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Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Irma Dewiyanti; Darmawi Darmawi; Zainal Abidin Muchlisin; Teuku Zahrial Helmi; Iko Imelda Arisa; Cut Nanda Defira; Fitriyani Fitriyani; Sawva Yura
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

Histopathology status of black tiger shrimp (Penaeus monodon) in the conventional system in Bireuen, Aceh Province Dedi Fazriansyah Putra; Ulya Khairumi; Iko Imelda Arisa; Elzein Mohammed Fahal
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

The purpose of this study was to identify the tiger shrimp (Penaeus monodon) histopatology status in traditional cultured ponds at Bireuen Regency, Aceh Province. The sampling was carried out by purposively random method from seven ponds and two shrimps were sampled from each pond. Several water quality parameters were checked like temperature, pH, salinity and ammonia. Result showed that black tiger shrimp (Penaeus monodon) which were cultivated in traditional ponds in the Bireuen Regency, Aceh Province were histopathologically changes. It was suspected the hepatopancreas changes were associated with viral infection like Hepatopancreatica Parvovirus (HPV) and White Feces Disease (WFD). Ammonia concentrations were found higher in some ponds like Alu Buya Village, Jangka Keutapang Village, Jangka Mesjid Village, Alu Kuta Village and Punjot Village. The management of regular feeding and water quality control is highly recommended to anticipate the viral potential attack in traditional shrimp pond farming at Jangka District, Bireuen Regency, Aceh Province.Keywords:Tiger shrimps Histopathology Water quality

In vivo test of Litopenaeus vannamei infected by Vibrio using Moringa oleifera leaf extract Iko Imelda Arisa; Cut Mutia; Sri Agustina; Nurfadillah Nurfadillah; Sofyatuddin Karina
Depik Vol 10, No 2 (2021): August 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

This study aimed to determine the optimal concentration of Moringa oleifera leaf extract as an antibacterial agent on Litopenaeus vannamei larvae that are infected by Vibrio sp.. It was conducted in 15 March - 15 April 2019 at PT. Global Gain Superior Seeds, Pantai Cermin, North Sumatera. The research used a completely randomized design (CRD) method with five treatments and four replications. The shrimps infected by Vibrio sp. were immersed into M. oleifera leaf extract in various concentration of (A) 0 ppm, (B) 400 ppm, (C) 600 ppm, (D) 800 ppm, and (E) 1000 ppm for ±15 minutes. The size of shrimp used was post-larvae 8 (length of 3.5-5 mm/ind). The best performance results of vannamei shrimps were obtained in treatment E (1000 ppm), namely length growth of 16.75 ± 1.16 cm, weight growth of 0.92 ± 0.89 g and survival rate of 91±7.87%.Keywords:Moringa oleiferaVibrio sp.Immersion

Histopathology status of black tiger shrimp (Penaeus monodon) in the conventional system in Bireuen, Aceh Province Dedi Fazriansyah Putra; Ulya Khairumi; Iko Imelda Arisa; Elzein Mohammed Fahal
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.1.18715

The purpose of this study was to identify the tiger shrimp (Penaeus monodon) histopatology status in traditional cultured ponds at Bireuen Regency, Aceh Province. The sampling was carried out by purposively random method from seven ponds and two shrimps were sampled from each pond. Several water quality parameters were checked like temperature, pH, salinity and ammonia. Result showed that black tiger shrimp (Penaeus monodon) which were cultivated in traditional ponds in the Bireuen Regency, Aceh Province were histopathologically changes. It was suspected the hepatopancreas changes were associated with viral infection like Hepatopancreatica Parvovirus (HPV) and White Feces Disease (WFD). Ammonia concentrations were found higher in some ponds like Alu Buya Village, Jangka Keutapang Village, Jangka Mesjid Village, Alu Kuta Village and Punjot Village. The management of regular feeding and water quality control is highly recommended to anticipate the viral potential attack in traditional shrimp pond farming at Jangka District, Bireuen Regency, Aceh Province.Keywords:Tiger shrimps Histopathology Water quality

In vivo test of Litopenaeus vannamei infected by Vibrio using Moringa oleifera leaf extract Iko Imelda Arisa; Cut Mutia; Sri Agustina; Nurfadillah Nurfadillah; Sofyatuddin Karina
Depik Vol 10, No 2 (2021): August 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.2.17510

This study aimed to determine the optimal concentration of Moringa oleifera leaf extract as an antibacterial agent on Litopenaeus vannamei larvae that are infected by Vibrio sp.. It was conducted in 15 March - 15 April 2019 at PT. Global Gain Superior Seeds, Pantai Cermin, North Sumatera. The research used a completely randomized design (CRD) method with five treatments and four replications. The shrimps infected by Vibrio sp. were immersed into M. oleifera leaf extract in various concentration of (A) 0 ppm, (B) 400 ppm, (C) 600 ppm, (D) 800 ppm, and (E) 1000 ppm for ±15 minutes. The size of shrimp used was post-larvae 8 (length of 3.5-5 mm/ind). The best performance results of vannamei shrimps were obtained in treatment E (1000 ppm), namely length growth of 16.75 ± 1.16 cm, weight growth of 0.92 ± 0.89 g and survival rate of 91±7.87%.Keywords:Moringa oleiferaVibrio sp.Immersion

Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Irma Dewiyanti; Darmawi Darmawi; Zainal Abidin Muchlisin; Teuku Zahrial Helmi; Iko Imelda Arisa; Cut Nanda Defira; Fitriyani Fitriyani; Sawva Yura
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.22964

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

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