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Journal : Journal of Veterinary and Animal Sciences

Identification of Growth Hormone Gene of Bali Cattle with Qualitative Superior in Bali Province Ni Ketut Suwiti; I Wayan Suardana; Ni Luh Watiniasih; I Nengah Kerta Besung; Chandra Yowani
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p09

Abstract

A research has conducted, in order to identify the genetic markers of Bali cattle that can be used as an indicator of their qualitative superior. The genetic marker identified was the growth hormone gen. As many as 50 whole blood samples which were collected from 50 individual cattle which were certificated as good breed or had qualitative superior, were used in this study. The DNA fragments were identified by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with primers, GH5 (5’-CCC ACG GGC AAG AAT GAG GC-3’ and GH6 (5’TGA GGA ACT GCA GGG GCC CA-3), respectively. Furthermore, the PCR products were restricted with MspI endonucleic restriction enzyme, before electrophoresis in 2% agarose The result showed that the growth hormone gene of bali cattle with qualitative superior has polymorphism which characterized by the formation of two fragments i.e. 230 and 329 bp that categorized as: allele +/+ and allele -/-.
Balinese and Landrace Pig Body Size Used for Spit Roast Pork Richard Christian Daud; I Wayan Suardana; I Putu Sampurna
Journal of Veterinary and Animal Sciences Vol 4 No 2 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i02.p01

Abstract

Abstract. Spit roast pork or commonly called “be guling” is food from pigs of approximately 3-4-month-olds, varied between female or male pigs, filled with spices and vegetables such as cassava and grilled (rolled) to be cooked, marked by color transformation from skin color to brownish read and crispy skin. In Bali Island, Balinese and Landrace pigs are commonly used for cooking spit roast pork. The pig selection is determined by the market price and customer needs. A high market price and demand such as ceremonies lead people to select small-sized pigs; however, a low market price and demand (sold for consumption) lead people to select large-sized pigs. This study aimed to examine Balinese and Landrace pig body sizes used for spit roast pork. The study was performed by measuring the pig's length, height, chest circumference, and abdomen circumference. Data collected were then analyzed using the T-test to discover the mean difference. Meanwhile, the variation difference was tested using the F-test. The study demonstrated that Balinese pig’s body size was insignificantly (P>0,05) smaller than the Landrace pig for spit roast pork on 3-4-month-olds. Balinese pig’s body size had a significantly larger variation (P<0.05) (more varied) than the Landrace pig on 3-4-month-olds. Keywords: Body size; Balinese pig; Landrace pig; Spit roast pork
Antimicrobial Susceptibility Patterns And DNA Plasmid Profiles Of Escherichia Coli O157:H7 Isolated From Feces Of Chicken I Wayan Suardana; Iwan Harjono Utama; Michael Haryadi Wibowo
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p01

Abstract

Escherichia coli O157:H7 has been identified as one of the most devastating microorganism causing diseases in human. The infection by this bacteria causes a wide spectrum of clinical manifestations ranging from asymptomatic, diarrhea or bloody diarrhea, up to serious clinical conditions such as hemorrhagic colitis, and hemolytic uremic syndrome. Poultry is known as one of its reservoir besides cattle and pig. Moreover, the use of antibiotic in animal feed as growth promoters is common worldwide. Due to its potential threat to public health, sensitivity test against various antibiotics and studying the plasmid DNA profiles are necessary. The study was initiated by cultivation of isolates, followed by test of antimicrobial susceptibility against various antibiotics and then by analyzing the plasmid DNA profiles. Results of study showed that among of 7 E. coli O157:H7 local isolates originated from chicken feces, as many as 42.9%; 14.3%; 14.3%; and 14.3% were resistant to 2; 3; 4; and 5 various of antibiotics, respectively. Base on the type of antibiotic, 85.7%; 71.4%; and 42.9% showed resistance to Methicillin, Penicillin G, Doxycycline and Streptomycin. Plasmid DNA of each isolates showed characters variation too, ranging from 2 to 4 bands with molecular weight ranging from 19.457 bp; 15.137 bp, 5.749 bp, 4.118 bp, 3.500 bp, 2.957 bp and 2.024 bp. Results of study indicated that local isolates of E. coli O157:H7 isolated from chicken showed multiple antibiotic resistance against various antibiotics, as well as its plasmid DNA profiles.
Molecular Analysis of Lactic Acid Bacteria SR4 Strain Isolated from Gastric’s Juice of Bali Cattle Iwan Harjono Utama; I Wayan Suardana
Journal of Veterinary and Animal Sciences Vol 4 No 2 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i02.p03

