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All Journal Jurnal Penelitian Saintek JBIO: jurnal biosains (the journal of biosciences) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Sciscitatio : Journal for Biological Science Life Science JURNAL BIOLOGI PAPUA

Nur Khikmah

Akademi Analis Kesehatan Manggala Yogyakarta

Author-ID : 2905812

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Articles

Title

EXAMINE ANTIBACTERIA ACTIVITY OF COMMERCIAL FERMENTED DAIRY PRODUCTS AGAINST PATHOGENS BACTERIA Nur Khikmah
Jurnal Penelitian Saintek Vol 20, No 1: April 2015
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The aim of this research was to examine antibacteria activity of commercial fermented dairy products against pathogens bacteria. The antibacteria activity was determined using the well diffusion method. The presence of antibacteria activity were shown by clear zone which formed around well containing fermented dairy products. The results show that yakult and probiotics yoghurt able to inhibit the growth of pathogens bacteria. The most sensitive pathogen bacteria to fermented dairy products was Salmonella thypii. Inhibition yakult and probiotics yoghurt against gram negative bacteria were greater than gram positive bacteria. Yakult containing Lactobacillus casei strain shirota was able to inhibit all pathogens bacteria, such as Salmonella thypii, Escherichia coli, Bacillus cereus, and Staphylococcus aureus.

UJI ANTIBAKTERI MASKER KEFIR SUSU KAMBING PADA Staphylococcus epidermidis SECARA IN VITRO Ajeng Desti Ningsih; Nur Khikmah
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk mengetahui antibakteri masker kefir susu kambing pada Staphylococcus epidermidis dan menghitung jumlah koloni Bakteri Asam Laktat (BAL) dan khamir. Uji antibakteri dilakukan dengan metode difusi sumuran. Antibakteri masker kefir ditunjukkan dengan adanya zona hambat yang terbentuk di sekitar sumuran. Spread plating dilakukan untuk menghitung koloni bakteri asam laktat dan khamir. Medium MRSA dan PDA diinkubasi pada suhu 37ºC selama 2-3 x 24 jam. Jumlah koloni yang tumbuh dihitung menggunakan metode Standard Plate Count dengan jumlah koloni 30-300, dan dinyatakan dalam satuan CFU/g. Hasil penelitian menunjukkan bahwa masker kefir mempunyai sifat bakteriostatik. Kemampuan antibakteri masker kefir susu kambing pada Staphylococcus epidermidis disebabkan karena di dalam supernatan masker kefir terdapat senyawa antibakteri. Hal ini ditandai dengan terbentuknya zona irradikal. Rerata koloni BAL pada masker kefir susu kambing A dan B adalah 1,5×109 dan 1,2×1010 CFU/g. Rerata jumlah koloni khamir pada masker kefir susu kambing A dan B adalah 2,1×1010 dan 3,0×1010 (3,9×1010) CFU/g.ANTIBACTERIAL OF GOAT’S MILK KEFIR MASK ON Staphylococcus epidermidis IN VITROThis study was aimed at determining the antibacterial goat’s milk kefir mask on Staphylococcus epidermidis and the number of Lactic Acid Bacteria (BAL) and the number of yeast colonies. The antibacterial test was carried out using the diffusion method of the wells. The antibacterial activity in the kefir mask is shown by the presence of inhibitory zones that form around the well. Spread plating was done to calculate the colonies of lactic and yeast acid bacteria. MRSA and PDA medium were incubated at 37ºC for 2-3 x 24 hours. The number of growing colonies is calculated using the Standard Plate Count method with the number of colonies of 30-300 and expressed in units of CFU/g. The results showed that kefir masks had bacteriostatic properties. The antibacterial ability of goat’s milk kefir mask was since the kefir supernatant contained antibacterial compounds. This is indicated by the formation of an nonradical zone. The mean of BAL colonies in goat milk masks A and B was 1.5 × 109 and 1.2 × 1010 CFU/g. The average number of yeast colonies in Goat milk masks A and B was 2.1 × 1010 and 3.0 × 1010 (3.9 × 1010) CFU/g.

