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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) International Journal of Biosciences and Biotechnology Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES The Journal of Pure and Applied Chemistry Research Indonesian Journal of Chemistry Journal of Health Sciences and Medicine

Ketut Ratnayani

Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia

Author-ID : 2921952

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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KADAR TOTAL SENYAWA FENOLAT PADA MADU RANDU DAN MADU KELENGKENG SERTA UJI AKTIVITAS ANTIRADIKAL BEBAS DENGAN METODE DPPH (Difenilpikril Hidrazil) Ketut Ratnayani; A. A. I. A Mayun Laksmiwati; Ni P. Indah Septian P.
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Honey contains a variety of compounds which function as antioxidants one of which is a phenolic compound. Monoflora honey used in this study were randu and kelengkeng honey from certain brand distributed on the market. Qualitative test with 5% FeCl3 result showed that both types of honey contain phenolic compounds. The total phenolic compounds was determined by spectrophotometry using the Folin-Ciocalteu method and gallic acid as standards, while the free radical activity was tested using the method of DPPH (Difenilpikril hidrazil). The result of research showed that the total content of phenolic compound in randu was 1375,89 ± 134,10 mg GAE/kg, while kelengkeng honey was 1136,49 ± 39,62 mg GAE/kg. The % reduction of free radical in randu honey at the 5th minute was 62,55 ± 4,4407 % and at the 60th minute was 95,39 ± 8,5507 %. The % reduction of free radical for kelengkeng honey at the 5th minute was 44,12 ± 1,3433 %, 60th minute was 62,00 ± 0,8612 %, and for the standard of gallic acid the % reduction of free radical at the 5th minute was 41,03% and the 60th minute was 92,00%. Therefore, there was a linear correlation between the total phenolic compound of randu honey and kelengkeng honey with % reduction of its free radical.

AMPLIFIKASI FRAGMEN 0,4 KB DAERAH D-LOOP DNA MITOKONDRIA LIMA INDIVIDU SUKU BALI TANPA HUBUNGAN KEKERABATAN DENGAN METODE POLYMERASE CHAIN REACTION (PCR) K. Ratnayani; Sagun Chandra Yowani; Liangky Syane S
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 1 Januari 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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The human mitochondrial DNA (mtDNA) has higher polimorfism level than nucleous genom, and it ismaternally transmitted. D-Loop is a non-sense region in human mtDNA that has the highest polimorfism. Generally,the aim of this research is to find out the variation in D-Loop region of mtDNA in some Balinese without familycorrelation. For that reason, this research was brought out to determine the sequences of nucleotide of D-Loop regionin five normal Balinese from different families without correlation. The specific aim of this research is to amplify the0,4 kb fragment of mtDNA D-Loop region in five Balinese above, using the PCR methode. In conducting the PCR,the temperature of annealing of primer and the weight of template of mtDNA were optimized. Several phases thathave been conducted : 1). Lisis of the cavum oris epithelium; 2). Quantation of DNA; 3). Reaction PCR; 4). Result ofPCR detection with agarosa gel electroforesisThe result of this research is the amplification of 0,4 kb fragment of D-Loop region in mtDNA by PCR.This research also found the optimum temperature annealing, which was 55 0C, and the optimum weight of templateof DNA which was ± 0,688 ?g.

ANALISIS VARIASI NUKLEOTIDA DAERAH D-LOOP DNA MITOKONDRIA PADA SATU INDIVIDU SUKU BALI NORMAL Ketut Ratnayani; I Nengah Wirajana; A. A. I. A. M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Human mitochondrial DNA (mtDNA) has higher polimorfism level than nucleous genom, especially in theD-Loop region, which is a non coding region and the most polymorfic region in the mitochondrial genom. Theanalysis of variation of nucleotide sequence of D-Loop region can be used to determine the individual or ethnicidentity and also maternal familiar relationship. The research aims to determine nucleotide variant on Balineseindividue, which can be used as data base in determination of mtDNA genetical profile of Balinese ethnic in a biggerscale.To achieve the aims of the research, way the nucleotide sequence of one normal Balinese individue usingthe epithelia cells in the saliva. The methods were :1) the isolation of sample mtDNA, 2) the amplification of the DLoopregion of mtDNA by PCR, 3) sequencing and analysis of nucleotides sequence.The 0,4 kb fragment of the D-loop region mtDNA of the sample were successfully amplified, andsequenced of 402 pb. The research found 6 new variants or morfe different from Cambridge or Anderson sequence :variant 16223C®T, 16249T®C, 16259C®T, 16278C®T, 16316A®G, 16375C®A. The research also found thedeletion of T nucleotide on position 16362.

