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Journal : Jurnal Sain Veteriner

DISTRIBUSI SARAF PADA EPITEL OLFAKTORIUS KALONG KAPATUK (Pteropus vampyrus) DAN LASIWEN DEIGNAN (Myotis horsfieldii) BERDASARKAN EKSPRESI NEURON-SPECIFIC ENOLASE (NSE) Yosephine Nicolory Paula; Soehartini Jatman; . Ariana; Tri Wahyu Pangestiningsih; Teguh Budipitojo
Jurnal Sain Veteriner Vol 25, No 1 (2007): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5391.275 KB) | DOI: 10.22146/jsv.277

Abstract

Kelelawar merupakan salah satu keanekaragaman dan kekayaan satwa di Indonesia. Adanya keanekaragaman jenis makanan maupun habitat kelelawar menyebabkan terjadinya perbedaan dalam mencari dan cara makan dari berbagai jenis kelelawar. Keterkaitan antara struktur indra penciuman dengan jenis dan cara makan kelelawar sangat menarik untuk dieksplorasi, karena sampai saat ini sangat sedikit data mengenai hal tersebut. Penelitian ini menggunakan 3 ekor kalong betina dewasa dan 3 ekor lasiwen betina dewasa, sebagai sampel. Hidung sebagai sampel, kemudian dibuat preparat histologi dengan metode parafin dan  pewarnaan imunohistokimia terhadap ekspresi NSE. Hasil pewarnaan imunohistokimia menunjukkan bahwa imunoreaktivitas NSE pada lasiwen terindentifikasi dalam epitel olfaktorius di kedua sisi septum nasalis, konka dorsalis dan beberapa fila olfaktoria. Pada kalong tidak terindentifikasi adanya imunoreaktivitas NSE.Kata kunci: saraf, epitel olfaktorius, kalong, lasiwen, Neuron-Specific Enolase(NSE)
Kajian Imunohistokimia Perkembangan Bentuk Neuron Atekolaminergik Pada Area Postrema Monyet Ekor Panjang (Macaca fascicularis)= Morphological Development of Catecholaminergic Neurons in the Area Postrema of Long-tailed.. Tri Wahyu Pangestiningsih; Koeswinarning Sigit; Dondin Sajuthi; Nurhidayat .
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2980.906 KB) | DOI: 10.22146/jsv.354

Abstract

rea postrema merupakan sepasang penonjolan ke dorsal pada bagian kaudal medula oblongata yang berbatasan dengan ventrikel IV. Area ini berperan sebagai chemoreceptor trigger zone (CTZ) pada proses muntah yang juga melibatkan neuron katekolaminergik (neuron KA). Pada kemoterapi penderita kanker, fungsi AP sebagai CTZ diupayakan untuk ditekan agar tidal( ada refleks muntah pada pasien. Dalam rangka lebih memahami neuron KA tersebut, dalam penelitian ini dilakukan pengamatan perkembangan bentuk neuron KA di area postrema (AP) monyet ekor panjang (MEP) mulai fetus (F) umur 40 sampai anak (P) umur 105 hari secara imunohistokimia menggunakan antibodi terhadap enzim tirosin hidroksilase (TH). Hasil penelitian memperlihatkan neuron KA di medula oblongata belwn terlihat pada F40, dan bare dijumpai pada F55 di daerah bakal AP, bentuk bulat dengan inti besar dengan sitoplasma sedikit yang merupakan ciri perkembangan awal bentuk neuron. Prosesus sitoplasma yang pendek pada mulai ditemukan pada F85, dan neuron KA di AP berubah menjadi bipolar pada F100 yang merupakan tanda perkembangan menengah bentuk neuron. Dengan bertambahnya umur, prosesus sitoplasma neuron KA bipolar bertambah panjang yang merupakan ciri tingkat perkembangan bentuk akhir dan ditemukan dominan di AP pada P105. Dari hasil penelitian ini dapat disimpulkan bahwa neuron KA di AP pada MEP berada dalam stadium perkembangan bentuk awal dan menengah selama masa prenatal, dan stadium perkembangan lanjut menuju ke perkembangan akhir terjadi pada masa postnatal.
Kuantitas Dan Kualitas Sel Telur Anjing Lokal Dari Berbagai Stadium Estrus Yuda Heru Fibrianto; Tri Wahyu Pangestiningsih; Amelia Hanna; Pudji Astuti; ClaudeM Mona Airin; Asyhari .; Amrullah Anindito; Nuraini Rahmawati; Koko Kurniawan; Pradityo Yoga Wibowo
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1310.87 KB) | DOI: 10.22146/jsv.411

Abstract

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Perbandingan Pengaruh Penambahan Cairan Folikel Sapi dengan Babi terhadap Maturasi Oosit Anjing (Canis familiarsis) secara in Vitro dari Stadium Estrus Ovarium Yuda Heru Fibrianto; Tri Wahyu Pangestiningsih; Amelia Hana; Claude Mona Airin; Nuraini Rahmawati
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1847.065 KB) | DOI: 10.22146/jsv.427

