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Journal : Jurnal Fitopatologi Indonesia

Bakteri Endofit dari Tanaman Kehutanan sebagai Pemacu Pertumbuhan Tanaman Tomat dan Agens Pengendali Meloidogyne sp. Abdul Munif; Arif Rafi Wibowo; Elis Nina Herliyana
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.311 KB) | DOI: 10.14692/jfi.11.6.179

Abstract

Meloidogyne sp. is one of the main constraints of tomato production in Indonesia. Endophytic bacteria may be considered as biocontrol agents for controlling Meloidogyne sp. The objective of this study was to isolate endophytic bacteria from forestry plants and to evaluate its potential for controling Meloidogyne sp. on tomato. Endophytic bacteria were isolated from roots of mahoni (Swietenia mahogany), trambesi (Albizia saman), gaharu (Aquilaria malacensis), and meranti (Shorea sp.). Isolation of bacterial endophytes from plant tissue was conducted using surface sterilization method with 70% alcohol, 3% NaOCl and sterile water on medium trypsic soy agar. Endophytic bacteria was separated and purified based on shape and color of the colony. A total of 33 isolates of endophytic bacteria were isolated from roots of mahoni (11 isolates), trambesi (5 isolates), gaharu (7 isolates), and meranti (10 isolates). The bacteria was tested for the hypersensitivity reaction on tobacco plants and the result showed that 22 isolates did not cause necrosis, indicated they are not pathogenic. Ten isolates of endophytic bacteria was selected for further experiment, i.e. to evaluate their potential as biocontrol agent for Meloidogyne sp. and as growth promotor for tomato plants. The experiment was conducted in the screenhouse using seed treatment. The result showed that two isolates of endophytic bacteria, i.e. MSJ1H and AGS1F were able to increase the growth of tomato plants up to 60% and reduce the number of root knot caused by Meloidogyne sp. Endophytic bacteria isolated from forestry plants have the potential as a biocontrol agents to plant parasitic nematode Meloidogyne sp. 
Co-Authors Abdul Muhyi ABDUL MUNIF Abdul Munif ABDUL MUNIF Abdurachman Syafiih Achmad Achmad . Achmad . Achmad . Achmad ; Achmad Achmad ACHMAD ACHMAD Achmad Lisdar Adisti Permatasari Putri Hartoyo Agus Setiawan Ai Rosah Aisah Akhir, Jumadil ANANG PRANOTO HIDAYAT Ananta Kusuma Amanda Andi Sukendro Andrea Ajeng Eirenne Kristianti Anisa Tri Harjanti Ardiansyah Putra Ariana Ariana Arief B. Witarto Arief Noor Rachmadiyanto Arif Ravi Wibowo Arzyana Sunkar Asmoro, Prayogo Probo Benyamin Dendang Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Deasy Putri Permatasari Dewi Sukma Dodi Nandika Dwierra Evvyernie Dyah LINGGA NP Erianto Indra Putra Eti Artiningsih Octaviani Fatin Hanifah Felliks Tampinongkol, Felliks Fitri Kurniawati Fuzy Yustika Manik Gustan Pari Hanifah Nuryani Lioe Hayati Minarsih Hayati Minarsih Hidayati, Syafitri Hidayatullah, Deden Hutagalung, Labana I. Sudirman Iga Dwi Syahrani Illa Anggraeni Irfan Jelata, Tedi Irfan Kemal Putra Irma Badarina Iskandar Z Siregar Isroi Isroi Ivan Permana Putra Kulsum, Fifit Kultsum, Fifit Kunio Tsunoda Laila Fithri Maryam Libranika Dwi Miswati Liza Sakbani Lufthi Rusniarsyah, Lufthi Lul Godi, Rizal Lutfi Hanafi Melly Br Bangun Mira Febrianti Muhammad Alam Firmansyah Nabawiah, Safira Nifa Hanifa Noor Rachmadiyanto, Arief Nurulhaq, Muhammad Iqbal OSICA ASNO FERINA YURTI Ratna Jamilah Reny Meisetyani Reza Pamunca, Airres Rezeka Amalia Rizki Nugraheni Amaliasuci Rossy Nurhasanah Safira Nabawiah Santiyo Wibowo Sarah Diana Yulianti Shodiq Syifaudin, Ikhwan Silviana Arsyad Soetrisno Hadi Sri Listiyowati Sri Wahyuni, Devi Sri Wilarso Budi Surono Suryo Wiyono Tiara Antika Tjahja Muhandri Toto Toharmat TW DARMONO DARMONO Wartaka Wartaka ; Wasrin Syafii Yurico Bakhri Yusuf Sudo Hadi