PENENTUAN LAJU REAKSI MAKSIMAL (Vmaks) DAN KONSTANTA MICHAELIS-MENTEN (KM) ENZIM LIPASE PANKREAS PADA SUBSTRAT MINYAK KELAPA, MINYAK SAWIT, DAN MINYAK ZAITUN Ketut Ratnayani; A. A. I. A. Mayun Laksmiwati; Maman Sudiarto
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

A main element in the Michaelis-Menten equation is Km, which is typical for a particular enzyme, with a specific substrate at a certain pH and temperature conditions. The aim of this study is determining the difference in the maximum rates (Vmax) and Michaelis-Menten constant (Km) of pancreatic lipase on the coconut oil, palm oil, and olive oil substrates and the most effective hydrolysis by the pancreatic lipases. Km value was calculated by measuring the rate of the catalyzed hydrolysis with various concentrations of pH, temperature, and the optimum incubation time. Before calculating the value of Vmax and Km, the initial rate (v0) was calculated with the titrimetric method. The results showed that Vmax was 2,11 × 10-3 mmol/min on coconut oil substrate; 2,30 × 10-3 mmol/min on palm oil substrates; and 1,60 × 10-3 mmol/minutes on olive oil substrate. While the pancreatic lipase Km values ??were 1,21 × 104 ppm on coconut oil; 2,29 × 104 ppm on palm oil; and 1,60 × 104 ppm on the olive oil. This results suggested the pancreatic lipase catalyzed the hydrolysis was most effective on coconut oil compared with palm oil and olive oil.

PENENTUAN KADAR GLUKOSA DAN FRUKTOSA PADA MADU RANDU DAN MADU KELENGKENG DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI K. Ratnayani; N. M. A. Dwi Adhi S.; I G. A. M. A. S. Gitadewi
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 2 Juli 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Honey is composed of reducing sugars i.e. glucose, fructose, and maltose. The concentration of sugar honeyis determined as total reducing sugars, so the concentration of each sugar which compose the honey is not known.The research aims to determine the concentrations of glucose and fructose of honey from different cotton tree honeyand longan honey HPLC using.The HPLC operational condition was as follows 80oC of column temperature and 1 mL/minutes of flowrate, using metacarb 87C column and deionized watr as eluent. The detection was carried out by using refractiveindex detector, where glucose and fructose can be separated at retention times of 6 and 7 minutes.The result of research showed that the concentration of glucose in cotton tree honey was 27.13 % and inlongan honey was 28.09 %. the concentration of fructose in cotton tree honey was 40.99 % and in longan honey was40.03 %. Thees results showed that the quality standard on the total concentration of reducing sugar (60 %) was metby both types of honey. The total concentration of reducing sugar of cotton tree honey was 68.12 % and of longanhoney was 68.12 %.

