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All Journal Biospecies Jurnal Lahan Suboptimal Biosfer: Jurnal Tadris Biologi Proceeding International Conference on Global Resource Conservation AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology Proceeding of the Electrical Engineering Computer Science and Informatics Science and Technology Indonesia Jurnal Penelitian Sains DIDAKTIKA BIOLOGI: Jurnal Penelitian Pendidikan Biologi IJEMS (Indonesian Journal of Environmental Management and Sustainability) Riau Journal of Empowerment Edumaspul: Jurnal Pendidikan Seminar Nasional Lahan Suboptimal Jurnal Gema Ngabdi Jurnal Vokasi Journal of Environmental Science and Sustainable Development Berkala Penelitian Hayati Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Indonesian Journal of Applied Environmental Studies SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal Jurnal Pepadu JURNAL BIOLOGI PAPUA Dikdimas : Jurnal Pengabdian Kepada Masyarakat Bulletin of Chemical Reaction Engineering & Catalysis Medicosphere Aksi Kita: Jurnal Pengabdian Kepada Masyarakat
Laila Hanum
Biology Department, Faculty Of Mathematics And Natural Sciences, Sriwijaya University, Indralaya, 30662, Indonesia
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Journal : Indonesian Journal of Applied Environmental Studies

 1 
Genetic variation based on RAPD (Random Amplified Polymorphic DNA) markers in western tarsiers (Cephalopachus bancanus) from South Sumatra and Bangka Island Pratiwi, Putri Rizki; Muharni, Muharni; Setiawan, Arum; Hanum, Laila; Yustian, Indra
Indonesian Journal of Applied Environmental Studies Vol 5, No 2 (2024): Volume 5 Number 2 October 2024
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/injast.v5i2.11039

Abstract

The western tarsier (Cephalopachus bancanus) is a nocturnal primate classified as Vulnerable by the IUCN. Understanding the genetic variation of tarsier populations in South Sumatra and Bangka Island is crucial for developing effective conservation strategies to protect this unique species. The research aims to determine the genetic variations of tarsiers from South Sumatra and Bangka Island by using 10 primers of the Random Amplified Polymorphic DNA (RAPD) molecular marker, namely ILO 525, ILO 1212, ILO 1204, OPE 16, OPE 17, OPE 19, OPF 06, OPF 04, OPY 03 and OPY 13. The RAPD primers utilized in this study are universal and effectively detect genetic diversity at interspecies and intraspecies levels. These primers have demonstrated the ability to produce clear and distinct electrophoresis band patterns in primates and other mammals. A total of six tissue samples were collected from Bangka Island, specifically from Petaling Village, Mendo Barat, and two additional samples were obtained from South Sumatra, originating from Selangit, Musi Rawas, and Padang Bindu, Ogan Komering Ulu. The Bangka Island samples were derived from a single population inhabiting a secondary forest characterized by shrubs and old rubber plantations. In contrast, the South Sumatra samples were obtained from secondary forest environments. Eight of the ten RAPD primers successfully amplified 89 DNA fragments, exhibiting a high degree of polymorphism. The genetic distance analysis, based on Dice coefficient values ranging from 0.000 to 0.629, revealed varying levels of genetic divergence among the samples. The bootstrap analysis further demonstrated that the relationships among all Western Tarsier (Cephalopachus bancanus) samples had a confidence level exceeding 50%. The observed high polymorphism reflects substantial genetic variability among the samples. RAPD markers thus offer a valuable tool for studies focusing on the relationships within closely related populations.
Co-Authors Aditya K. Karim Aditya K. Karim Adriansyah, Fikri Affandi, Azhar Kholiq Affrita, Tia Maya Afnur Imsya Aldes Lesbani Ali Djamhuri Amin Ali Mubarok Amin Rejo Andika Puspita Dewi Artanti, Widyana Beta Arum Setiawan Arum Setiawan Arum Setiawan Arum Setiawan Aryani, Robeah Ayu Nursafitri Ayu Safitri Chatrin Yohana Simamora Dedek Kurniawan Deni Julius Destia Maharani Destri, Erin Dewi Lisandra Doni Setiawan Dwi Asmono Dwi Septiawati, Dwi Eddy Ibrahim Edward Saleh Eka Haryati Yuliany Elisa Nurnawati Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Elyani Ami Endro Sastro cahyono Enggar Patriono Ervina Indrayani Ervina Indrayani Erwin Nofyan Evi Yuliza Evi Yuliza Evi Yuliza, Evi Fahira, Aulia Fidella Oktariana Fikri Adriansyah Fikri Adriansyah Fikri Adriansyah Fitri Maya Puspita Fitri Maya Puspita Fitri Maya Puspita Fitri Maya Puspita Hary Widjajanti hasana, nurdiah Helena Valenta Br Kemit Hernanda, Eggyana Deri Hikayati I Gusti Wayan Murjana Yasa Indra Yustian Indrawati Indrawati Irmawan Farindra Irmeilyana Juswardi Juswardi, Juswardi Lestari, Astri Indah Lestari, Margareta Sri Maretasari, Giri Margareta Sri Lestari Margareta Sri Lestari, Margareta Sri Maya Puspita, Fitri Melisyah, Melisyah Melwita, Elda Mery Hasmeda Muharni Muharni Mustafa Kamal Nabila Aprianti Negara, Zaidan Panji Ngudiantoro . Nina Tanzerina Nita Aminasih Nita Aminasih Nur Arifah Nuriessa Aputri, Farah Nursela, Dewi Parwanti, Sri Permata, Ending Pratiwi, Putri Rizki Putra Bahtera Jaya Bangun Putra Bahtera Jaya Bangun Putra BJ Bangun, Putra BJ R. Rugayah Rahmat Pratama Rahmat Pratama Rahmat Pratama, Rahmat Ranti Sawitri Ratih Wijayanti, Ratih Reza Andeka Rina S. Kasiamdari Rina S. Kasiamdari Rina Sri Kasiamdari Rizka Aniza Robeah Aryani Rosmania, Rosmania Rugayah Rugayah Rupiah Rupiah Rupiah Rusdi, Muhammad Salsabeela S. Santosa Salni Santika, Baitha Santosa . Santosa Santosa Sarno Sarno Sarno Sarno Sarno Sarno Sarno Sarno Singgih Tri Wardana Singgih Tri Wardana Sisca Octarina Sisca Puspita Sepriliani Siti Indah Oktaviani Siti Indah Oktaviani Siti Suzlin Supadi Suheyanto Suheryanto Sulaksono, Gogoh Syafrina Lamin, Syafrina Tri Handayani Venty Warda Elmaida Rusdi Wendra, Fahmi Wijaya, Alfan Windusari, Yuanita Yolanda Fitria Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yulia Anisawati Yuliany, Eka Haryati Zahrial Effendi Zaidan P Negara Zulkifli Dahlan Zulkifli Dahlan Zulkifli Dahlan