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All Journal Health Science Journal of Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Skolastik Keperawatan (e-Journal) Jurnal Dunia Kesmas Multidiciplinary Output Research for Actual and International Issue (Morfai Journal) Indonesian Journal of Pharmacology and Therapy
Indwiani Astuti
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Journal : Indonesian Journal of Pharmacology and Therapy

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Cytotoxicity of hantap (Sterculia oblongata Mast) leaves extracts against breast cancer cell line (T47D): effect on apoptosis and caspase-3 mRNA expression Adika Suwarman; Indwiani Astuti; Woro Rukmi Pratiwi; Sitti Ayu Suhartina Yahya
Indonesian Journal of Pharmacology and Therapy Vol 5 No 2 (2024)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.10147

Abstract

Hantap (Sterculia oblongata Mast) leaves has been traditionally used in Palu, Central Sulawesi to treat breast cancer. However, its scientific evidence is limited. This study aimed to investigate the cytotoxicity of hantap leaf extracts against T47D breast cancer cells. The apoptotic activity of the extracts and its effect on caspase expression were also evaluated. The extracts were prepared by multilevel maceration using n-hexane, methanol, and water. Cytotoxic activity was evaluated by MTT assay. The apoptotic activity was observed by using a fluorescence microscope after acridine orange-propidium iodide (AO/PI) staining, whereas the caspase-3 mRNA expression was examined by using RT-PCR. Among the 3 tested extracts, the methanol extract exhibited the highest cytotoxicity with an IC50 value of 85 μg/mL. The methanol extract at concentrations of 42.5 μg/mL (½IC50), 85 μg/mL (IC50), and 170 μg/mL (2IC50) induced 127.25, 85.50, 479.5% of cell apoptosis, respectively. Furthermore, the methanol extract at concentrations of ½IC50, IC50, and 2IC50 increased 1.04, 1.43 and 1.69 time higher of caspase-3 mRNA expression. In conclusion, the methanolic extract of hantap leaf exhibits cytotoxicity against T47D breast cancer cells, by inducing apoptosis and increasing caspase-3 mRNA expression.
Co-Authors . Harijadi Adi Heru Sutomo Adika Suwarman Anis Widyasari Anwar, Sufyan Danarto Danarto Didik Setyo Heriyanto Ernawati Eva Flourentina Kusumawardani Hermawan, Dessy Husin, Hasanuddin Itza Mulyani MARNIATI Mohammad Juffrie Nur Arfian Putri, Enda Silvia Rachma Greta Putri Rina Susilowati Roy David Sarumpaet Sari Eka Pratiwi Sitti Ayu Suhartina Yahya Sofia Mubarika Haryana Sri Kadarsih Suci Eka Putri Sunarti Sunarti Suprihati . Woro Rukmi Pratiwi Yudanti Riastiti Zainal Arifin Nang Agus