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Pemasakan Oosit Anjing (Canis familiarsis pada Stadium Anestrus dan Diestrus pada Media Maturasi yang Diperkaya dengan Cairan Folikel Sapi 10% dengan Penambahan Follicle Stimulating Hormone (FSH) dan Luteotropic Hormone (LH) Yuda Heru Fibrianto; Amelia Hana; Tri Wahyu Pangestiningsih; Pradityo Yoga Wibowo; Claude Mona Airin
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4904.308 KB) | DOI: 10.22146/jsv.430

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Developmental Anatomy of Cerebellum of Long-Tailed Macaque (Macaca fascicularis) at the First Trimester of Gestation Tri Wahyu Pangestiningsih; Irene Linda Megawati Saputra; Agnya Sinung Suminar
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4578.798 KB) | DOI: 10.22146/jsv.5429

Abstract

Long tailed macaque was one of animal models in biomedical research because it has  many similarities with humans, both anatomical and physiological properties. There were many research about cerebellum associated with its role in the coordination of muscle activity. Understanding of normal development of cerebellum long tailed macaque may help to understand about the development in human cerebellum and its abnormalities. Embryonic and fetal brain samples were obtained through caesarean section and were  then made for histological preparation stained with cresyl violet. Staining results were observed using a microscope with a digital camera. Images obtained are processed by graphics software Adobe Photoshop CS 8.0. Cerebellum Macaca fascicularis Ed40 showed the isthmus and rhombic lip that were composed of ventricular layer, mantle layer, and marginal layer. Cerebellum Macaca fascicularis Fd55 showed future lobes and future  fissures, but the cortex and medulla are not bounded clear. The cortex consisted of the external granular layer, neuroblast basket, and neuroblast stellate, while the  medulla consisted of neuroblast deep cerebellar nuclei. From this research, we concluded that neurons were on stage of proliferation and migration in the embryo aged 40 days, then differentiated and migrated to form cortex  cerebellum and deep cerebellar nuclei at the age of 55 days, but the development of the cerebellum was not fully completed yet. 
A Study of the Fruit Bat (Rousettus sp) Brain Anatomy as Natural Reservoir Wild Animal for the Rabies Virus Karina Mayang Sari; Siti Khomariyah; Nur Arofah; Sabrina Wahyu Wardhani; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1507.935 KB) | DOI: 10.22146/jsv.6530

Abstract

Rousettus sp. (Fruit bat) is one type of fruit bats in Indonesia and act as a natural reservoir of rabies. Rabies is caused by a virus from genus Lyssavirus, family Rhabdoviridae, which attack central nervous system (CNS).The brain is an organ that is sensitive to rabies infection. The purpose of this study was to determine the anatomical structure of the fruit bat brain macroscopically. Five fruit bat were used in this study, they were anaesthetized using ketamine and xylazin. Animals were perfused using physiological saline and 10% buffered formalin. Brains were taken using tweezers after all the bones of the skull were separated. Analysis of macroscopic brain was done descriptively. The results showed that the fruit bat brain were generally divided into cerebrum, cerebellum and brain stem. Gyrus, sulcus and the paraflokulus lobes of the fruit bat brain were less developed than that of the dogs brain.
THE CHANGES OF THE TESTOSTERONE HORMONE LEVEL DURING PREPUBERTAL PERIODE IN INDUCED HYPOTHYROIDISM RATS Teguh Budipitojo; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8619

Abstract

The one the influence factors to the normal functions and testes growth is thyroid gland status. The knowledge about the influences of euthyroid, and hypenhyroid to thetesies growth will help to give more understanding of the normal lestes growth. The new born male rats were used todetect the influence of hypothyroidism to the testosterone level during prepubertal periode. Twenty primigravida pregnant rats (Rattus norvegicus albinus) were used. After birth, the male pups were divided inio controls and treatments group. Mothers of the treatment group were given 0,1% 6-n-prapyl-2-thiauracil (PTU), via drinking water, ad libitum, after birth until on day 8. On dary 27, 30, 37, 44, and 51, five control pups and 5 treatment pups were sacrificed. The blood from each pup were collected to detec serum testosteron hormone level using immunoradLometric assay (IRMA) method. The research showed that serum testosteron level ofthe; treated group increased on day 30 (P<0,05) and day 37(P<0,05). Theresultof This research showed that PTU induced hypothyroidism produced increasing serum testosterone level.
EFFECTS OF RIBOFLAV1N SUPPLEMENTATION ON THE TERATOGENIC EFFECTS OF SODIUM BORATE IN WHITE RATS Tri Wahyu Pangestiningsih; Sumiyati Sunaryo
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1149.871 KB) | DOI: 10.22146/jsv.9173

