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Takashi Reymond Rimpan
Universitas Pelita Harapan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Insertion of Cold Shock Gene From Escherichia Coli in Wheat Plants to Produce Drought Tolerant Properties Takashi Reymond Rimpan; Naomi Dwany; Geraldy John Meyners; Yohana Delvia Ayustin; Wahyu Irawati
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.03

Abstract

Drought is a threat that affects plant growth and development. Wheat crops are very sensitive to heat and drought stress during flowering and grain development, which negatively affects grain yield and quality. Wheat with drought tolerant properties is a genetically modified plant obtained by inserting a cold shock protein gene from Escherichia coli so that it can increase the ability of wheat to adapt to drought. This literature review aims to provide information about the role of cold shock protein gene insertion from Escherichia coli in wheat plants to produce drought tolerant traits. The results of the literature review showed that the CspA gene from Escherichia coli inserted into DNA could increase gene expression by changing the nucleotides in the DNA sequence to increase G+C. Gene insertion produces recombinant DNA with specific nucleotide changes. Nucleotide changes were carried out to increase the overall G+C amino acid content without changing the existing CspA and CspB protein sequences. These changes played an important role in helping wheat adapt to drought. The leaves on the transgenic wheat showed a decrease in the rate of water loss because the stomata in these plants closed faster than in the ordinary wheat so that the transgenic wheat became more drought tolerant.
Co-Authors Geraldy John Meyners Naomi Dwany WAHYU IRAWATI Yohana Delvia Ayustin