DESAIN PRIMER UNTUK AMPLIFIKASI GEN katG MULTIDRUG RESISTANCE TUBERCULOSIS (MDR-TB) DENGAN METODE POLYMERASE CHAIN REACTION (PCR) Putu Tedi Suryadi; Ketut Ratnayani; Sagung Chandra Yowani
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Tuberculosis, the world’s major diseases, is one of the emerging infectious disease. The tuberculosis problem has become complicated and burdensome due to the emergence of drug resistant such as isoniazid (INH) resistant strains of Mycobacterium tuberculosis. Mutation in katG gene is the main mechanism of INH-resistance in most strains. Amplification of M. tuberculosis katG gene was performance by using PCR for detect the mutation. A pair of specific PCR primers (forward and reverse) was the most important factor to limit the target region of amplification. Primer designing is preceedly carried out for producing the specific primer desired. The aim of this study was to design the specific primers for a fragment of katG gene. In silico primer design was carried out by using Clone Manager Suite 6. DNA sequence template used in this primer design was downloaded from www.ncbi.nml.nih.gov. M. tuberculosis H37Rv katG gene with genbank code X68081.1 was choosen. This study was successfully designed the forward primer (5' GAAGTACGGCAAGAAGCTCTC 3') and reverse one (5' CGTGATCCGCTCATAGATCG 3') which was length of 21 and 20 nucleotide, respectively. These pair of primers were meet the requirement of a good primer include primer length, Tm value, percentage of GC content, no hairpins, limited dimers and runs. In conclusion, the result of this research showed that the primer designed were acceptable to amplify the fragment of 724 bp of katG gene in silico.

KAJIAN PENGARUH VARIASI KONSENTRASI ASAM SITRAT TERHADAP KEKUATAN GEL PRODUK GELATIN KULIT AYAM BROILER DIKAITKAN DENGAN POLA PROTEINNYA Tutut Hardikawati; Ni Made Puspawati; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Gelatin is a biopolymer that can be generated from partially hydrolysis of collagen tissue. Extraction of gelatin consists of pretreatment and thermal extraction steps. Pretreatment process used sodium hydroxide to remove non collagen protein in matrix sample, sulfuric acid to demineralize, and citric acid to hydrolyse. The aim of this research was to study the effect of variation in concentrations of citric acid used in hydrolysis process on the gel strength and protein profile of gelatin products extracted from broiler chicken skin. The variation of the concentration citric acid used was 0.7 % (GA); 1.5% (GB); and 3.0% b/v (GC) respectively. The gel strength was measured using CT3 Texture Analyzer and protein profile of gelatin product was analyzed by SDS-PAGE method. The result showed that variation in concentration of citric acid used in the pretreatment process affected the gel strength and protein profile of gelatin product. Increasing the concentration of citric acid used in pretreatment process decreased the gel strength and molecular weight of gelatin product. Gel strength of each gelatin product was 265.81 g bloom for GA ; 196.05 g bloom for GB (1.5%), and 35.32 g bloom for GC (3.0 %) respectively. The electropherogram of both GA (0.7%) and GB (1.5%) revealed similar pattern of protein bands but the thickness of each bands was different. On the other hands, GC (3.0%) did not show any protein bands on the eletropherogram. The best gelatin product obtained in this experiment was found by using 0.7 % b/v citric acid (GA) in the pretreatment process. The gelatin product (GA) had characteristics as follows: yield 15.73%; moisture 7.30%; ash 0.51%; protein content 97.95%; fat content 0.62%; gel strength 265. 81 g bloom and thicker protein bands than others.

ISOLASI DAN IDENTIFIKASI SENYAWA GOLONGAN FLAVONOID DARI MADU K ELENGKENG (Nephelium longata L.) Ida Ayu Raka Astiti Asih; Ketut Ratnayani; Ida Bagus Swardana
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 1 Januari 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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The determination of anti free radical activity on longan honey (Nephelium longata L.) by DPPH method using UV-Vis sphectrophotometry and identification of chemical compound in non polar and semi polar fraction have been done. Longan honey was diluted with methanol and then partied by n-hexane and ethyl acetate. The absorbance was measured at 497 nm, 517 nm, and 537 nm for the DPPH concentration of : 0,001%, 0,002%, 0,003%, and 0,004% and the chemical compound was identified by phytochemical method.The result showed that part of n-hexane and ethyl acetate probably consist of chemical compound of isoflavone and value of anti free radical activity on longan honey in semi polar fraction was higher than in non polar fraction which were 91,71% and 77,68% at DPPH concentration of 0,001% (b/v).

