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Hilda Hilda
Fakultas Hukum Universitas Tadulako, Palu
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Journal : Jurnal Kartika Kimia

 1 
Pengaruh Variasi Pelarut Etanol-Air Terhadap Aktivitas Antioksidan Ekstrak Tempe Karlina, Yenni; Sukrasno, Sukrasno; Riyanti, Soraya; Hairunnisa, Hairunnisa; Hilda, Hilda; Marselyun, Marselyun
Jurnal Kartika Kimia Vol 7 No 2 (2024): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v7i2.865

Abstract

Indonesia has a variety of processed food products made from beans, one of which is tempeh. A traditional Indonesian dish, tempeh is made from soybeans (Glycine max). The bioactive isoflavones contained in tempeh possess phenolic groups that contribute to its antioxidant properties. In addition to isoflavones, compounds such as superoxide dismutase and tocopherol also exhibit antioxidant activity. This study aimed to evaluate the antioxidant activity of tempeh extracts by varying the concentrations of ethanol in the solvent: 96% ethanol, 70% ethanol, 50% ethanol, 30% ethanol, and water. Extraction was performed using ultrasonic-assisted extraction, and antioxidant activity was assessed using the DPPH method, both qualitatively (via dynamolysis and TLC) and quantitatively (using UV-Visible spectrophotometry). The results from the qualitative antioxidant tests using dynamolysis and TLC showed potential antioxidant activity, evidenced by a yellow color change after the sample was sprayed with 0.2% DPPH reagent. Quantitative UV-Visible spectrophotometric testing with quercetin as a standard yielded an IC50 value of 2.86 µg/mL. The IC50results for the extracts in decreasing order of ethanol concentration were as follows: 96% ethanol extract (2638.49 µg/mL), 70% ethanol extract (2559.49 µg/mL), 50% ethanol extract (2288.65 µg/mL), 30% ethanol extract (1945.54 µg/mL), and water extract (2946.45 µg/mL). The highest antioxidant activity was observed in the 30% ethanol tempeh extract, while the lowest antioxidant activity was found in the water extract. These findings indicate that tempeh extracts exhibit antioxidant activity, which varies depending on the ethanol-water solvent composition.
Co-Authors Achmad, Andi Nurul Isnawidiawinarti Adrianto, Ratno Alfindo, Yuri Putri Almuntaha, Eska Aniah, Suci Nur Aprija Rosianti Ariefah Putri, Rosalin Arifin Hidayat, Arifin Arimbi, Siti Nurhaliza Arsyawina Arsyawina Aryanti Aryanti Aryanti ASEP SUPARMAN Asriyani, Asriyani Azzahra, Mia Bakhtiar, Rahmat Bambang Sudaryono Barlian, Barlian Cholik, M. Agus Dedi Rianto Dewi, Annisa Mellyana Dya, Dya Endah Wahyutri Fahrezi, M. Vilza Raihan Fajriah, Yustika Nur Ferawaty, Ferawaty Fidyah Faramita Utami Fifian, Fifian Gagaramusu, Yusdin Mahmudin Ghandy, Ghandy Hairunnisa Hairunnisa Hamdan, Hendro Hemalia, Hemalia Hesti Prawita Widiastuti Hoyauna, Hoyauna Idris, Nur Alfia Irfansyah Baharuddin Pakki Lazuardi, Luthfan Lestiani, Lilik Liyani, Unmul Marlindawati Marlindawati, Marlindawati Marselyun, Marselyun Masniah Masniah Maulana Abdul Aziz, Fikri Mifta Farid, M Mochammad Imron Awalludin Muis, Andi. Abd Musdariansyah, Musdariansyah Nabila, Amanda Salsa Nasirin, Nasirin Norsehan, Norsehan Nugraha, Irsyad Nulhakim, Lukman Nuraini Nuraini Nurjamin, Lucky Rahayu Nursakti Nursakti NURUL HIDAYAH Nurusyifa Azahra, Devalinda Pahriadi, Pahriadi Pamungkas, Leny Parellangi, Parellangi Pietersz, Jemmy Jefry Rachmania, Rachmania Ramadhani, Fira rasyid, hilda Ratnawati Ratnawati Richard Jokhu, Jean Rosalin Ariefah Putri Rr Sri Saraswati RR. Ella Evrita Hestiandari Ruri Octari Dinata Salmah Salmah Satriyo Nugroho, Iwan Sedubun, Victor Juzuf Sefrianus, Hendy Selvi Marcellia Setiadi, Rizky Shoufiah, Rahmawati Sitepu, Nadroh Soraya Riyanti, Soraya Sri Mulyati Sukensi, Sukensi Sukrasno Sukrasno Sulbadana Sulbadana Supriadi Supriadi Syalwa, Fitria Syamsiar Zahrani TATI ROHAYATI Tri Utami Lestari Ulina, Kartika Wakhid Sobirin, Muhammad Wijonarko, Wijonarko Yenni Karlina, Yenni Yudistira, Aji Yudha Yulianita Rahayu, Yulianita yusnita, henny Zuhdi, Naufal Zuhriatusobah, Juju Zulnuraini