POTENSI INFUSA DAUN NANGKA SEBAGAI OBAT KUMUR HERBAL Christ Alfianus Tosubu; Nunung Sulistyani; Nur Khikmah
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.38032

Penelitian ini dilakukan untuk mengkaji potensi daun nangka (Artocarpus heterophyllus) sebagai obat kumur herbal dengan melihat jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Daun nangka berwarna hijau muda yang diperoleh dari Babadan, Banguntapan, Bantul dibuat simplisia. Penentuan potensi dilakukan dengan menentukan perbedaan jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Uji potensi infusa daun nangka sebagai obat kumur dilakukan dengan menghitung perbedaan jumlah koloni bakteri rongga yang diperoleh dengan melakukan swab pada pangkal lidah sebelum dan sesudah berkumur. Perhitungan koloni bakteri dilakukan menggunakan metode hitung cawan (total plate count) pada media plate count agar (PCA). Hasil penelitian menunjukkan rata-rata jumlah koloni bakteri sebelum dan sesudah berkumur dengan infusa daun nangka yaitu 1,78 x 107 CFU/mL dan 7,71 x 106 CFU/mL. Perlakuan pemberian obat kumur infusa daun nangka secara signifikan mampu menurunkan jumlah koloni bakteri rongga mulut. Infusa daun nangka berpotensi sebagai alternatif obat kumur herbal. THE POTENCY OF JACKFRUIT LEAF INFUSION AS A HERBAL MOUTHWASHThis study was conducted to examine the potential of jackfruit (Artocarpus heterophyllus) leaves as a herbal mouthwash by identifying the growth of bacterial colonies in the oral cavity before and after gargling with jackfruit leaf infusion. The light green jackfruit leaves obtained from Babadan, Banguntapan, Bantul were made simplicia. The determination of potency was done by determining the difference in the number of bacterial colony growth in the oral cavity before and after gargling with jackfruit leaf infusion. The potential test of jackfruit leaf infusion as a mouthwash was carried out by calculating the difference in the number of cavity bacterial colonies obtained by swab at the base of the tongue before and after gargling. Bacterial colonies were counted using the total plate count method on plate count agar (PCA) media. The results showed that the average number of bacterial colonies before and after rinsing with jackfruit leaf infusion was 1.78 x 107 CFU/mL and 7.71 x 106 CFU/mL. The treatment of giving jackfruit leaf infusion mouthwash was significantly able to reduce the number of bacterial colonies in the oral cavity. Jackfruit leaf infusion has the potential as an alternative to herbal mouthwash.

THE RELATIONSHIP AMONG PEDICULOSIS CAPITIS, ANEMIA AND LEARNING ACHIEVEMENT IN ELEMENTARY STUDENTS Nunung Sulistyani; Nur Khikmah
Jurnal Penelitian Saintek Vol 24, No 2: Oktober 2019
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

This study was aimed at determining the relationship between the Pediculus humanus capitis infestation condition, anemia status and learning achievement in primary school-age children. This study was conducted at SD Bangunharjo Sewon Bantul, Yogyakarta. This study is an observational analytic study with cross sectional design in which the variables are measured in one measurement without intervening. The data obtained through pediculosis examination, Hb examination, and measurement of learning outcomes. The data then were analyzed descriptively presented in tabular form, then performed statistical analysis using the chi-square test. The results show that the students suffering from pediculosis, 57.9% also suffering from anemia and 51.0% having poor category achievement. In line with other researchers, one of the factors that influence one’s learning achievement is the memory factor. Someone’s anemia status can affect one’s memory. The prevalence of Pediculus humanus capitis infestation is 38.3%. Chi-square test showed a significant relationship between Pediculus humanus capitis infestation condition, anemia status, and learning achievement.HUBUNGAN PEDIKULOSIS KAPITIS, STATUS ANEMIA DAN PRESTASI BELAJAR PADA SISWA SEKOLAH DASAR Penelitian ini bertujuan untuk mengetahui hubungan kejadian infestasi Pediculus humanus capitis (kutu kepala) dengan status anemia dan prestasi belajar pada anak usia sekolah dasar. Penelitian ini dilakukan di SD wilayah Bangunharjo Sewon Bantul Yogyakarta. Penelitian ini merupakan penelitian analitik observasional dengan desain cross sectional. Variabel diukur dalam satu kali pengukuran dengan tidak melakukan intervensi. Data penelitian diperoleh melalui pemeriksaan pedikulosis, pemeriksaan Hb, dan pengukuran hasil belajar. Data dianalisa secara deskriptif yang disajikan dalam bentuk tabel, kemudian dilakukan analisis statistik menggunakan Uji chi-square. Hasil penelitian ini menunjukan bahwa menderita pedikulosis sebesar 57,9% menderita anemia dan sebesar 51,0% dengan prestasi belajar kategori kurang baik. Salah satu faktor yang mempengaruhi prestasi belajar seseorang yaitu faktor daya ingat. Status anemia seseorang dapat mempengaruhi daya ingat seseorang. Prevalensi infestasi Pediculus humanus capitis sebesar 38,3%. Uji chi-square menunjukkan adanya hubungan yang signifikan antara kejadian infestasi Pediculus humanus capitis dengan status anemia dan prestasi belajar.

Co-Authors Ajeng Desti Ningsih Barinta Widaryanti Christ Alfianus Tosubu Haloho, Apriani Nunung Sulistyani

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