih tinggi dibanding padi biasa. Pengembangan varietas diperlukan untuk membentuk padi aromatik yang lebih unggul. Pengembangan varietas membutuhkan informasi tentang kekerabatan dan keanekaragaman antar varietas. Analisis kekerabatan dengan identifikasi karakteristik morfologi merupakan suatu observasi berdasarkan pendekatan taksonomi. Percobaan ini bertujuan untuk mengetahui karakteristik morfologi dan molekuler 21 varietas padi aromatik. Percobaan dilakukan di mulai Maret - September 2018 dengan Rancangan Acak Kelompok (RAK) 3 ulangan. Variabel pengamatan menggunakan tinggi tanaman (cm), jumlah anakan produktif (batang), umur berbunga dan panen (HSS), bobot 100 gabah, bentuk gabah, warna gabah, dan warna beras serta pola pita DNA sebagai data molekuler. Data morfologi dianalisis dengan ragam (ANOVA) serta dianalisis gerombol dengan program UPGMA (Unweight Pair Group Method with Arithmetic Mean) menggunakan DIST Koefisien pada software NTSYS 2.02. Data molekuler ditunjukkan dalam bentuk visualisasi DNA menggunakan primer OPB-07 berdasarkan penanda RAPD. Hasil percobaan menunjukkan perbedaan karakter morfologi dan tingkat kekerabatan yang berbeda. Analisis gerombol membentuk 2 cluster dan 2 sub-cluster. Kata kunci: Padi Aromatik, Kekerabatan Morfologi, RAPD, Dendrogram
The Growth and Yield of The Radishes (Raphanus Sativus L.) to doses of nitrogen fertilizer and Potassium fertilizer Muhammad Syauqi; Tri Handoyo
Berkala Ilmiah Pertanian Vol 5 No 3 (2022): Agustus
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (574.272 KB) | DOI: 10.19184/bip.v5i3.15645

Abstract

The Radish (rhaphanus sativus l.) is a horticultural plant that enters into the bulbous bulbous variety. Radishproductivity has always decreased by 2.16%, although in 2016 its productivity increased by 0.11% but it was still lower inproduction (Agricultural Statistics Center, 2017). The low production of turnips is caused by highlanders and few problemsof fertilizing them. One attempt to improve the production of focused turnips on growing them at higher altitudes byexpanding areas where turnips are grown in lowland. A lowland area utilization effort to cultivate turnips by addingnitrogenous macro nutrients and potassium. Nitrogen plays a role as a chlorophyil compiler that is highly responsible for thephotosynthesis of plants and photosynthesizers secreted (Pahlevi, et. 2016). Potassium fertilizers themselves play a vital rolein sustaining turnips, durability of drought and disease pests. Aside from cultivating the use of varieties is something to takenote of in raising turnips. Superior varieties can provide the maximum yield by the growth of good plants. The experimentaims to identify the interaction of nitrogen and potassium fertilizer against the growth and horseradish yields (raphanussativus l.) The giving of the dose of nitrogen fertilizer used is N0 = 0 gr, N2 = 0.8 gr, N3 = 1 gr and potassium fertilizer doseis K0 = 0 gr, K1 = 0.2 gr, K2 = 0.4 gr, K3 = 0.6 gr. Data obtained subsequently was analyzed and tested using DuncanDMRT surplus 5%. Studies indicate that the best combination of treatment was that of a dose of n3 fertilizer treatment = 1 gr,and potassium k3 = 0,6 gr
Co-Authors Agus Sasmito, Agus Ahdatu Uli Khikamil Maulidiya Ahmad Ilham Tanzil Ahmad Rivaldy, Dhea Andari, Syamsiyah Yuli Dwi Arif, Muzayin Arifin, Muhammad Reza Arum, Ayu Puspita Assery, Syeh Azmi Saleh Azmia, Azmia Mar'atul Latipah Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kriswanto, Budi Candra Prayoga, Mohammad Damayanti, Dwi Putri Oktavia Dwi Putri Oktavia Damayanti Dwika Nano Hariyanto Efsari, Aqilla Nur Fanata, Wahyu Indra Duwi Fariza Oktaviani Galih Kusuma, Raditya Hakim, Mohammad Sulton Hakim, Saila Najma Haq, Ahmad Thoriq Izzul HARDIAN SUSILO ADDY Hopipah, Opi Intan Kartika Setyawati Irma Novita Sari Irza Guari Syah Fitri Juhardi, Ujang Kacung Hariyono Kartikasari, Subekah Nawa Khamidah, Khusna Kiki Ulfaniah Kiki Ulfaniah Laily Ilman Widuri Laily Ilman Widuri, Laily Ilman Latif, Arkanudin Lenny Widjayanthi Luh Putu Suciati Magfiroh, Illia Seldon Mahros Darsin Marcus dos Santos Soares MISWAR - Mochammad Amrun Hidayat Mohammad Ali Mudhor Mohammad Ubaidillah Muchammad Devara , Yusuf Muhammad Hazmi Muhammad Imron Rosadi Muhammad Nur Thoriq Alfy Muhammad Syauqi Munandar, Denna Eriani Nasti, Elyanisa Maharani Nisrina, Sofia Bunga Nur Widodo Parawita Dewanti Popy Hartatie Hardjo Pratiwi, Helti Anggiana Prayoga, Mohammad Candra Pudji Restanto, Didik Puji Rahayu Putu Gita Maya Widyaswari Mahayasih Rahadiyan, Aristyo Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Rizka Amalia Rony, Zahara Tussoleha Rudi Joelijanto Saputra, Tio Eka Saputro Gumelar, Bachtiar Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Slameto SRI AYU MADE Sri Hartatik Suryo Prakoso, Suryo Tri Agus Siswoyo Tri Wahyu Saputra Ubaidillah, Mohammad Umar Santoso Ummi Sholikhah Veronenci Yuliarbi Farlisa Wachju Subchan Wulanjari, Distiana Yani Corvianindya Rahayu Yugistia Bima, Rafi Yuli Witono Zahratus Sakdiyah Zahratus Sakdiyah Zaiyin Rizky Ageng Maulidia