KAPASITAS ADSORPSI BEBERAPA JENIS KULIT PISANG TERAKTIVASI NaOH SEBAGAI ADSORBEN LOGAM TIMBAL (Pb) Putu Eka Purnama; I Gusti Ayu Kunti Sri Panca Dewi; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Adsorption capacity of various types of banana skin (green, kepok and susu) activated with NaOH as adsorbents for lead (Pb) has been studied. This research consisted of several steps included determination of the surface area of activated and unactivated adsorbents, equilibrium time, adsorption isotherms and adsorption capacity of adsorbents from green, kepok and susu banana skins activated with NaOH . The results showed that adsorbents from green, kepok and susu banana skin activated with NaOH had surface were of 36.2181 m2/g, 35.5531 m2/g and 35.8378 m2/g respectively. On the other hand, the surface area of unactivated adsorbents of green, kepok and susu banana skin were 35.3105 m2/g, 35.3199 m2/g, and 35.7928 m2/g respectively. Equilibrium time for green, kepok and susu banana skin adsorbents activated with NaOH were 30; 30 and 90 minutes. Adsorption isotherms of adsorbents from green, kepok and susu banana skin activated with NaOH were at concentration 100 ppm. Adsorption capacity of activated adsorbents from green banana, kepok banana and susu banana skin were 7.022 mg/g, 5.3078 mg/g and 6.6850 mg/g respectively.

PENGARUH PENAMBAHAN SUSU SKIM TERHADAP HASIL DNA METAGENOMIK DIISOLASI DARI TANAH HUTAN MANGROVE Ni Putu Frida Oktaningtias Widiarthi; Ketut Ratnayani; I Nengah Wirajana
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Cell lysis is the most important step for quality and quantity of metagenomic DNA isolated from an environmental samples. The aim of the research was to compare the quality (integrity and purity) of the metagenomic DNA isolated using the direct cell lysis method from mangrove forest soil with and without skim milk. The total of metagenomic DNA isolated from mangrove forest soil result was analyzed by the spectrophotometric UV-Vis method at ? 230, 260, and 280 nm; and also by using the agarose gel electrophoresis. The results showed that metagenomic DNA can be isolated from mangrove forest soil. The agarose gel electrophoresis results showed that the total DNA quality obtained by the direct cell lysis using buffer lysis with skim milk was relatively less fragmented and the band intensity of DNA was higher compared with direct cell lysis using buffer lysis without skim milk. The results of spectrophotometry indicated that the purity of DNA isolated with and without skim milk was not significantly different against the humic acid (ratio on A260/230). As shown by the A260/280 ratio, the total DNA isolated without skim milk had higher purity level than with skim milk.

Co-Authors A. A. Ayu Septri Juwarni A. W. Dwiputri Acintya, N. M. T. C. Amanda Awalia Ramadhani Ana Listya Anak Agung Istri Agung Mayun Laksmiwati Ariati, N. K. Darma Asih Yuliana Deniariasih, N.W. Dewi, I G. A. K. S. P. Dita Rizkiyanti Helen Helda Prastika I G. A. M. A. S. Gitadewi I Gusti Ayu Kunti Sri Panca Dewi I Ketut Suter I Made Oka Adi Parwata I Nengah Kencana Putra I Nengah Wirajana I Wayan Sudiarta I Wayan Suirta I. A. Preharsini Kusuma I. A. R. Astiti Asih Ida Bagus Swardana Indriani Wisnu Susanto Panjaitan James Sibarani Lia Kusumaningrum Liangky Syane S Listiyanti, N. K. L. M. A. Pratiwi M. Nazib Made Arsa Maman Sudiarto Ni G. A. M. Dwi Adhi Suastuti Ni Komang Ariati Ni Luh Putu Mega Wahyuni Ni Made Puspawati Ni Made Suaniti Ni P. Indah Septian P. Ni Putu Frida Oktaningtias Widiarthi Ni Putu Wiwik Oktayuni Ni Wayan Agustini Ni Wayan Wisaniyasa NYOMAN SEMADI ANTARA Putra, A. A. B. Putu Eka Purnama Putu Lita Astriani Putu Setya Sri Antary Ro’yal Aini Sagun Chandra Yowani Suarsa, I W. Syamsul Arifin Tutut Hardikawati

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