Abstract

Lactic acid bacteria SR4 strain is known to have potential as a probiotic source according to the previous study. The isolate derived from the gastric’s juice of bali cattle has characteristics such as its resistance to growth at low pH, namely pH 2, 3, and 4, its tolerance to bile concentration of 0.6 mM, and its inhibition against Gram-positive and negative bacteria. On the other hand, a complete molecular analysis of the isolate has never been carried out. Based on these considerations, the molecular analysis of the LAB SR4 strain becomes interesting to present. The study was initiated by cultivating LAB SR4 isolate on an MRS broth medium. The isolate was further confirmed molecularly through analysis of the 16S rRNA gene which was initiated by isolating genomic DNA followed by PCR reaction using universal primers 27F and 1492R. The PCR product of the 16S rRNA gene was sequenced and further analyzed using the MEGA 5.2 software. The data were then analyzed descriptively and presented in the form of tables or figures. The results showed that the LAB strain SR4 was identified as a Pediococcus pentosaceus. This strain potentially to develops as a source of bacteriocin and it can be applied as a bio preservative, improvement of the meat taste, inhibition of inflammation, and also as antagonism of cancer.
Complete Blood Profile and Neutrophil-Lymphocyte Ratio of Electrical Stunning and Non-Stunning Swine Bq Nurlita Anugrah; I Wayan Suardana; Hamong Suharsono
Journal of Veterinary and Animal Sciences Vol 5 No 2 (2022)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2022.v05.i02.p04