Abstract

Na-borate gave teratogenic effects both to rats and chickens. In chickens the fetal malformations caused by borate treatments were similar to those due to riboflavin deficiency. It was proposed that in embryonated eggs borate built a bond with riboflavin, so that riboflavin could not junction properly. This present investigation was conducted to assess the possibility of riboflavin  in  prevent  the  teratogenic effects of  Na-borate in rats.Nineteen primigravida Wistar rats were allocated randomly to one of treatment groups, i. e, 9 rats were orally treated with 180 mg/200g BW/day, No-borate the other 10 rats beside treated with Na-borate, they were also treated orally with 2.5 mg/rat/day riboflavin. The treatments were done during the pregnancy on day 6 to 15. On day 20 the pregnant does were caesarean sectioned under ether anaesthesia. After all fetal malformations were noted, then all fetuses were placed in alcohol 70%, prepared for alizarin method for microscopic skeleton inspection. Chi square analysis of the gross inspection malformations, i e. the resorbed vs. the unresorbed, the alive vs. the dead, the haemorrhage vs. the non Hemorrhage fetuses of the two treatments showed unsignificant difference. Riboflavin supplementation increased singnificantly the number of fetuses with omphalocel. T-test analysis indicated that riboflavinsupplementation highly significantly caused the fore and hind paw, costal, sternal defects became more serious, if also significantly reduced the weight and the length of the fetuses. This investigation revealed that riboflavin supplementation failed to prevent, but even caused the teratogenic effects of the Na-borate to rats became more serious.
Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani dengan Metode Imunohistokimia Avidin-Biotin-Peroxidase Complex Teguh Budipitojo; Ariana Ariana; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (881.22 KB) | DOI: 10.22146/jsv.31314

Abstract

Glucose Transporter 4 (GLUT 4) merupakan protein transporter glukosa yang diatur oleh insulin, ditemukan terutama di jaringan adiposa dan otot lurik (baik otot skelet maupun otot jantung). Ayam kedu cemani merupakan ayam endemik Indonesia yang terdapat di wilayah Kedu, Temanggung, Jawa Tengah. Penelitian ini diperlukan untuk melengkapi dokumentasi data anatomi mikroskopik kekayaan hayati asli Indonesia. Penelitian ini bertujuan untuk mengklarifikasi distribusi GLUT 4 dalam serabut otot skelet ayam kedu dengan metode imunohistokimia avidin-biotin-peroxidase complex (ABC). Penelitian dilakukan dengan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani. Hasil penelitian menunjukkan bahwa immunoreaktivitas GLUT 4 terutama terdeteksi di sarkolema dan komponen miofibril pada otot pectorales mayor, biceps brachii, maupun biceps femoris. Intensitas imunorekatifitasnya meningkat dari intensitas lemah pada otot pectorales mayor, menjadi intensitas sedang pada otot biceps brachii, dan kemudian intensitas kuat pada otot biceps femoris. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani lainnya, untuk mendokumentasi data anatomis kekayaan hayati asli Indonesia.
Co-Authors . Ariana . Muttaqien Agnya Sinung Suminar Agus Purnomo Amelia Hana Amelia Hanna Amrullah Anindito Anak Agung Gede Sugianthara Aqiilah, Khairunnisa Ariana Ariana - Ariana Ariana Ariana Ariana Ariana Ariana Arvendi Rachma Jadi Arvendi Rahma Jadi Asyhari . Ayu Miftahul Khasanah Bambang Dwi Raharjo Bambang Dwirahardjo Bambang Sugito Bambang Sugito Bambang Sutrisno Budi Susilo Budiariati, Vista Cahya Yustisia Hasan, Cahya Yustisia Citra Ayu Pramesti Claude Mona Airin ClaudeM Mona Airin Daisynta Prima Aninditya Dewi Kania Musana Dewi Kania Musana Diah Tri Widayati Dondin Sajuthi Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Erdiansyah Rahmi Erni Sulistiawati Erwin . Erwin E Etriwati E Filphin Adolfin Amalo Gerarda Gita Puspitandaru Heni Pujiastuti Hery Widijanto Hery Wijayanto Hevi Wihadmadyatami Hevi Wihadmadyatami Iffah Sofana Intan Maharani Irene Linda Megawati Saputra Irma Padeta Jaswadi J Jaswadi J Karina Mayang Sari Koeswinarning Sigit Koko Kurniawan Kustiati, Ulayatul Muttaqien M Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Niswah Nurul Fahma Nur Arofah Nuraini Rahmawati Nurhidayat . Nusaibah Nuraniyati Pradityo Yoga Wibowo PUDJI ASTUTI Rina Pratiwi Rini Widayanti Roza Azizah Primatika Sabrina Wahyu Wardhani Saragih, Golda Rani Sari, Yuslikha Khaniif Anggita Sitarina Widyarini Siti Khomariyah Soehartini Jatman Sumiyati Sunaryo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Trini Susmiati Victor Lenda Vidya Irawan Vidya Irawan, Vidya Woro Danur Wendo Woro Danur Wendo Yosephine Nicolory Paula Yuda Heru Fibrianto Yulfia N Selan Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik