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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Indonesian Journal of Electrical Engineering and Computer Science Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
M. Zairin Junior
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Social Sciences Veterinary Other

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
Optimasi transplantasi menggunakan sel donor dari ikan gurame muda dan ikan nila triploid sebagai resipien Alimuddin .; M. Zairin Junior; Harton Arfah
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 3 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Testicular cell transplantation technology can be used in fish seed production engineering. In this study, optimization of transplantation using donor cells from young gouramy and triploid tilapia (3N) as recipient. Triploid tilapia is produced using heat shock method. The testes of male gouramy (body weight of 400- 850 g) was dissociated using 0.5% trypsin. Dissociated testicular cells was injected into the peritoneal cavity of tilapia larvae. Analysis of donor cell colonization was carried out using PCR method with DNA template that had been extracted from the gonad of 2-month-old tilapia. PCR was performed using specific primers for the growth hormone gene and (3-actin as an internal control of DNA loading. The results of nucleoli preparation showed that the success of triploidyzation was 88.5%. The gonad size of diploid (2N) and 3N recipient were relatively similar, while in not transplanted 3N tilapia was rudimentary. PCR results showed that the transplanted 3N tilapia has a DNA band of the same size with gouramy, while in control was not. This indicated that donor cells have been colonized in the gonads of recipient. The donor cell colonization in recipient 3N (78%) was higher than that of 2N (50%). Further research is required to determine the ability of donor cells differentiate into sperm and eggs in recipient gonad.
Co-Authors , Alimuddin . Hermawan . Sukenda . Suriansyah . Syafiuddin . Tarsim ., Mulyasari A M Tahya A S Mubarak A. Oman Sudrajat A. Shofy Mubarak A. Yunianti Aarab, Zineb Ade Sunarma Ade Sunarma Ade Sunarma, Ade Adharto Utiah Agus O . Sudradjat Agus Oman Sudrajat Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin . Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin alimuddin alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anouar Darif Aprelia Martina Tomasoa, Aprelia Martina Arroub, Omar Asep Sopian Avarre, Jean-Christophe Azis Azis Bambang Purwantara Benny Heltonika Bethsy J. Pattiasina Cahyono, Tatak Dwi D. Nurlestiyoningrum D.S. Sjafei Damiana Rita Ekastuti DEDI JUSADI Dian Hardiantho Dian Hardianto Didik Ariyanto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dita Puji Laksana Dwi Hany Yanti E. Riani Eddy Supriyono Enang Harris Surawidjaja Etty Riani Fajar Basuki Fajar Maulana . Farizah, N Fauzan, Agung Luthfi Flandrianto S. Palimirmo GORO YOSHIZAKI H. Arfah Hamsah Hamsah Harton Arfah I Khasani I MADE ARTIKA I Wayan Nurjaya I. Herviani I. Mokoginta I. Supriatna Iis Diatin Ikhsan Khasani Imron Imron, Imron Irma Andriani IRMA ANDRIYANI Irvan Faizal Irzal Effendi Iskandar, Andri Ita Djuwita Jamaluddin Jompa Jasmanindar, Yudiana Jean-Christophe Avarre Julie Ekasari K Nisaa K. Sumantadinata Kadarusman . Khairun Nissa Khasani, I Komar Sumantadinata Kukuh Adiyana Kukuh Nirmala Kusman Sumawidjaja Laurent Pouyaud Livana Dethris Rawung Lolita Thesiana M Yamin M Yamin M. Bintang M. M. Raswin M. Raswin M. Sakdiah M. Syukur M. Toelihere M. Tri Djoko Sunarno M.M. Raswin Meilisza, Nina Mia Setiawati Mozes R . Toelihere MOZES R. TOELIHERE Mubarak, A S MUHAMMAD AGUS SUPRAYUDI Muhammad Muhammad Muhammad Safir Mulyasari . Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim N Farizah N. Potalangi N.B.P Utomo Nababan, Yanti Inneke Nasrullah, Hasan Naufal, Muhammad Restya Neviaty P. Zamani Nisaa, K Nugraha, Media Fitri Isma Nunak Nafiqoh Nur Bambang Priyo Utomo Nurbariah Nurbariah O. Carman Odang Carman Odilia Rovara Poppy Dea Bertha, Poppy Dea Prassetyo Dwi Dhany Wijaya Purnamawati Purnamawati Purnamawati Purnamawati Putri Zulfania, Putri R. Affandi R.G. Pahlawan R.K. Sari R.R Sri Pudji R.R.S.P.S. Dewi Rahmani, My Driss Rahminiwati, Min Rakhmawati, Rakhmawati, RIDWAN AFFANDI Robin . Robin . Roza Elvyra S. Darwisito S. Dewi S. Handayani S. Mulyati S. Purwati Safira Qisthina Ayuningtyas, Safira Qisthina Siti Subaidah Sri Nuryati Sri Nuryati Srihadi Agungpriyono Suci Antoro Sugeng Budiharsono Sujaka Nugraha Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Syafyudin Yusuf T.L. Yusuf Tahya, A M Tarsim Tarsim TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Triayu Rahmadiah Tuty L . Yusuf W. Manalu Wahyu Pamungkas Wasmen Manalu WIDANARNI WIDANARNI Wisriati Lasima Wiyoto Wiyoto Wiyoto, W. Y. Hadiroseyani Y. Yustikasari Yogi Himawan Yudha Trinoegraha Adiputra Yusnarti Yus Zahri, Abdul