Abstract

Examination of the blood profile is crucial because blood functioned as an important role in the circulatory system and can be used to evaluate the emergence of stress in animals. The purposes of this research are to examine the complete blood profile and examine the possibility of stress symptoms by measuring the ratio of neutrophils-lymphocytes as the indicator of stress in electrical stunned and non-stunned swine. There were 40 six-month life swine with a weight of 80-100kg. The swine grouped into stunning and non-stunning group. A total of 3 ml of blood sample occupied and placed to a contained anticoagulant Ethylene Diamine Tetraacetic Acid (EDTA) vacutainer. The total of erythrocytes, hemoglobin, PCV, MCV, MCH, leukocytes and differential leukocytes were calculated using routine hematological examination methods with autoanalyzer machines. Based on the results of the research, the complete blood profile of electrical stunning slaughtered swine was healthier and more reliable than non-stunning. The ratio of neutrophils-lymphocytes in electrical stunning slaughtered swine was lower than non-stunning. This condition indicated a lesser amount of stress on the use of electric stunning.
Co-Authors Abdul Manan Achjar, Komang Ayu Henny Adirinata, I Komang Pasek Agus Sri Lestari Amrulloh, Muhammad Faqih Anak Agung Gde Bagus Udayana Andi Isma Lestari Amin, Andi Isma Ardika, I Gusti Ngurah Putu Ari Yuniastuti Aribaten, Ni Nengah Zinnia Arimbawa, I Gede Artayani, Ida Ayu Gede Asyauqi Ilham Perdana, Asyauqi Ilham Bambang Sumiarto Bq Nurlita Anugrah BUDI SETIADI DARYONO Budi Setiadi Dayono Chandra Yowani D. A. Widiasih Denny Widaya Lukman Devi Dwi Jayanti Dewa Gede Agung Widyadnyana Dewa Putu Oka Prasiasa Dewi, Ni Putu Diah Trisna Dewintasari, Ni Nyoman Paramitha Dwi Lestari Dyah Ayu Widiasih Dyah Ayu Widiasih Dyah Ayu Widiasih Eka Putri Suryantari Emmanuella Felice’anna Dije Karisoh Febrianti, Andri Nurdiana Franciska, Juliana Gama, I Ketut Gama, Ketut Gede Arimbawa, I Made Hana Kristal Alamanda Septiara Harini, I Gusti Ayu Hartati, Ni Nyoman Henny Achjar, Komang Ayu I Dewa Made Sukrama I G. Wijana I Gede Gian Saputra, I Gede I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Putu Bagus Sasrawan Mananda I Ketut Adi Sugita I Ketut Mangku Budiasa I Ketut Muka I Ketut Suada I Ketut Suardana I Ketut Suatha I Komang Gede Wiryawan I Made Adikampana, I Made I Made Kardena I Made Sukada I Made Sukarja I Made Walesa Putra I Nengah Kerta Besung I Nengah Sujaya I Nengah Wirakesuma, I Nengah I Nyoman Ariana I Nyoman Suarsana I Nyoman Sudiarta I Nyoman Sukma Arida I Nyoman, suardina I Putu Sampurna I Putu Sudiarta I Wayan Adnyana I Wayan Mustika I Wayan Seriyoga Parta I. H. U Utama Ida Bagus Ngurah Swacita Iga Prassetyo Adji, Iga Prassetyo INDAYATI LANYA Iwan Harjono Utama Karuni, Ni Kadek Khamid Yusuf Baehaqi, Khamid Yusuf Komang Januartha Putra Pinatih Kondra, I Wayan Korbinianus Feribertus Rinca Kumalasari, Ni Putu Putri Laba, I Nyoman MAS DJOKO RUDYANTO Meitisrilatifatulain Fitriadewi Mariana Mertanadi, I Made Michael Haryadi Wibowo Mita Ekamelinda Ngurah, I Gusti Ketut Gede Ngurah, IGK Gede Ni Kadek Lyming Lestari Ni Ketut Suwiti Ni Luh Sustiawati Ni Luh Watiniasih Ni Made Ariani Ni Made Ayu Aryati Dinarini Ni Made Ruastiti Ni Putu Ratna Sari Nuria Fitrianti Putri Nyoman Dewi Pebryani NYOMAN SEMADI ANTARA Oktivia Chandra Mustika, Oktivia Chandra P. Sampurna Padmi, Luh Sri Anggayoni Julia Piartrini, Saroyeni Pitriyantini, Putu Eka Pramesti, Kadek Diah Pratiwi, Ida Ayu Windhari Kusuma Putra, I Kadek Aldi Margareta Perdana Putu Ayu Sisyawati Putriningsih Putu Cahaya Semesta Putu Januari Ratna Apsari Putri R Susanti Raharjo, Anis Rasdianah Rasdianah Remawa, Anak Agung Gede Rai Reny Navtalia Sinlae Rian Ka Praja Ribek, Nyoman Richard Christian Daud Ruspawan, Dewa Made Ruta, I Made Satriawati, Ni Nyoman Ayu Siti Helmyati Sri Anggreni Lindawati Sri Wahyuni Suarya Putra, I Nyoman Agus Sudiantara, I Ketut Sudiantara, Ketut Suharsono, Hamong Sukoco, Hendro Sunatha Putra, Agus Surya, I Kadek Adi Tjokorda Sari Nindhia Totton, Mary Louise Victor Bangun Mulia Wahyu Hananto, Wahyu Wayan Tunas Artama Wayan Tunas Artama Widya Asmara Wilantari, Ni Nyoman Ayu Wulandari, Andi Dewi Wulandari, Kadek Dina Yan Ramona Yuli Darmawan, Yuli Yunita Sri Hastuti, Yunita Sri