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All Journal Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Indonesia Medicus Veterinus Jurnal Ilmu dan Industri Peternakan Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) As-syifaa Jurnal Farmasi ARSHI Veterinary Letters
Fedri Rell
Hasanuddin University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Ilmu dan Kesehatan Hewan

 1 
Virulensi Virus Newcastle Disease Isolat Lapang Berdasarkan Analisis Bioinformatika Gen Protein Hemaglutinin - Neuraminidase Fedry Rell; Anak Agung Mirah Adi; I Gusti Ngurah Kade Mahardika
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

Newcastle disease virus (NDV) is very contagious disease agent, and causes many outbreaks trough out thecountry of Indonesia. This study was conducted to determine the virulence of Bali field isolate of NDVbased on haemaglutinin-neuraminidase (HN) protein genes. Four field isolates from different locationswere propagated at fertilized chicken eggs of 9 days old. Allantoic fluid was harvested and NDV wasconfirmed using standard haemaglutination (HA) and haemaglutination inhibition test (HI) methods. Thefragment of HN protein gene was amplified using RT-PCR. The product was sequenced using Big-Dyetermination method. All four isolates grew well with the titer of 2[5]-2[9] HA unit and could be confirmedusing HI test. The HN genes, however, exhibited variations at its 3-D structure and hydrophobicity betweenthe virus that previously circulated in Indonesia and vaccine virus. It is concluded that all four Bali’sisolates under this study are virulent VND of the genotype VII. Further testing is needed to justify the bestformula of NDV vaccine to be used trough out Indonesia.
Polimorfisme Lokus Mikrosatelit D10S1432 pada Populasi Monyet Ekor Panjang Di Sangeh F. Rell; S.K. Widyastuti; I N. Wandia
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Abstract

Genetic polymorphisms are variations in the genetic structure of populations that represents the base level of biodiversities.  This research aimed to assess the polymorphism of microsatellite locus of D10S1432 in the population of long-tailed macaques in Sangeh, Bali.  A total of 18 blood samples collected as a source of DNA. DNA was extracted using QIAmp® DNA Blood Mini Kit. Microsatellite locus was amplified by polymerase chain reaction technique (PCR) with a total of 30 cycles, and a 57oC of annealing temperature. Furthermore, the alleles were separated by 7% polyacrylamide gel electrophoresis, and emerged with silver staining. The results showed that there were three types of alleles found at D10S1432 locus in the long-tailed macaques population in Sangeh. The alleles size varied from 170 bp to 186 bp. The allele frequencies were  0.28, 0.67, and 0.05 for allele 170, allele 174, and allele 186 consecutively. The heterozygosity of the locus was 0.48. It can be concluded that microsatellites locus D10S1432 is polymorphic in the long-tailed macaques population in Sangeh.
Co-Authors A. Rianti Rhasinta Alifha Abdul Wahid Jamaluddin Adryani Ris Amriani, Rini Anak Agung Ayu Mirah Adi Anak Agung Putu Joni Wahyuda Andi Magfira Satya Apada Andi Magfira Satya Apada Andi Magfira Satya Apada Andi Muhammad Yahya Andi Nurul Atiqa Faizah Makkarumpa Anugrah, Dian Apada, Andi Magfira Satya Aprilia, Eka Astri Caturutami Sjahid Baso Yusuf Ciputra, Lyvia Angeline Danawir Alwi Dariming, Nur Azizah Dwi Kesuma Sari DWI SURYANTO Dwijaya, Melkiedek Jeffry Frinth Azarya Kuriakos Ngopo Husada, Anggini Putri I Gusti Ngurah Kade Mahardika i Nengah Wandia Ikliptikawati, Dini Kurnia Ishika Maulyda Jalil, Muhammad Fikri Raditya Jamilah, Ainun Kadek Evi D.P. Dewi Mapparenta, Bulan Allu Masyita Muhammad Alamsyah Muhammad Fadhlullah Mursalim Muhammad Fadhlullah Mursalim Muhammad Fath Maulana Binar Uskar Muhammad Fathiyakan Thamrin Muhammad Muflih Nur Mursalim, Muhammad Fadhlullah Muslimin, Ihram Kautsar Nandar Hidayat Nugroho, Genna Prama Nurfadhillah, Besse Nurisma Devi Wahyuningsih Purba, Fika Yuliza Putri Dyah Zahrani Rahman, Andi Simpurusiang Rasdiyanah Rian Hari Suharto RR. Ella Evrita Hestiandari Ruhil Aziz Sarah Saputri, Aditya Dwi Sarah, Ruhil Aziz Satya Apada, Andi Magfirah Sri Kayati Widyastuti Suharto, Rian Hari Syahbar, Lutfiah Kurnia Toliu, Wudhia Windy Trini Purnamasari Syahid Vinka Melani Putri Zainal Abidin Kholilullah Zainal Abidin Kholilullah Zulfikri Mustakdir